Human or rat microsomal 5 alpha-reductase activity, as measured by enzymic conversion of testosterone into alpha-dihydrotestosterone binding a competitive inhibitor, [3H]17 beta-NN-diethulcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA) to the reductase, is inhibited low concentrations (less than 10 microM) certain polyunsaturated fatty acids. The relative inhibitory potencies unsaturated acids are, in decreasing order: gamma-linolenic acid greater...

Rat brain cortex membranes bind to a conjugate of substance P and 125I-labeled Bolton-Hunter reagent, this binding can be inhibited by low concentration (Kd = 1.2 +/- 0.4 X 10(-8) M). This is reversible saturable (0.5 0.1 pmol sites/mg protein). Fragments as small the carboxyl-terminal hexapeptide inhibit although their potency decreases with decrease in length peptides. The affinities smaller peptides or which amide amino acids are removed drastically reduced. Biologically active analogs P,...

Materials-[4-14C]Testosterone (51.9 mCi/mmol), [1,2,6,7,16,17-3H]testosterone (132 Ci/mmol), [1,2,4,5,6,7,16,17-3H]5a-dihydrotestosterone (200 and Aquasol-2 were purchased from New England Nuclear.Ethyl acetate, HPLC grade, was J. T. Baker Chemical Co., cyclohexane MCB Reagents (Cincinnati, OH).Plates for thin layer chromatography LK5DF plates Whatman.Cell culture medium 199 Gibco (Grand Island, NY) Triton X-100 Sigma.The Coomassie brilliant blue dye reagent protein determination...

The abbreviations and trivial names used are: testosterone (17phydroxy-4-androsten-3-one); 5a-dihydrotestosterone (5a-androstan-17P-ol-3-one); DMAA, I7~-N,N-diethylcarbaoyl-4-methyl-4-aza-5a-androstan-3-one; cortisol (4-pregnen-ll~,l7a,21-triol-3,20-dione); estradiol (1,3,5( lO)-estratriene-3,17fl-diol); progesterone (4-pregnen-3,20-dione); cimetidine (N"-cyano-N-methyl-N"[2-(5-methyl-imidazol-4-yl)methylthioethyl]guanidine; spironolactone...

Eledoisin and substance P are members of a class peptides termed tachykinins. They share similar spectrum biological activities but their relative potencies in various pharmacological assays differ. We have investigated whether there is more than one receptor for these tachykinins rat brain cortex membranes. 125I-Bolton Hunter-conjugated eledoisin specifically binds to membranes with high affinity. The binding inhibited over 95% by unlabeled (6.6 microM). Scatchard analysis the this ligand...

A conjugate (125I-BH-SP) between substance P and 125I-labeled Bolton-Hunter reagent binds reversibly to a single class of high affinity (Kd = 4 nM) binding sites on dispersed rat parotid cells. The total number is 49 +/- 17 fmol/mg protein. 13 fragments analogs correlates with their relative potency in stimulating salivation. smallest fragment which exhibits significant saliva-stimulating activity the COOH-terminal hexa(6-11)peptide. Structurally unrelated neurotransmitters hormones do not...

The initiation of protein synthesis by ribosomal particles rat ventral prostate was studied measuring binding an initiator (35-S)methionyl-tRNAf. activity is dependent on ribosomes, GTP, and a cytosol fraction. 40S but not the 60S subunit are active. decreases rapidly within one hour after castrated. This loss prevented intraperitoneal injection 17beta-hydroxy-5-alpha-androstan-3-one (5-alpha-dihydrotestosterone). can be stimulated almost immediately (within 10 min) intravenous low dose (15...

ABSTRACT Sexual behaviour was induced in castrated male rats with oestradiol-17β- or testosterone-filled constant-release implants. Testosterone-induced sexual unaffected by treatment the 5α-reductase inhibitor 17β-N,N-diethylcarbamoyl-4-aza-5α-androstan-3-one (4-MA; 16·7 mg/day) but aromatization 1,4,6-androstatriene-3,17-dione (ATD; 10 prevented testosterone from inducing behaviour. could be activated treated plus ATD 4-MA implants filled a low dose of oestradiol. Lordosis ovariectomized...

Despite intensive research on Parkinson disease (PD) for decades, this common neurodegenerative remains incurable. We hypothesize that abnormal iron accumulation is a thread underlying the emergence of hallmarks PD, namely mitochondrial dysfunction and α-synuclein accumulation. investigated powerful action main regulator hepcidin in brain. In both rotenone 6-hydroxydopamine models overexpression by means virus-based strategy prevented dopamine neuronal loss suppressed major pathologies...

The studies of number sense in different species are severely hampered by the inevitable entanglement non-numerical attributes inherent nonsymbolic stimuli representing numerosity, resulting contrasting theories numerosity processing. Here, we developed an algorithm and associated analytical methods to generate that not only minimized impact magnitudes perception but also allowed their quantification. We trained number-naïve rats with these as sound pulses two or three numbers demonstrated...

Abstract In order to improve the bioavailability of baicalin, this article prepared for α- zein loaded with baicalin composites ( zein@BA) by pH driven method and they were characterized using scanning electron microscopy, infrared spectroscopy, measurement particle size distribution in water solution phase techniques. The digestive behavior antioxidant activity before after simulating gastrointestinal fluid vitro studied as well. At same time, molecular dynamics simulation techniques used...

Background/Aims: Ketamine is a widely used anesthetic in obstetric and pediatric anesthesia. In the developing brain, widespread neuron apoptosis triggered by ketamine has been demonstrated. However, little known about its effect on neural stem cells (NSCs) function. This study aimed to investigate of proliferation NSCs from neonatal rat hippocampus. Methods: Neural were isolated hippocampus Sprague-Dawley rats postnatal day 3. dose-response experiments, cultured exposed different...

Music exposure is known to play a positive role in learning and memory can be complementary treatment for anxiety fear. However, whether juvenile music affects adult behavior not known. Two-week-old Sprague-Dawley rats were exposed 2 hours daily or background noise (controls) period of 3 weeks. At 60 days age, subjected auditory fear conditioning, extinction training, anxiety-like assessments anterior cingulate cortex (ACC) brain-derived neurotrophic factor (BDNF) assays. We found that the...

Renal denervation (RDN) has been shown to be safe and efficacious in reducing blood pressure patients with resistant hypertension. In the present study, we describe a first-in-man report of Iberis renal device patient who underwent two RDN procedures: one via femoral artery transulnar approach. was performed patient's right using an catheter approach, as transfemoral or transradial arteries could not approached due their anatomic constraints. summary, approach is feasible represents...

21-Diazo-4-methyl-4-aza-5 alpha-pregnane-3,20-dione (Diazo-MAPD) inhibits steroid 5 alpha-reductase in liver microsomes of female rats with a Ki value 8.7 +/- 1.7 nM, and the inhibition is competitive testosterone. It also binding inhibitor, [3H] 17 beta-N,N-diethylcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA), to enzyme microsomes. The activity inhibitor by diazo-MAPD becomes irreversible upon UV irradiation. [1,2-3H]Diazo-MAPD binds single high affinity site (Kd 8 125 pmol...

Clinical observations reveal that rapid eye movement (REM) sleep behavior disorder (RBD) often develops prior to alpha-synucleinopathies including Parkinson's disease (PD). However, a causal relationship between alpha-synucleinopathy and Parkinsonian neurodegeneration has not been delineated.Rats were chronically treated with rotenone EEG EMG signals recorded for analysis of behavior, assisted by video recording body movements. C-fos expression TUNEL staining used assess neuronal activation...

Cushing's syndrome (CS) during pregnancy is rare. It causes the clinical disorder by overproduction of cortisol. Hypercortisolemia in can lead to severe complications, both for mother and fetus, including spontaneous abortion, perinatal death, prematurity, maternal hypertension, heart failure, diabetes opportunistic infections. The most common cause hypercortisolemia a cortisol-secreting adrenal tumor. Herein we present 31 year-old female patient, at 20 weeks' gestation, with CS secondary...