A5016275619- Race, History, and American Society
- Adipose Tissue and Metabolism
- Algal biology and biofuel production
- Molecular Biology Techniques and Applications
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Latin American and Latino Studies
- American Environmental and Regional History
- Peroxisome Proliferator-Activated Receptors
- Genetics, Bioinformatics, and Biomedical Research
- Lipid metabolism and biosynthesis
- Cuban History and Society
- Advanced Fluorescence Microscopy Techniques
- Caribbean history, culture, and politics
- Diverse Education Studies and Reforms
- American and British Literature Analysis
- Adipokines, Inflammation, and Metabolic Diseases
- Nuclear Structure and Function
- American History and Culture
- Micro and Nano Robotics
- Neural dynamics and brain function
- Critical Race Theory in Education
- Neuroscience and Neural Engineering
- Machine Learning in Materials Science
University of Minnesota
2013-2025
Imaging Center
2012-2025
University of Geneva
2023-2024
Institute of Biomedical Sciences, Academia Sinica
2024
Kaiser Permanente Fontana Medical Center
2024
Kaiser Permanente
2023
University of Minnesota System
2019
Twin Cities Orthopedics
2009-2019
Regis University
2014
May Institute
2011
Cytoplasmic dynein is a multisubunit minus-end–directed microtubule motor that serves multiple cellular functions. Genetic studies in Drosophila and mouse have demonstrated function essential metazoan organisms. However, whether the of reflects mitotic requirement, what specific tasks require remains controversial. an excellent genetic system which to analyze mitosis, providing cytology embryonic somatic cells. We used previously characterized recessive lethal mutations heavy chain gene,...
Chlamydomonas cells excise their flagella in response to a variety of experimental conditions (e.g., extremes temperature or pH, alcohol detergent treatment, and mechanical shear). Here, we show that flagellar excision is an active process whereby microtubules are severed at select sites within the transition zone. The zone located between axoneme basal body; it characterized by pair central cylinders have H shape when viewed longitudinal section. Both connected A tubule each microtubule...
ABSTRACT A human cDNA expression library was screened using anti-centrin antibodies to obtain a clone encoding centrin. The contains an open reading frame of 516 base pairs and predicts product 172 amino acids with calculated molecular mass 19,528 pI 4.61. Sequence analysis demonstrates that centrin centrins from higher plants, protozoa, algae, Xenopus the yeast CDC31 gene are closely related members subfamily EF-hand superfamily calcium-binding proteins. sequence has four putative domains...
Compartmentalized co-localization of enzymes and their substrates represents an attractive approach for multi-enzymatic synthesis in engineered cells biocatalysis. Sequestration would greatly increase reaction efficiency while also protecting host from potentially toxic intermediates. Several bacteria form protein-based polyhedral microcompartments which sequester functionally related regulate access to other small metabolites. Such bacterial may be into nano-bioreactors, provided that they...
The GPCRDB is a Molecular Class-Specific Information System (MCSIS) that collects, combines, validates and disseminates large amounts of heterogeneous data on G protein-coupled receptors (GPCRs). contains experimental sequences, ligand-binding constants, mutations oligomers, as well many different types computationally derived such multiple sequence alignments homology models. provides access to the via number methods. It offers visualization analysis tools, query systems. updated...
When Chlamydomonas cells are deflagellated by pH shock or mechanical shear the nucleus rapidly moves toward flagellar basal apparatus at anterior end of cell. During regeneration returns to a more central position within The is connected system fibers, roots (rhizoplasts), which undergo dramatic contraction that coincides with nuclear movement. A corresponding extension root system, back its preshock configuration observed as retracts position. Anterior displacement and require free calcium...
Abstract Affinity‐purified antibodies that recognize the 20,000‐dalton molecular weight (20 kd) striated flagellar root protein of Tetraselmis striata have been used to identify antigenic homologs in other eucaryotic organisms diverse evolutionary origins. Among green algae, and Chlamydomonas , their colorless relative, Polytomella 20‐kd appear associated with basal bodies. This occurs most prominnently form roots both microtubule subtended types. cultured mammalian cells (PtK2 primary mouse...
A variety of microscopy techniques are used by researchers in the life and biomedical sciences. As these become more powerful complex, it is vital that scientific articles containing images obtained with advanced microscopes include full details about how each image was obtained. To explore reporting such we examined 240 original research published eight journals. We found quality poor, some no information were obtained, many lacking important basic details. Efforts researchers, funding...
In the unicellular alga Chlamydomonas, two anterior flagella are positioned with 180° rotational symmetry, such that beat effective strokes in opposite directions (Hoops, H.J., and G.B. Witman. 1983. J. Cell Biol. 97:902–908). The vfl1 mutation results variable numbers positioning of basal bodies (Adams, G.M.W., R.L. Wright, J.W. Jarvik. 1985. 100:955–964). Using a tagged allele, we cloned VFL1 gene encodes protein 128 kD five leucine-rich repeat sequences near NH2 terminus large...
Adipocyte fatty acid-binding protein (AFABP/aP2) forms a physical complex with the hormone-sensitive lipase (HSL) and AFABP/aP2-null mice exhibit reduced basal hormone-stimulated lipolysis. To identify determinants affecting interaction fluorescence resonance energy transfer (FRET) imaging was used in conjunction mutagenesis strategy to evaluate roles AFABP/aP2 acid binding HSL phosphorylation have formation as well determine site on AFABP/aP2. The nonfatty mutant of (R126Q) failed form...
Molecular disruption of the lipid carrier AFABP/aP2 in mice results improved insulin sensitivity and protection from atherosclerosis. Because small molecule inhibitors may be efficacious defining mechanism(s) action, a chemical library was screened identified 1 (HTS01037) as pharmacologic ligand capable displacing fluorophore 1-anilinonaphthalene 8-sulfonic acid binding cavity. The X-ray crystal structure bound to revealed that binds at structurally similar position long-chain fatty acid....
Agrochemical delivery is highly inefficient, and novel application methods are necessary to promote crop health yields while reducing environmental impact. In this work, a vacuum infiltration seed priming strategy was developed incorporate silica nanoparticles into soybeans. Although successful in initial greenhouse field studies, little known about the amount of nutrient being delivered conditions for optimum accumulation. Herein, various were evaluated using fluorescent confocal...
Abstract Pathogenic mycobacterial organisms have the capacity to inhibit macrophage activation and phagosome maturation. Although mechanism is complex, several studies incriminated signaling through TLR2 receptors with subsequent of MAPK pathway p38 (MAPKp38) overproduction IL-10 in survival pathogenic organisms. In present study, we compared response bovine monocytes infection by Mycobacterium avium subspecies paratuberculosis (MAP), cause ruminants, closely related organism M. (Maa), which...
Besides the secretion of fatty acids, lipolytic stimulation adipocytes results in triglyceride-rich extracellular vesicles and some free proteins (e.g., acid binding protein 4) that, sum, affect adipose homeostasis as well development metabolic disease. At mechanistic level, signals activate p53 an triglyceride lipase–dependent manner, pharmacologic inhibition attenuates adipocyte-derived vesicle (AdEV) FABP4 secretion. Mass spectrometry analyses secretome identified involved glucose...
Previous in vitro studies have established that hormone sensitive lipase (HSL) and adipocyte fatty acid-binding protein (AFABP) form a physical complex presumably positions the FABP to accept product acid generated during catalysis. To assess AFABP-HSL interaction within cellular context, we used lipocytes derived from 293 cells (C8PA cells) examined association using fluorescence resonance energy transfer. Transfection of C8PA with cyan fluorescent (CFP)-HSL, yellow (YFP)-adipocyte FABP, or...
Abstract We have used two monoclonal antibodies to demonstrate the presence and localization of actin in interphase mitotic vegetative cells green alga Chlamydomonas reinhardtii. Commercially available raised against smooth muscle (Lessard: Cell Motil. Cytoskeleton 10:349–362, 1988; Lin: Proc. Natl. Acad. Sci. USA 78:2335–2339, 1981) identify as a ∼43,000‐M r protein by Western immunoblot procedures. In an earlier study, Detmers coworkers ( Motil . 5:415–430, 1985) first identified using...
Background. Pancreatic acinar cells are commonly cotransplanted along with islets during auto- and allotransplantations. The aims of this study were to identify how cell proteases cause human islet loss before after transplantation impure preparations prevent improve function supplementation α-1 antitrypsin (A1AT). Methods. Acinar protease activity, insulin levels, percent measured culture pure clinical preparations. effect on ultrastructure β-cell granules examined by transmission electron...
The hormone-sensitive lipase (HSL) and adipocyte fatty acid-binding protein (AFABP/aP2) form a physical complex that affects basal hormone-stimulated acid efflux. Previous work has established AFABP/aP2-HSL formation requires HSL be in its activated, phosphorylated AFABP/aP2 have bound acid. To identify the binding site of combination alanine-scanning mutagenesis fluorescence resonance energy transfer was used. Mutation Asp17, Asp18, Lys21, or Arg30 (but not other amino acids...
Abstract Microalgae have been shown as a potential bioresource for food, biofuel, and pharmaceutical products. During the growth phases with corresponding environmental conditions, microalgae accumulate different amounts of various metabolites. We quantified neutral lipids accumulation analyzed swimming signatures (speed trajectories) motile green alga, Dunaliella primolecta , during lag–exponential–stationary cycle at nutrient concentrations. discovered significant changes in lipid content...
Anti-retroviral therapy (ART) generally suppresses HIV replication to undetectable levels in peripheral blood, but immune activation associated with increased morbidity and mortality is sustained during ART, infection rebounds when treatment interrupted. To identify drivers of potential sources viral rebound, we modified RNAscope situ hybridization visualize HIV-producing cells as a standard against which compare the following assays virus rebound interruption: (i) envelope detection by...