A5082247691- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Image Processing Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Optical Imaging and Spectroscopy Techniques
- Immune Cell Function and Interaction
- Colorectal Cancer Treatments and Studies
- Immunotherapy and Immune Responses
- Genetics, Bioinformatics, and Biomedical Research
- Near-Field Optical Microscopy
- Molecular Biology Techniques and Applications
- Medical Imaging Techniques and Applications
- RNA Interference and Gene Delivery
- Digital Holography and Microscopy
- Estrogen and related hormone effects
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- Functional Brain Connectivity Studies
- Cellular transport and secretion
- Advanced Neuroimaging Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
University of Minnesota
2016-2025
Minnesota Supercomputing Institute
2024
University of Minnesota System
2017-2023
University of Minnesota Medical Center
2018-2022
Imaging Center
2016-2022
Centre for Genomic Regulation
2013-2017
Twin Cities Orthopedics
2016
Universidad de Navarra
2007-2014
École Polytechnique Fédérale de Lausanne
2012-2014
Abstract Magnetic resonance imaging (MRI) has transformed our understanding of the human brain through well-replicated mapping abilities to specific structures (for example, lesion studies) and functions 1–3 task functional MRI (fMRI)). Mental health research care have yet realize similar advances from MRI. A primary challenge been replicating associations between inter-individual differences in structure or function complex cognitive mental phenotypes (brain-wide association studies...
Significance The bacterial cytoskeletal protein FtsZ, which forms a constricting “Z-ring” during cell division, is the major involved in division almost all prokaryotes, and key next-generation antibiotic target. However, small size of Z-ring, approximately 500 nm diameter, makes it difficult to observe vivo. We provide quantitative nanoscale picture Z-ring organization live cells; these results improve our understanding structural force-generating roles FtsZ division. To achieve this, we...
Abstract DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes such adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine structure Mycoplasma pneumoniae at a 10 kb resolution. We find structure, with global symmetry between two arms that connect opposite poles, one bearing chromosomal Ori other...
Cytoplasmic dynein is essential in motoneurons for retrograde cargo transport that sustains neuronal connectivity. Little, however, known about dynein's function on the postsynaptic side of circuit. Here we report distinct roles at neuromuscular junctions (NMJs). Intriguingly, show punctae accumulate postsynaptically glutamatergic synaptic terminals. Moreover, Skittles, a phosphatidylinositol 4-phosphate 5-kinase produces PI(4,5)P2 to organize spectrin cytoskeleton, also localizes...
The extracellular matrix (ECM) in idiopathic pulmonary fibrosis (IPF) drives progression; however, the ECM composition of fibroblastic focus (the hallmark lesion IPF) and adjacent regions remains incompletely defined. Herein, we serially sectioned IPF lung specimens constructed into tissue microarrays immunostained for components reported to be deregulated IPF. Immunostained sections were imaged, anatomically aligned, 3D reconstructed. myofibroblast core (defined by collagen I, α-smooth...
Gene editing with CRISPR/Cas9 is revolutionizing biotechnology and medical research, yet affordable, efficient, tailorable delivery systems are urgently needed to advance translation. Herein, a series of monodisperse amphiphilic block polymers poly[ethylene oxide-b-2-(dimethylamino) ethyl methacrylate-b-n-butyl methacrylate] (PEO-b-PDMAEMA-b-PnBMA) that housed three PEO lengths (2, 5, 10 kDa) variant lacking (PDMAEMA-b-PnBMA) were synthesized via controlled radical polymerization assembled...
A variety of microscopy techniques are used by researchers in the life and biomedical sciences. As these become more powerful complex, it is vital that scientific articles containing images obtained with advanced microscopes include full details about how each image was obtained. To explore reporting such we examined 240 original research published eight journals. We found quality poor, some no information were obtained, many lacking important basic details. Efforts researchers, funding...
Naive CD4+ T lymphocytes differentiate into various Th cell subsets following TCR binding to microbial peptide:MHC class II (p:MHCII) complexes on dendritic cells (DCs). The affinity of the interaction with p:MHCII plays a role in differentiation by mechanisms that are not completely understood. We found low-affinity TCRs biased mouse naive become follicular helper (Tfh) cells, whereas higher-affinity promoted formation Th1 or Th17 cells. explored basis for this phenomenon focusing IL-2R...
Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to quantitative one. However, biologists are adopting these experiments become more complex, researchers need additional expertise carry out this work rigorous reproducible manner. This Essay provides navigational guide for experimental aid understanding of sample preparation through image acquisition, analysis, data interpretation. We discuss the interconnectedness...
The HEALthy Brain and Child Development (HBCD) Study, a multi-site prospective longitudinal cohort study, will examine human brain, cognitive, behavioral, social, emotional development beginning prenatally planned through early childhood. acquisition of multimodal magnetic resonance-based brain data is central to the study's core protocol. However, application Magnetic Resonance Imaging (MRI) methods in this population complicated by technical challenges difficulties imaging life. Overcoming...
The HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, number proteins increases over several orders magnitude from a few at nucleation thousands completion. challenge in studying lies fact that current methods such as standard fluorescence or electron microscopy techniques cannot access all stages process cellular context. Here, we demonstrate an approach using super-resolution imaging permits quantitative morphological and...
Host immune response may predict the course of colorectal cancer. We examined survival 468 cancer patients associated with two tumor-infiltrating biomarkers, number cytotoxic T lymphocytes (CTLs), and activated CTLs, as reflected by cells expressing granzyme B (GZMB) in prospective Iowa Women's Health Study.
Abstract During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to non-specialist and care is still needed produce meaningful results. PALMsiever been developed provide a user-friendly means of visualizing, filtering analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms 3D visualization. incorporates main...
Synapse loss and dendritic damage correlate with cognitive decline in many neurodegenerative diseases, underlie neurodevelopmental disorders, are associated environmental drug-induced CNS toxicities. However, screening assays designed to measure of synaptic connections between live cells lacking. Here, we describe the design validation ASIA (automated imaging assay), an efficient approach label, image, analyze synapses neurons. Using viral transduction express fluorescent proteins that label...
The ample variety of labeling dyes and staining methods available in fluorescence microscopy has enabled biologists to advance the understanding living organisms at cellular molecular level. When two or more fluorescent are used same preparation, one dye is presence autofluorescence, separation emissions can become problematic. Various approaches have been recently proposed solve this problem. Among them, blind non-negative matrix factorization gaining interest since it requires little...
DNA nanotubes can cross blood-brain tumor barrier and enhance survival with no toxicity when used for the local delivery of DOX.
Bioimage analysis (BIA), a crucial discipline in biological research, overcomes the limitations of subjective microscopy through creation and application quantitative reproducible methods. The establishment dedicated BIA support within academic institutions is vital to improving research quality efficiency can significantly advance scientific discovery. However, lack training resources, limited career paths insufficient recognition contributions made by bioimage analysts prevent full...