A5073832866- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Cell Image Analysis Techniques
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Genomics and Chromatin Dynamics
- Forensic Fingerprint Detection Methods
- Advanced Neural Network Applications
- Hair Growth and Disorders
- Wound Healing and Treatments
- Phagocytosis and Immune Regulation
- Image Processing Techniques and Applications
- Video Surveillance and Tracking Methods
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Developmental Biology and Gene Regulation
- interferon and immune responses
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Cytokine Signaling Pathways and Interactions
- Nuclear Receptors and Signaling
Howard Hughes Medical Institute
2017-2024
Rockefeller University
2017-2024
Boston University
2021-2022
Genome Institute of Singapore
2011-2020
George Mason University
2015
National University of Singapore
2011-2015
Background & AimsThere are few in vitro models for studying the 3-dimensional interactions among different liver cell types during organogenesis or disease development. We aimed to generate hepatic organoids that comprise parenchymal and have structural features of liver, using human pluripotent stem cells.MethodsWe cultured H1 embryonic cells (WA-01, passage 27-40) induced (GM23338) with a series chemically defined serum-free media induce formation posterior foregut cells, which were...
Pathogen infection and tissue injury are universal insults that disrupt homeostasis. Innate immunity senses microbial infections induces cytokines/chemokines to activate resistance mechanisms. Here, we show that, in contrast most pathogen-induced cytokines, interleukin-24 (IL-24) is predominately induced by barrier epithelial progenitors after independent of microbiome or adaptive immunity. Moreover, Il24 ablation mice impedes not only epidermal proliferation re-epithelialization but also...
Stem cells remember Tissue stem sense their surroundings, and this perception influences subsequent fate function. Gonzales et al . observed that accumulate epigenetic memories of diverse environmental events (see the Perspective by Hoste). By wounding skin monitoring temporal steps involved in mobilizing hair follicle to repair epidermis, authors found bear original niche, migration, encounters with inflammation, adaptation new tasks. During homeostasis, immigrant are functionally...
Abstract Billions of cells are eliminated daily from our bodies 1–4 . Although macrophages and dendritic dedicated to migrating engulfing dying debris, many epithelial mesenchymal tissue can digest nearby apoptotic corpses How these non-motile, non-professional phagocytes sense eliminate while maintaining their normal functions is unclear. Here we explore the mechanisms that underlie multifunctionality by exploiting cyclical bouts regeneration degeneration during hair cycling. We show...
Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to quantitative one. However, biologists are adopting these experiments become more complex, researchers need additional expertise carry out this work rigorous reproducible manner. This Essay provides navigational guide for experimental aid understanding of sample preparation through image acquisition, analysis, data interpretation. We discuss the interconnectedness...
The distinct states of pluripotency in the pre- and post-implantation embryo can be captured vitro as naive primed pluripotent stem cell cultures, respectively. study application state remains hampered, particularly humans, partially due to current culture protocols relying on extraneous undefined factors such feeders. Here we performed a small-molecule screen identify compounds that facilitate chemically defined establishment maintenance human feeder-independent embryonic (FINE) cells....
The COVID-19 pandemic has increased the use of rapid antigen tests such as Abbott BinaxNOWTM Antigen Self-Test. In winter 2021-2022, omicron variant surge made it quickly apparent that although diagnostic (RDTs) are less sensitive than qRT-PCR, accessibility, ease use, and read-outs RDTs them a sought after often sold-out item at local suppliers. Here, we to qualify test for in our university testing program method rule-in positive or rule-out negative individuals when they seek care...
The COVID-19 pandemic has increased use of rapid diagnostic tests (RDTs). In winter 2021 to 2022, the Omicron variant surge made it apparent that although RDTs are less sensitive than quantitative reverse transcription-PCR (qRT-PCR), accessibility, ease use, and readouts them a sought after often sold-out item at local suppliers. Here, we qualify Abbott BinaxNOW RDT for in our university testing program as method rule positive or out negative individuals quickly priority qRT-PCR site. To...
Abstract In 2019, the first cases of SARS-CoV-2 were detected in Wuhan, China, and by early 2020 identified United States. infections increased US causing many states to implement stay-at-home orders additional safety precautions mitigate potential outbreaks. As policies changed throughout pandemic restrictions lifted, there was an increase demand for Covid-19 testing which costly, difficult obtain, or had long turn-around times. Some academic institutions, including Boston University,...
While distinct cell cycle structures have been known to correlate with pluripotent or differentiated states [1], there is no evidence on how the machinery directly contributes human embryonic stem (hESC) pluripotency. We established a determinant role of machineries state by demonstrating that specific perturbation S and G2 phases can prevent dissolution (PSD) [2]. Active mechanisms in these phases, such as DNA damage checkpoint Cyclin B1, promote To understand behind effect PSD pathways...
With the growth of urban centers worldwide, number vehicles in and around these areas has also increased. Traffic-related data plays an important role spatial planning, for example, optimizing road networks estimation or simulation air noise pollution. This information is as it reflects changes taking place us. Additionally, collected can be used a wide array applications including law enforcement, fleet management, supporting other analyses at varying scales. In this paper, we present...
ABSTRACT Pathogen infection and tissue injury are universal insults that disrupt homeostasis. Innate immunity senses microbial infections induces interferons (IFNs) to activate resistance mechanisms. Applying unbiased phylogenetic analysis, we show interleukin-24 (IL24) is among the closest evolutionary homologs IFN family shares a common ancestral origin. However, in contrast IFNs, IL24 induction occurs specifically barrier epithelial progenitors after independent of microbiome or adaptive...