A5000760969- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Industrial Vision Systems and Defect Detection
- Immune responses and vaccinations
- T-cell and B-cell Immunology
- Radiomics and Machine Learning in Medical Imaging
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Digital Imaging for Blood Diseases
- Macrophage Migration Inhibitory Factor
- Liver physiology and pathology
- Scheduling and Optimization Algorithms
- Immune cells in cancer
- Platelet Disorders and Treatments
- Influenza Virus Research Studies
- Biosensors and Analytical Detection
- Science, Research, and Medicine
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Biomedical and Engineering Education
Rockefeller University
2020-2025
Howard Hughes Medical Institute
2021-2024
National Institute of Allergy and Infectious Diseases
2019-2022
National Institutes of Health
2019-2022
Centre of Experimental Medicine of the Slovak Academy of Sciences
2014
The diverse composition of mammalian tissues poses challenges for understanding the cell-cell interactions required organ homeostasis and how spatial relationships are perturbed during disease. Existing methods such as single-cell genomics, lacking a context, traditional immunofluorescence, capturing only two to six molecular features, cannot resolve these issues. Imaging technologies have been developed address problems, but each possesses limitations that constrain widespread use. Here we...
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Stem cells remember Tissue stem sense their surroundings, and this perception influences subsequent fate function. Gonzales et al . observed that accumulate epigenetic memories of diverse environmental events (see the Perspective by Hoste). By wounding skin monitoring temporal steps involved in mobilizing hair follicle to repair epidermis, authors found bear original niche, migration, encounters with inflammation, adaptation new tasks. During homeostasis, immigrant are functionally...
Abstract Billions of cells are eliminated daily from our bodies 1–4 . Although macrophages and dendritic dedicated to migrating engulfing dying debris, many epithelial mesenchymal tissue can digest nearby apoptotic corpses How these non-motile, non-professional phagocytes sense eliminate while maintaining their normal functions is unclear. Here we explore the mechanisms that underlie multifunctionality by exploiting cyclical bouts regeneration degeneration during hair cycling. We show...
Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Cells encountering stressful situations activate the integrated stress response (ISR) pathway to limit protein synthesis and redirect translation better cope. The ISR has also been implicated in cancers, but redundancies stress-sensing kinases that trigger have posed hurdles dissecting physiological relevance. To overcome this challenge, we targeted regulatory node of these kinases, namely, S51 phosphorylation site eukaryotic initiation factor eIF2α genetically replaced with eIF2α-S51A mouse...
Abstract Maintaining tissue function while eliminating infected cells is fundamental to host defense. Innate inflammatory damage contributes lethal influenza and COVID-19, yet other than steroids, immunomodulatory drugs have modest effects. Among more 50 regimes tested in mouse infection, only the previously reported early depletion of neutrophils showed efficacy, suggesting that passes an tipping point which limiting innate immune alone cannot rescue physiological function. To re-balance...
Tissue resident macrophages and recruited monocyte-derived contribute to host defense but also play pathological roles in a diverse range of human diseases. Multiple macrophage phenotypes are often represented diseased tissue, we lack deep understanding the mechanisms that control diversification. Here use combination genetic, genomic, imaging approaches investigate origins epigenetic trajectories hepatic myeloid cells during diet-induced non-alcoholic steatohepatitis (NASH). The NASH diet...
Skin microbiota can be engineered to induce antitumor T cells.
This protocol allows users to image histology tissue using basic immunohistochemistry techniques in combination with EdU capture (using the Click-it reaction system). (5-ethynyl-2′-deoxyuridine) will S-phase cells thymidine analogue, allowing take a snap-shot of actively diving cells. In this assay modified analogue is efficiently incorporated into newly synthesized DNA and fluorescently labeled bright, photostable Alexa Fluor™ dye fast, highly-specific click reaction. fluorescent labeling...
Abstract Potentially auto-reactive T cells routinely escape thymic negative selection, but rarely elicit peripheral autoimmunity. We investigated the fate of these in murine secondary lymphoid organs using quantitative imaging approaches. During steady state, a small number seemingly CD4+ were detected along cell zone border. These displayed clear signs antigenic stimulation, specifically plasmalemma expression PD-1, absence foreign bacteria or host commensals. Remarkably, high densities...
Abstract Kupffer cells have specialized roles supporting the environment of liver during homeostasis and disease. However, key regulatory elements governing these behaviors are unknown. Using scRNA-seq, we found diversification recruitment additional macrophage subtypes nonalcoholic steatohepatitis (NASH). A significant source heterogeneity NASH was traced to Cx3cr1 expressing monocytes. Further, subsets were localized in distinct niches, suggesting environmental specification as a...