A5045255121- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- Digital Imaging for Blood Diseases
- Industrial Vision Systems and Defect Detection
- Scheduling and Optimization Algorithms
- Genetics, Bioinformatics, and Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Functional Brain Connectivity Studies
- Occupational and environmental lung diseases
- Complement system in diseases
- Neural and Behavioral Psychology Studies
- HER2/EGFR in Cancer Research
- Neural dynamics and brain function
- Microfluidic and Bio-sensing Technologies
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Biosensors and Analytical Detection
- Scientific Computing and Data Management
- AI in cancer detection
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Advanced Fluorescence Microscopy Techniques
National Institute of Allergy and Infectious Diseases
2020-2025
National Institutes of Health
2023-2025
Immune Regulation (United Kingdom)
2025
Vanderbilt University
2020-2024
Stony Brook University
2016-2020
Advanced Tissue (United States)
2020
The diverse composition of mammalian tissues poses challenges for understanding the cell-cell interactions required organ homeostasis and how spatial relationships are perturbed during disease. Existing methods such as single-cell genomics, lacking a context, traditional immunofluorescence, capturing only two to six molecular features, cannot resolve these issues. Imaging technologies have been developed address problems, but each possesses limitations that constrain widespread use. Here we...
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
The mesocortical dopamine system is comprised of midbrain neurons that predominantly innervate the medial prefrontal cortex (mPFC) and exert a powerful neuromodulatory influence over this region
Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Mesothelioma is an aggressive cancer of the serous membranes with poor prognosis despite combination therapy consisting surgery, radiotherapy, and platinum-based chemotherapy. Targeted therapies, including immunotherapies, have reported limited success, suggesting need for additional therapeutic targets. This study investigates a potential new target, gC1qR/HABP1/p32 (gC1qR), which overexpressed in all morphologic subtypes mesothelioma. gC1qR complement receptor that associated several...
The immunopathological functions associated with human C1q are still growing in terms of novelty, diversity, and pathologic relevance. It is, therefore, not surprising that is being recognized as an important molecular bridge between innate adaptive immunity. secret this functional turn, resides the elegant but complex structure molecule, which assembled from three distinct gene products: A, B, C, each has evolved a separate unique ancestral template. molecule made up 6A, 6B, 6C polypeptide...
Abstract There is robust experimental data, which show that C1q and gC1qR play a pro- as well anti-proliferative role in cancer. This opposing turn depends on whether each molecule expressed the cell surface or pericellular milieu. The aim of present studies was therefore to more closely examine functions soluble membrane forms using SkBr3 line model for breast Our results that: (1) In addition cC1qR, cells express C1q, these molecules appear be co-localized with other fibrinogen (FGN)...
Abstract The prognosis of acute myeloid leukemia (AML) has improved for younger patients, but it still remains grim patients over 65 years old with a likelihood death within year diagnosis. Here, we evaluated the effects novel anti-gC1qR monoclonal antibodies (mAbs) that were generated against intact U937 cells, which are used here as model AML. cell line is known to overexpress gC1qR, in turn serves not only proliferative molecular signal also provides platform generation angiogenic and...
Abstract The prognosis of acute myeloid leukemia (AML) has improved for younger patients, but it still remains grim patients over 65 years old with a likelihood death within year diagnosis. Here, we evaluated the effects novel anti-gC1qR monoclonal antibodies (mAbs) that were generated against intact U937 cells, which are used here as model AML. cell line is known to overexpress gC1qR, in turn serves not only proliferative molecular signal also provides platform generation angiogenic and...
Abstract The diverse composition of mammalian tissues poses challenges for understanding the cell-cell interactions required organ homeostasis and how spatial relationships are perturbed during disease. Existing methods such as single-cell genomics, lacking a context, traditional immunofluorescence, capturing only 2-6 molecular features, cannot resolve these issues. Imaging technologies have been developed to address problems, but each possesses limitations that constrain widespread use....