A5034824433- Blood properties and coagulation
- Platelet Disorders and Treatments
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Dermatological and COVID-19 studies
- Polymer Surface Interaction Studies
- Clinical Laboratory Practices and Quality Control
- Blood groups and transfusion
- Antiplatelet Therapy and Cardiovascular Diseases
- Protein Interaction Studies and Fluorescence Analysis
- Streptococcal Infections and Treatments
- Hemostasis and retained surgical items
- Rheology and Fluid Dynamics Studies
- Proteins in Food Systems
- Atrial Fibrillation Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Pharmacology and Obesity Treatment
- Mesenchymal stem cell research
- Complementary and Alternative Medicine Studies
- Nerve injury and regeneration
- Poisoning and overdose treatments
Stony Brook University
2010-2024
Stony Brook School
1996-2021
Stony Brook University Hospital
1992-2020
State University of New York
1993-2020
Stony Brook Medicine
1998-2017
University of Pennsylvania
2016
University of Pittsburgh
2016
West Virginia University
2016
University Medical Center
2009
Goethe University Frankfurt
2009
Scar formation in the nervous system begins within hours after traumatic injury and is characterized primarily by reactive astrocytes depositing proteoglycans that inhibit regeneration. A fundamental question CNS repair has been identity of initial molecular mediator triggers glial scar formation. Here we show blood protein fibrinogen, which leaks into immediately blood–brain barrier (BBB) disruption or vascular damage, serves as an early signal for induction via TGF-β/Smad signaling...
The heterogeneity of human plasma fibrinogen manifested as differences in solubility reflects the presence early catabolic intermediates, which are more soluble, have a longer thrombin-clotting time, and lower molecular weight than parent material from they formed. In this study S-sulfo subunits fractions low (I-4) high (I-8 I-9) were compared. Separation Aα, Bβ, γ chains was achieved by gradient elution chromatography on CM-cellulose. tryptic peptide maps characteristic for each type chain....
Abstract In an effort to determine the essential structural features of human fibrinogen, plasmic hydrolysis was carried out and degradation products were identified analyzed. agreement with previous work, initial attack shown occur in Aα chains release portions their COOH-terminal regions (designated /α terminology developed describe present findings), whereas simultaneous, but slower, occurs NH2-terminal region Bβ/ chains) results removal remnants containing peptide B. These changes, plus...
Abstract Human plasma fibrinogen Subfractions I-8 and I-9 have previously been shown to consist of catabolic intermediates characterized by the lack COOH-terminal portions Aα chains. The present study was undertaken determine structural relationships between these catabolites core derivatives formed in vitro action plasmin. First, fractions were compared with Subfraction I-4, which is representative native fibrinogen, polyacrylamide gel electrophoresis unreduced samples sodium dodecyl...
Abstract Neural stem/progenitor cells (NSPCs) originating from the subventricular zone (SVZ) contribute to brain repair during CNS disease. The microenvironment within SVZ stem cell niche controls NSPC fate. However, extracellular factors that trigger astrogliogenesis over neurogenesis disease are unclear. Here, we show blood-derived fibrinogen is enriched in following distant cortical injury mice. Fibrinogen inhibited neuronal differentiation and hippocampal NSPCs while promoting...
The molecular imprinting technique has tremendous applications in artificial enzymes, bioseparation, and sensor devices. In this study, a novel (MI) biosensor platform was developed for the detection of broad range biomolecules with different sizes. Previously method been applied to 2D imprinting, where height self-assembled monolayer (SAM) around 2 nm limited maximum dimensions molecule that can be imprinted create template-shaped cavities. order match size molecules SAM, we propose model...
ADVERTISEMENT RETURN TO ISSUEPREVArticleAlbumin modulates lateral assembly of fibrin polymers: evidence enhanced fine fibril formation and unique synergism with fibrinogenDennis K. Galanakis, Bernard P. Lane, Sanford R. SimonCite this: Biochemistry 1987, 26, 8, 2389–2400Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/bi00382a046https://doi.org/10.1021/bi00382a046research-articleACS...
Wound healing is a complex process initiated by the formation of fibrin fibers and endothelialization. Normally, this triggered in wound thrombin cleavage fibrinopeptides on fibrinogen molecules, which allows them to self spontaneously-assemble into large that provide support structure clot promote healing. We have found fibrous structures can also form without most polymer or metal surfaces, including those commonly used for stents. show relatively hydrophobic E D regions molecule are...
High-resolution atomic force microscopy imaging reveals the role of fibrinogen αC regions in early stages fibrin self-assembly.
Abstract Bradykinin (BK) is one of the most potent vasodilator agonists known and belongs to kinin family proinflammatory peptides. BK induces its activity via two G protein–coupled receptors: receptor 1 (B1R) 2. Although 2 constitutively expressed on endothelial cells (ECs), B1R induced by IL-1β. The C1q receptor, for globular heads (gC1qR), which plays a role in generation, activated ECs also secreted as soluble gC1qR (sgC1qR). Because sgC1qR can bind ECs, we hypothesized that it may serve...
We studied the hydrodynamic behavior of fibrinogen, a blood plasma protein involved in clotting, broad 0.3-60 mg/mL range concentration and 5-42 °C temperature using pulsed-field gradient 1H NMR-diffusometry. Arrhenius plots revealed activation energy for fibrinogen diffusion Ed = 21.3 kJ/mol at 1.4 28.4 38 mg/mL. found dramatic slowdown self-diffusion with beginning 1.7-3.4 mg/mL, which deviated from standard hard-particle behavior, suggesting remarkable intermolecular entanglement. This...
Hereditary dysfibrinogenemia is a rare clotting disorder, which results from mutations in at least one of the three fibrinogen genes. We examined frequency hemostatic clinical and laboratory anomalies presentation 37 probands 12 unrelated families with five different defects (Aalpha R16C, gamma A357T, gamma318-319 del, M310T, Aalpha R16S), among. The median age was 51 years (11-86 years). Among 62% who were women (13%) had experienced or more spontaneous abortion. More than half undue...
Thromboelastography (TEG), a widely used clinical point of care coagulation test, is poorly understood. To investigate its fibrin determinants we normal and variant fibrinogen isolates.We focused mainly on the TEG maximum signal amplitude (MA), shear modulus clot stiffness indicator. Isolates included des-αC, cord, abnormal congenital variants with amino acid substitutions or deletions that impaired polymerization. Heterophenotypic isolates were from cryoprecipitate-depleted plasma owing to...
Assessed by high performance liquid chromatographic and amino acid sequence determinations, approximately one half (n = 4) of A peptide in fibrinogen Stony Brook (phi SB) contained the alpha 16Arg----Cys substitution. To examine its functional behavior, mutant molecule-rich soluble subfractions that partly or fully lacked their normal were obtained from cryoprecipitates incoagulable material, respectively. Such consistently induced a more pronounced decrease 3) turbidity polymerizing fibrin...
Dysfibrinogenemia is caused by a variety of structural abnormalities in the fibrinogen molecule, which results tendency for bleeding and thrombosis as well obstetric complications. The complications dysfibrinogenemia include first-trimester pregnancy loss, hemorrhage, placental abruption, thrombosis. We conducted retrospective study four cases dysfibrinogenemic patients from one family (fibrinogen Frankfurt III) with history recurrent loss who were treated concentrates. One patient had three...
Abstract Background Chamomile administration may have desirable effects in the perioperative setting. Current practice, however, discourages chamomile use due to a theoretical increase bleeding. Therefore, we evaluated if acutely (within 4 h of ingestion) prolongs coagulation assays. Methods Eight healthy volunteers were randomized receive 2 interventions crossover design: (a) single dose extract capsule (500 mg) and (b) tea (3 g 150 mL water). Interventions separated at least 3 days apart...
Gas-phase, water-soluble components of cigarette smoke cause delayed fibrin self-assembly and prevent cross-linking by inactivation factor XIIIa (plasma transglutaminase). These anticoagulant properties are demonstrable in plasma, suggesting they play a role the pathophysiology smoking.
The accessibility of activated GPIIb/IIIa receptors on the luminal surface platelets adherent to damaged blood vessels or atherosclerotic plaques is likely play a crucial role in subsequent platelet recruitment. To define better factors involved this process, we developed functional assay assess presence activated, receptors, based their ability bind erythrocytes containing high density covalently coupled RGD-containing peptides (thromboerythrocytes). Platelets readily adhered wells coated...