A5023762221- Epigenetics and DNA Methylation
- Blood properties and coagulation
- Workplace Health and Well-being
- Cancer-related gene regulation
- Fungal Infections and Studies
- Histone Deacetylase Inhibitors Research
- Chronic Lymphocytic Leukemia Research
- Respiratory viral infections research
- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Platelet Disorders and Treatments
- Chronic Myeloid Leukemia Treatments
- RNA modifications and cancer
- Influenza Virus Research Studies
- Molecular Biology Techniques and Applications
- Data-Driven Disease Surveillance
- Bone health and treatments
- Stress and Burnout Research
- Occupational Health and Burnout
- Plant Disease Resistance and Genetics
- Connective tissue disorders research
- Cancer, Lipids, and Metabolism
- Folate and B Vitamins Research
- Venous Thromboembolism Diagnosis and Management
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Northwell Health
2012-2024
Ludwig Boltzmann Institute of Osteology
2009-2023
Hanusch Hospital
2009-2023
Stony Brook University
1998-2022
Technische Universität Dresden
2008-2022
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2018-2019
Stony Brook University Hospital
1998-2018
North Shore University Hospital
2010-2018
Carl Gustav Carus-Institut
2017
Hofstra University
2017
Elevated homocysteine (Hcys) serum levels represent a risk factor for several chronic pathologies, including cardiovascular disease, atherosclerosis, and renal failure, affect bone development, quality, homeostasis. Hcys influences the formation of stable matrix directly through inhibition collagen cross-linking enzyme lysyl oxidase (Lox) and, as we have shown recently, by repressing its mRNA expression. The aim this study was to investigate mechanisms involved in process. Through evaluation...
Artificial induction of active DNA demethylation appears to be a possible and useful strategy in molecular biology research therapy development. Dimethyl sulfoxide (DMSO) was shown cause phenotypic changes embryonic stem cells altering the genome-wide methylation profiles. Here we report that DMSO increases global gene-specific hydroxymethylation levels pre-osteoblastic MC3T3-E1 cells. After 1 day, increased expression genes involved (TET) nucleotide excision repair (GADD45) decreased...
Stem cell lines that faithfully maintain the regional identity and developmental potency of progenitors in human brain would create new opportunities neurobiology provide a resource for generating specialized neurons. However, to date, neural progenitor cultures derived from have either been short-lived or exhibit restricted, predominantly glial, differentiation capacity. Pluripotent stem cells are an alternative source, but ascertain definitively fidelity types generated solely <i>in...
We isolated a recombinant phage from Cryptococcus neoformans genomic library that contains member of dispersed family repetitive DNA elements. This clone, CNRE-1, hybridized to at least seven chromosomes in C. on the basis pulsed-field gel analysis. Hybridization CNRE-1 restriction digests confirmed there are multiple copies this element and fragment length polymorphisms present strains different serotypes neoformans. The utility probe as an epidemiologic marker was determined by testing...
Abstract
High degrees of premature retirement among teachers warrant investigating the occupational burden and mental health status this profession. A sample 1074 German participated in study. Two samples general population (N = 824 N 792) were used as comparison groups. Work distress was assessed with Effort–Reward-Imbalance questionnaire, problems measured General Health Questionnaire (GHQ-12). Teachers reported more effort–reward imbalance (M 0.64) compared 0.57), they perceived (GHQ: M 12.1) than...
Health care and public health professionals rely on accurate, real-time monitoring of infectious diseases for outbreak preparedness response. Early detection outbreaks is improved by systems that are comprehensive specific with respect to the pathogen but rapid in reporting data. It has proven difficult implement these requirements a large scale while maintaining patient privacy.The aim this study was demonstrate automated export, aggregation, analysis disease diagnostic test results from...
Abstract This case-control study examined the prevalence of a prothrombin gene mutation in 3′-untranslated region (UTR) first reported by Poort et al Dutch subjects with history venous thrombosis and matched control without thrombosis. We tested hypothesis that presence 3′UTR would convey higher risk or arterial therefore be found higher-than-normal percentage Our included 100 subjects: 50 (21 29 thrombosis, who had been recruited from an anticoagulation clinic) DNA these was analyzed...
Thyroid hormones (T3, T4) have a broad range of effects on bone, however, its role in determining the quality bone matrix is poorly understood. In-vitro, immortalized mouse osteoblast-like cell line MC3T3-E1 forms tissue like structure, consisting several layers, whose formation affected by T3 significantly. In this culture system, we investigated multiplication, collagen synthesis, expression genes related to cross-linking process and cross-links. compared controls modulated up-regulated...
Hereditary dysfibrinogenemia is a rare clotting disorder, which results from mutations in at least one of the three fibrinogen genes. We examined frequency hemostatic clinical and laboratory anomalies presentation 37 probands 12 unrelated families with five different defects (Aalpha R16C, gamma A357T, gamma318-319 del, M310T, Aalpha R16S), among. The median age was 51 years (11-86 years). Among 62% who were women (13%) had experienced or more spontaneous abortion. More than half undue...
Hyperhomocysteinemia is a risk factor for osteoporotic fractures. Homocysteine (Hcys) inhibits collagen cross-linking and consequently decreases bone extracellular matrix (ECM) quality. Serum amyloid A (A-SAA), an acute-phase protein family, plays important role in chronic inflammatory diseases up-regulates MMP13, which development remodeling. Here, we investigate the effect of Hcys on expression SAA3, member A-SAA gene osteoblasts characterizing underlying mechanisms possible consequences...
Serum amyloid A (A-SAA/Saa3) was shown before to affect osteoblastic metabolism. Here, using RT-quantitative PCR and/or immunoblotting, we show that expression of mouse Saa3 and human SAA1 SAA2 positively correlates with increased cellular maturation toward the osteocyte phenotype. Expression is not detected in C3H10T1/2 embryonic fibroblasts but successively higher preosteoblastic MC3T3-E1 cells, late MLO-A5 MLO-Y4 osteocytes, consistent findings primary bone cells from newborn calvaria....
Cryptococcus neoformans isolates from eight patients with cryptococcal infection were previously assigned into three groups on the basis of repetitive DNA probe (CNRE-1) restriction fragment length polymorphisms. These accounted for a disproportionate number recent clinical in New York City. To further examine genetic relatedness within and across CNRE-1 groups, sequence 779-base URA5 gene each strain was amplified sequenced. The nucleotide differences occurred third codon position or...
There is growing evidence that aminobisphosphonates like ibandronate show anticancer activity by an unknown mechanism. Biochemically, they prevent posttranslational isoprenylation of small GTPases, thus inhibiting their activity. In tumor cells, activated RAS-GTPase, the founding member gene family, down-regulates expression pro-apoptotic FAS via epigenetic DNA-methylation DNMT1. We compared treatment in neoplastic human U-2 osteosarcoma and mouse CCL-51 breast cancer cells as well...
Abstract Mospd1 codes for a small protein with unknown physiological function, which is part of family genes, including Mospd2 and Mospd3 , defined by the presence major sperm domain two transmembrane domains. This work characterizes gene, intracellular location its expression in different mouse tissues mesenchymal cell lines during differentiation. The role cellular differentiation was studied siRNA knockdown experiments osteoblastic MC3T3‐E1 cells. Transfection targeted cDNA show MOSPD1...
An SP6/mouse insulin RNA precursor containing two exons and one intron can be spliced in a partially purified nuclear extract isolated from MOPC-315 mouse myeloma cells. We have detected the putative splicing intermediate (intron-3'exon) lariat form, excised mRNA product. The vitro reaction of gel-purified precursors requires ATP Mg 2plus; was accompanied by formation 60-40S ribonucleoprotein complex. 60S complex ATP. At least Sm snRNPs Ul U2 RNAs are components assemble those occurs early...