A5040921455- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Infective Endocarditis Diagnosis and Management
- Respiratory viral infections research
- Antibiotic Use and Resistance
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Antifungal resistance and susceptibility
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Nosocomial Infections in ICU
- Clinical Nutrition and Gastroenterology
- Fungal Infections and Studies
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Microscopic Colitis
Wayne State University
2016-2025
Detroit Medical Center
2016-2025
DMC University Laboratories
2013-2024
Children's Hospital of Michigan
2017-2024
Michigan United
2024
Central Michigan University
2023
Museum of Heilongjiang Province
2021
Sparrow Hospital
2021
Johns Hopkins University
2019
Children's Mercy Hospital
2018
Rapid diagnosis and treatment of infectious meningitis encephalitis are critical to minimize morbidity mortality. Comprehensive testing cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, molecular methods, paired with chemical cellular analyses. These methods may lack sensitivity or specificity, can take several days, require significant volume for complete analysis. The FilmArray Meningitis/Encephalitis (ME) Panel is a multiplexed in vitro diagnostic test the...
ABSTRACT Sepsis is a major cause of morbidity, mortality, and increased medical expense. Rapid diagnosis improves outcomes reduces costs. The FilmArray blood culture identification panel (BioFire Diagnostics LLC, Salt Lake City, UT), highly multiplexed PCR assay, can identify 24 etiologic agents sepsis (8 Gram-positive, 11 Gram-negative, 5 yeast species) three antimicrobial resistance genes ( mecA , vanA / B bla KPC ) from positive bottles. It provides results in about 1 h with 2 min for...
Carbapenem-resistant Klebsiella pneumoniae has spread worldwide and throughout the United States. Colistin is used extensively to treat infections with this organism. We describe a cluster of colistin-resistant, carbapenem-resistant K. infection cases involving three institutions in Detroit, MI. A five was identified at Detroit Medical Center (DMC) from 27 July 22 August 2009. Epidemiologic data were collected, transmission opportunities analyzed. Isolates genotyped by using pulsed-field gel...
The intestinal microbiome represents a complex network of microbes that are important for human health and preventing pathogen invasion. Studies examine differences in microbial communities across individuals with without enteric infections useful identifying support or impede health.16S rRNA gene sequencing was conducted on stool DNA from patients (n = 200) 75 healthy family members to identify community composition. Stools 13 were also examined post-infection better understand how recover....
Given the limited information on Clostridium difficile infection (CDI) during hematopoietic stem cell transplantation (HSCT), we examined recent epidemiology of CDI in HSCT recipients at our institution. During two-yr retrospective study period (2005-2006), 361 transplants were performed: 60% allogeneic and 40% autologous. Among all hospitalized patients a non-outbreak setting, rates ninefold higher than those general 1.4-fold with cancer (24.0 vs. 2.6 16.8/10,000 patient-days respectively)....
ABSTRACT We evaluated the ability of four commercial MIC testing systems (MicroScan, Vitek 2, Phoenix, and Etest) to detect vancomycin values ≤1 ≥2 in 200 methicillin-resistant Staphylococcus aureus (MRSA) strains compared Clinical Laboratory Standards Institute broth microdilution (BMD) reference methods. Compared BMD method, absolute agreement (0 ± dilution) was highest for Phoenix system (66.2%) MicroScan turbidity method (61.8%), followed by 2 (54.3%). The Etest produced 1 dilutions...
Tigecycline is one of the few remaining therapeutic options for extensively drug-resistant (XDR) Gram-negative bacilli (GNB). MICs tigecycline to Acinetobacter baumannii have been reported be elevated when determined by Etest compared determinations broth microdilution (BMD) method. The study aim was compare susceptibility GNB four different testing methods. were collected from six health care systems (25 hospitals) in southeast Michigan January 2010 September 2011. among A. baumannii,...
Highlights•We study Clostridium difficile colonization in hematopoietic stem cell transplantation patients.•Colonization with C. was common this population.•Colonization toxigenic predictive of infection.•Colonization nontoxigenic decreases risk infection.AbstractClostridium is a leading cause infectious diarrhea transplant (HSCT) recipients. Asymptomatic the gastrointestinal tract occurs before development infection (CDI). This prospective examines rates, factors, and outcomes strains HSCT...
FilmArray Meningitis/Encephalitis (ME) polymerase chain reaction (PCR) panel was tested on 62 cerebrospinal fluid (CSF) samples from young infants (0-3 months) with suspected meningitis and compared CSF cultures. Twelve 9 were positive by ME PCR (10 Group B Streptococcus (GBS) 2 Escherichia coli) of which only 5 culture. The 7 that obtained who had received prior antibiotic treatment. can be a useful tool in the rapid diagnosis bacterial pretreated infants.
Background Hospitalization of patients infected with the severe acute respiratory syndrome virus 2 (SARS-CoV-2) have remained considerable worldwide. Patients often develop complications and high mortality rates. The cycle threshold (Ct) value derived from nasopharyngeal swab samples using real time polymerase chain reaction (RT-PCR) may be a useful prognostic marker in hospitalized SARS-CoV-2 infection, however, its role predicting course pandemic has not been evaluated thus far. Methods We...
A phenotypic and genotypic analysis of Acinetobacter baumannii was conducted from 2003 to 2008 in Detroit, MI. The incidence A. increased 1.7 3.7/1,000 patient days during the study period. Susceptibility ampicillin-sulbactam imipenem decreased approximately 90% 40%. Genotyping revealed polyclonality, suggesting either emergence multiple resistant strains or spread a common genetic element. sharp rise mandates major multidisciplinary interventions optimize management this multidrug-resistant...
Background. Carbapenem-resistant Enterobacteriaceae (CRE) are rapidly emerging in hospitals the United States and posing a significant threat. To better understand transmission dynamics acquisition of resistant strains, thorough analysis epidemiologic molecular characteristics was performed. Methods. CRE isolated at Detroit Medical Center were analyzed from September 2008 to 2009. bla KPC genes investigated by polymerase chain reaction (PCR), repetitive extragenic palindromic PCR (rep-PCR)...
Weissella confusa is found in fermented foods and has been suggested as a probiotic, but also causes sepsis other serious infections humans animals. The incidence of human underestimated partly due to confusion with viridans streptococci difficulty making definitive identification, even if the organism recognized belong another genus, owing inability commercial systems identify it. We report our experiences identifying W. isolated from two immune-compromised patients, both whom developed...
Rapid diagnostic tests (RDTs) have revolutionized the management of Gram-negative bacteremia by allowing antimicrobial stewardship teams ability to escalate therapy and improve patient outcomes through timely organism identification detection certain resistance determinants. However, given complex nature resistance, are left without clear direction for how respond when determinants absent, as safety de-escalation in this setting is unknown. The primary purpose analysis was determine negative...
Health care and public health professionals rely on accurate, real-time monitoring of infectious diseases for outbreak preparedness response. Early detection outbreaks is improved by systems that are comprehensive specific with respect to the pathogen but rapid in reporting data. It has proven difficult implement these requirements a large scale while maintaining patient privacy.The aim this study was demonstrate automated export, aggregation, analysis disease diagnostic test results from...
Campylobacter jejuni is a zoonotic pathogen and the most common bacterial cause of human gastroenteritis worldwide. With increase antibiotic resistance to fluoroquinolones macrolides, drugs choice for treatment, C. was recently classified as serious antimicrobial resistant threat. Here, we characterized 94 isolates collected from patients at four Michigan hospitals in 2011 2012 determine frequency association with phylogenetic lineages. The prevalence (19.1%) macrolides (2.1%) this subset...
We report our clinical and laboratory experience treating a 50-year-old patient who was critically ill with extensively drug-resistant Acinetobacter baumannii necrotizing pneumonia complicated by empyema in Detroit, Michigan. A precision medicine approach using whole-genome sequencing, susceptibility testing, synergy analysis guided the selection of rational combination antimicrobial therapy.
Background: We noted a recent increase in cases of urinary tract infection due to community-acquired ESBL-producing Escherichia coli children treated at our institution. Risk factors E. the USA remain unclear.Methods: A single center retrospective case control study UTI CA-ESBL-producing during 5-year period (2012–2016). Control with non-ESBL-producing were matched by age, gender and year infection.Results: total 111 patients E 103 controls included. The proportion ranged from 7% 15% all...
Bacteremia can progress to septic shock and death without appropriate medical intervention. Increasing evidence supports the role of molecular diagnostic panels in reducing clinical impact these infections through rapid identification infecting organism associated antimicrobial resistance genes. We report results a multicenter study assessing performance GenMark Dx ePlex investigational-use-only blood culture Gram-negative panel (BCID-GN), assay for detection bloodstream pathogens positive...
We investigated the possibility of loading PMMA bone cement with antimicrobial nanostructured AgNbO3 particles to counter biofilm formation at cement-tissue interface. found that a formulation containing (1–4)% showed high antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa while not showing any toxicity THP1 human cell lines. In addition, did impact mechanical properties cement. The results thus obtained illustrate potential approach...