A5078132502- RNA modifications and cancer
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- Immune Cell Function and Interaction
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune responses and vaccinations
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Plant Virus Research Studies
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Valve Diseases and Treatments
- Gut microbiota and health
- Congenital gastrointestinal and neural anomalies
- T-cell and B-cell Immunology
- Scientific Computing and Data Management
National Institute of Allergy and Infectious Diseases
2023-2025
National Institutes of Health
2023-2025
Immune Regulation (United Kingdom)
2025
ShanghaiTech University
2017-2020
Shanghai Institutes for Biological Sciences
2017-2018
Chinese Academy of Sciences
2017-2018
Center for Excellence in Molecular Cell Science
2017-2018
University of Chinese Academy of Sciences
2017-2018
Abstract The intestinal barrier is mainly formed by a monolayer of epithelial cells, which forms physical to protect the gut tissues from external insults and provides microenvironment for commensal bacteria colonize while ensuring immune tolerance. Moreover, various cells are known significantly contribute function either directly interacting with or producing mediators. Fulfilling this mucosal homeostasis requires not only intrinsic regulation (IECs) but also constant communication...
Significance Recent success of T cell-based cancer immunotherapies highlights the importance further understanding molecular mechanisms in regulation cell responsiveness. Here, we performed a genome-wide CRISPR screen to identify genes that regulate activation upon anti-T receptor (TCR) stimulation. Our confirmed many known regulators proximal signaling. Moreover, identified previously uncharacterized gene named FAM49B, which acts as negative regulator activation. study suggests screening is...
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Abstract Sepsis‐induced immunosuppression is related to increased susceptibility secondary infections and death. Lung the most vulnerable target organ in sepsis, but understanding of pulmonary state still limited. Here, single‐cell RNA sequencing bronchoalveolar lavage fluid (BALF) performed map landscape immune cells, revealing a neutrophil‐driven immunosuppressive program lungs patients with sepsis. Although genes are upregulated different only neutrophils dramatically increase BALF phase...
Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.
The immune evasion is one major challenge for cancer immunotherapy. Despite considerable advancements in checkpoint blockade (ICB) therapies the advanced non-small cell lung (NSCLC) patients, only a minority of patients receive long-term survival benefit. Here, this work demonstrates that lysine methyltransferase 5C (KMT5C) crucial promoter NSCLC progression and evasion. This first observes upregulation KMT5C correlated with poor patient prognosis. Notably, knockdown cells suppress tumor...
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Increased differentiation or activity of osteoclasts is the key pathogenic factor postmenopausal osteoporosis (PMOP). N4‐acetylcytidine (ac4C) modification, catalyzed by Nat10, a novel posttranscriptional mRNA modification related to many diseases. However, its impact on regulating osteoclast activation in PMOP remains uncertain. Here, we initially observed that Nat10-mediated ac4C positively correlates with monocytes and low bone mass PMOP. The specific knockout Nat10 remodelin, inhibitor,...
Abstract Gasdermin (GSDM) family proteins mediate inflammatory cell pyroptosis and exert critical contributions to the pathogenesis of gastrointestinal cancers, infections, gut mucosal inflammation. C (GSDMC) is overexpressed in human colorectal cancer (CRC); however, molecular mechanisms underlying GSDMC regulation CRC tumorigenesis are largely elusive. Here, it found that both expression activation significantly elevated mouse tissues. Gsdmc2/3/4 deficiency attenuates tumor progression...
Stem-like progenitors are a critical subset of cytotoxic T cells that self-renew and give rise to expanded populations effector for successful checkpoint blockade immunotherapy. Emerging evidence suggests the tumor-draining lymph nodes can support continuous generation these stem-like replenish tumor sites act as source populations, underlining importance understanding what factors promote maintain activated in state. Using advanced 3D multiplex immunofluorescence imaging, here we identified...
T cell receptor (TCR) engagement causes a global cellular response that entrains signaling pathways, cycle regulation, and death. The molecular regulation of mRNA translation in these processes is poorly understood. Using whole-genome CRISPR screen for regulators CD95 (FAS/APO-1)-mediated death, we identified AMBRA1, protein previously studied its roles autophagy, E3 ubiquitin ligase activity, cyclin regulation. cells lacking AMBRA1 resisted FAS-mediated death by down-regulating FAS...
<h3>Background</h3> Stem-like CD8+ T cells constitute a subset of activated cytotoxic with high proliferative potential critical for successful checkpoint immunotherapy.<sup>1</sup> Emerging evidence suggests that tumor-draining lymph nodes (tdLN) can serve as reservoirs support the generation TCF-1+ stem-like continuously replenish tumor sites to sustain ongoing anti-tumor activity and responsiveness therapy.<sup>2 3</sup> However, how retain their stemness in highly antigenic inflammatory...