A5103233599- CAR-T cell therapy research
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- Immune cells in cancer
- Enzyme Structure and Function
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Lymphoma Diagnosis and Treatment
- Heterotopic Ossification and Related Conditions
- Ultrasound and Hyperthermia Applications
- Urological Disorders and Treatments
- RNA Research and Splicing
- Plant Reproductive Biology
- Autophagy in Disease and Therapy
- Nanowire Synthesis and Applications
- Educational Technology and Assessment
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Prostate Cancer Diagnosis and Treatment
- Advanced Computational Techniques and Applications
Southern Medical University
2024-2025
Nanfang Hospital
2025
Daping Hospital
2018-2024
Xinqiao Hospital
2016-2024
Army Medical University
2016-2024
Tsinghua University
2016-2023
Tokyo Medical and Dental University
2021
Center for Life Sciences
2018
Beijing Forestry University
2016
Huazhong Agricultural University
2013
Abstract Chimeric antigen receptor (CAR)‐T cell immunotherapy is approved in the treatment of hematological malignancies, but remains far from satisfactory solid tumor due to inadequate intra‐tumor CAR‐T infiltration. Herein, an injectable supramolecular hydrogel system, based on self‐assembly between cationic polymer mPEG‐PCL‐PEI (PPP) conjugated with T targeting anti‐CD3e f(ab')2 fragment and α‐cyclodextrin (α‐CD), designed load plasmid CAR (pCAR) a specific CD2 promoter, which...
Various methods for editing specific sites in the Escherichia coli chromosome are available, and gene-size (∼1 kb) integration into a single site or to introduce deletions, short insertions point mutations multiple can be conducted period of time. However, method rapidly integrating sequences different has not been developed yet. Here, we describe plasmid system that makes it possible simultaneously insert genes loci E. genome without need chromosomal markers. The uses CRISPR-Cas12a...
Folate receptor alpha (αFR) is overexpressed in 90% of ovarian cancers, one the most lethal gynecologic cancers. Recent studies have suggested that natural killer (NK) cells may be better chimeric antigen (CAR) drivers because their favorable innate characteristics, such as directly recognizing and killing tumor cells. However, therapeutic effects CAR-engineered NK targeting αFR cancer not been reported. In this research, 3 generations anti-αFR CAR were constructed, namely αFR-ζ (first...
Halomonas bluephagenesis is a halophilic bacterium capable of efficiently producing polyhydroxyalkanoates and other valuable chemicals through high salinity open fermentation, offering an appealing platform for next-generation industrial biotechnology. Various techniques have been developed to engineer bluephagenesis, each with its inherent shortcomings. Genome editing methods often entail complex time-consuming processes, while flexible expression systems relying on plasmids necessitate the...
Increased differentiation or activity of osteoclasts is the key pathogenic factor postmenopausal osteoporosis (PMOP). N4‐acetylcytidine (ac4C) modification, catalyzed by Nat10, a novel posttranscriptional mRNA modification related to many diseases. However, its impact on regulating osteoclast activation in PMOP remains uncertain. Here, we initially observed that Nat10-mediated ac4C positively correlates with monocytes and low bone mass PMOP. The specific knockout Nat10 remodelin, inhibitor,...
Targeted therapy for cancer is a research area of great interest, and magnetic nanoparticles (MNPs) show potential as targeted carriers therapeutics. One important class biomarkers microRNAs (miRNAs), which play significant role in tumor initiation progression. In this study, cascade recognition system containing multiple plasmids, including Tet activator, lacI repressor gene driven by the TetOn promoter, reporter repressed influenced endogenous miRNAs, was used to recognize cells that...
Basal elements in archaeal promoters, except for putative initiator encompassing transcription start sites, are well characterized. Here, we employed the Sulfolobus araS promoter as a model to study function of element (Inr) archaea. We have provided evidence presence third core element, Inr, whose action depends on TATA box and TFB recognition (BRE). Substitution mutations Inr did not alter location site. Using systematic mutagenesis, most functional was defined +1 GAGAMK +6 (where M is A/C...
Sepsis, which is characterized by multiple organ dysfunctions as a result of an unbalanced host-inflammatory response to pathogens, potentially life-threatening condition and major cause death in the intensive care units (ICUs). However, effective treatment or intervention prevent sepsis-associated lethality still lacking. Human umbilical cord mesenchymal stem cell (hUC-MSC) transplantation has been shown have potent immunomodulatory properties improve tissue repair yet lacks direct...
Abstract Chronic bacterial prostatitis (CBP) is an intractable disease. Although bone marrow mesenchymal stem cells (BMMSCs) are able to regulate inflammation in CBP, the effect of microbubble-mediated ultrasound- induced accumulation BMMSCs on CBP remains unclear. To address this gap, a model was established SD rats, which were then treated with alone (BMMSC group), ultrasound (ultrasound (MMUS group) and compared healthy control group. A therapeutic-ultrasound apparatus used treat prostate...
Herbaceous peony (Paeonia lactiflora) is a globally important ornamental plant. Spontaneous floral mutations occur frequently during cultivation, and are selected as way to release new cultivars, but the underlying evolutionary developmental genetics remain largely elusive. Here, we investigated collection of spontaneous corolla mutational plants (SCMPs) whose other organs were virtually unaffected. Unlike in normal (NPs) that withered soon after fertilization, transformed (petals) SCMPs was...
To elucidate the underlying mechanism by which proliferation and migration abilities of human umbilical cord mesenchymal stem cells (hUC-MSCs) determine their therapeutic efficacy in rheumatoid arthritis treatment.
Immune paralysis is a protracted state of immune suppression following the early/acute inflammatory phase sepsis. CD11b+ Gr-1+ cells induced during sepsis are heterogeneous myeloid-derived (MDCs). This study investigated contribution MDCs to paralysis. Treatment mice with zymosan (ZM) marked increase in total number splenocytes an proportion Gr-1hi and decrease T on day 7; levels these eventually return similar those control 21. T-cell activation gamma interferon (IFN-γ) expression by CD8+...
Soft tissue trauma can cause immune system disturbance and neuropathological invasion, resulting in heterotopic ossification (HO) due to aberrant chondrogenic differentiation of mesenchymal stem cells (MSCs). However, the molecular mechanisms behind interaction between nervous systems promoting HO pathogenesis are unclear. In this study, we found that mast cell-specific deletion attenuated localized inflammation, with marked inhibition endochondral osteogenesis. Likewise, blockage nerve...