A5048906778- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Protein Tyrosine Phosphatases
- Animal Behavior and Welfare Studies
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Animal Nutrition and Physiology
- melanin and skin pigmentation
- 3D Printing in Biomedical Research
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Neonatal Respiratory Health Research
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- Galectins and Cancer Biology
- Genetics and Plant Breeding
- Livestock Farming and Management
- Reproductive Biology and Fertility
- Metabolism, Diabetes, and Cancer
Cincinnati Children's Hospital Medical Center
2024
University of Cincinnati
2024
Wen's Food Group (China)
2018-2024
Key Laboratory of Guangdong Province
2022-2024
Poultry Research Institute
2024
South China Agricultural University
2017-2022
Wuxi People's Hospital
2021
Nanjing Medical University
2021
University of Minnesota
2016-2020
Genome Institute of Singapore
2019-2020
Background & AimsThere are few in vitro models for studying the 3-dimensional interactions among different liver cell types during organogenesis or disease development. We aimed to generate hepatic organoids that comprise parenchymal and have structural features of liver, using human pluripotent stem cells.MethodsWe cultured H1 embryonic cells (WA-01, passage 27-40) induced (GM23338) with a series chemically defined serum-free media induce formation posterior foregut cells, which were...
Abstract Background Uncovering the genetic architecture of economic traits in pigs is important for agricultural breeding. However, high-density haplotype reference panels are unavailable most species, limiting accurate genotype imputation large populations. Moreover, infinitesimal model quantitative implies that weak association signals tend to be spread across genome, further complicating analysis. Hence, there a need develop new methods sequencing cohorts without panels. Results We...
Abstract Genetic mapping to identify genes and alleles associated with or causing economically important quantitative trait variation in livestock animals such as pigs is a major goal animal genetic improvement. Despite recent advances high-throughput genotyping technologies, the resolution of remains poor due part low density genotyped variant sites. In this study, we overcame limitation by developing reference haplotype panel for based on 2259 whole genome-sequenced representing 44 pig...
Antibody-based therapies have better specificity and thus improved efficacy over standard chemotherapy regimens, which result in extended survival quality of life for cancer patients. Because antibodies are viewed as too large to access intracellular locations, antibody therapy has traditionally targeted extracellular or secreted proteins expressed by cells. However, many oncogenic found within the cell (such phosphatases/kinases transcription factors) therefore not been pursued therapies....
Combination with other small molecule drugs represents a promising strategy to improve therapeutic efficacy of FLT3 inhibitors in the clinic. We demonstrated that combining ABT-869, inhibitor, SAHA, HDAC led synergistic killing AML cells mutations and suppression colony formation. identified core gene signature is uniquely induced by combination treatment 2 different leukemia cell lines. Among these, we showed downregulation PTP4A3 (PRL-3) played role this synergism. PRL-3 downstream...
The number of teats in pigs is related to a sow's ability rear piglets weaning age. Several studies have identified genes and genomic regions that affect teat swine but few common results were reported. objective this study was identify genetic factors pigs, evaluate the accuracy prediction, contribution significant broad-sense heritability prediction using 41,108 autosomal single nucleotide polymorphisms (SNPs) from genotyping-by-sequencing on 2936 Duroc boars. Narrow-sense dominance...
The availability of a unique unselected Holstein line since 1964 provided direct comparison between selected and genomes whereas large samples unprecedented statistical power for identifying high-confidence SNP effects. Utilizing these resources, we aimed to identify genome changes affected by selection 1964.Direct genome-wide markers contemporary Holsteins showed that the 40 years artificial resulted in landscape changes. Among regions selection, containing 198 genes with fertility...
Summary To investigate the co-development of vasculature, mesenchyme, and epithelium crucial for organogenesis acquisition organ-specific characteristics, we constructed a human pluripotent stem cell-derived organoid system comprising lung or intestinal surrounded by organotypic mesenchyme vasculature. We demonstrated pivotal role co-differentiating mesoderm endoderm via precise BMP regulation in generating multilineage organoids gut tube patterning. Single-cell RNA-seq analysis revealed...
AbstractPRL-1 (phosphatase of regenerating liver-1), PRL-2, and PRL-3 are protein tyrosine phosphatases with a C-terminal prenylation motif that localized to the inner leaflet plasma membrane early endosomes. A variety metastatic PRL-overexpressing cancers have been reported. Therefore, three PRL-phosphatases represent an intriguing group proteins being validated as biomarkers therapeutic targets in cancer. Targeting intracellular PRLs prevent cancer metastasis by exogenous reagents is...
Ke Guo 1,2 , Jing Ping Tang Li Jie Abdul Qader O. Al-Aidaroos 2 Cheng William Hong 3 Peow Bobby Tan Jung Eun Park Leyon Varghese Zhiwei Feng 4 Jianbiao Zhou 5 Wee Joo Chng 6 and Qi Zeng 2,7 1 Denotes equal contribution Institute of Molecular Cell Biology, A*STAR (Agency for Science, Technology Research), 61 Biopolis Drive, Proteos, Singapore 138648, Republic Cleveland Clinic Lerner College Medicine, 9500 Euclid Avenue, Cleveland, OH 44195, USA Nanyang Technological University, School...
Univariate or bivariate animal models were used to estimate the variance components and co-variance for eight reproductive traits: total number born (TNB), alive (NBA), litter weight of piglets (BALWT), healthy births (NHB), weak (NWB), deformed fetuses (NDF), stillborn (NSB), mummified pigs (MUMM). In addition, phenotypic genetic correlations between traits at different parities also estimated. The results showed that heritabilities lower than 0.10. Genetic NHB TNB, NBA, BALWT 0.68, 0.84...
Curcumin, traditionally used as food and medicinal purposes, has recently been reported to have protective efficacy against hypoxia. Hypoxia is one of the important reactive factors in tumor metastasis, which a key problem clinical thyroid cancer therapy. In present study, we investigate anti-metastatic effect curcumin on K1 papillary cells well its potential mechanisms. The results show that effectively inhibits hypoxia-induced oxygen species (ROS) upregulation significantly decreases mRNA...
Inbreeding and relatedness in wild panda populations are important parameters for conservation. Habitat loss fragmentation expected to increase inbreeding but the actual levels natural habitats were unknown. Using 150,025 SNPs 14,926 selected from published whole-genome sequences, we estimated genomic coefficients of 49 pandas including 34 sampled six habitats. Qinling Liangshan had highest measured by coancestry coefficients, whereas Qionglai Minshan 28–45% those Liangshan. Genomic between...
The distinct states of pluripotency in the pre- and post-implantation embryo can be captured vitro as naive primed pluripotent stem cell cultures, respectively. study application state remains hampered, particularly humans, partially due to current culture protocols relying on extraneous undefined factors such feeders. Here we performed a small-molecule screen identify compounds that facilitate chemically defined establishment maintenance human feeder-independent embryonic (FINE) cells....
Abstract Background Genomic selection using single nucleotide polymorphism (SNP) markers has been widely used for genetic improvement of livestock, but most current methods genomic are based on SNP models. In this study, we investigated the prediction accuracies haplotype models fixed chromosome distances and gene boundaries compared to those phenotypic values. We also examined reasons successes failures prediction. Methods analyzed a swine population 3195 Duroc boars with records eight...
Abstract Background VHZ is a VH1-like (member Z) dual specific protein phosphatase encoded by DUSP23 gene. Some of the phosphatases (DSPs) play an important role in cell cycle control and have shown to be associated with carcinogenesis. Here, expression growth human cancers was investigated. Results We generated mouse monoclonal antibody (mAb clone#209) rabbit polyclonal antibodies (rAb) against VHZ. performed proliferation assay learn how retroviral transduction express VHZ, VHZ(C95S),...
Genomic prediction using multi-allelic haplotype models improved the accuracy for all seven human phenotypes, normality transformed high density lipoproteins, low total cholesterol, triglycerides, weight, and original height body mass index without transformation. Eight SNP sets with 40,941-380,705 SNPs were evaluated. The increase in due to haplotypes was 1.86-8.12%. Haplotypes fixed chromosome distances had best four number of two gene-based lipoproteins (tied best). coding genes more...
Body length, body height, and total teat number are economically important traits in pig breeding, as these usually associated with the growth, reproductivity, longevity potential of piglets. Here, we report a genetic analysis using population comprising 2,068 Large White pigs. A genotyping-by-sequencing (GBS) approach was used to provide high-density genome-wide SNP discovery genotyping. Univariate bivariate animal models were estimate heritability correlations. The results showed that...
(1) Background: Reproductive performance is crucial for the pork industry’s success. The Large White pig central to this, yet genetic factors influencing its reproductive traits are not well understood, highlighting need further research. (2) Methods: This study utilized Genome-Wide Association Studies explore basis of in pig. We collected data from 2237 sows across four breeding herds southern China, focusing on eight traits. Statistical analyses included principal component analysis,...
The present study aimed to observe the growth of transplanted tumors in mice with colorectal cancer (CRC) under hyperglycemic conditions and detect expression vascular endothelial factor (VEGF) these tumors. also changes serum insulin-like factor-1 (IGF-1) levels determine whether type 2 diabetes mellitus (T2DM) was a risk for progression development CRC. A mouse model tumor T2DM established volume size tumor. Mice were sacrificed at end 5th week IGF-1 level VEGF tissues. (1628.5 ± 882 mm3)...
Haplotype prediction models open many possibilities to improve the accuracy of genomic selection but require more data processing and computing time than single-SNP models. To facilitate haplotype analysis for estimation using structural functional information, we developed a pipeline implement with capabilities preparation input analysis, GVCHAP, GVCHAP results. Data includes utility programs imputing; defining blocks by fixed number SNPs, distance in base pairs per block, or user defined...