A5012387205- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Protein Degradation and Inhibitors
- Asymmetric Hydrogenation and Catalysis
- Diabetes Treatment and Management
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Inflammatory Bowel Disease
- Biochemical and Molecular Research
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- CAR-T cell therapy research
- Catalytic C–H Functionalization Methods
- Receptor Mechanisms and Signaling
- HIV/AIDS drug development and treatment
- Advanced Sensor and Energy Harvesting Materials
- MicroRNA in disease regulation
- Luminescence and Fluorescent Materials
- Cancer-related molecular mechanisms research
- Surface Modification and Superhydrophobicity
- Asymmetric Synthesis and Catalysis
- Genomics and Phylogenetic Studies
- Catalytic Cross-Coupling Reactions
Chengdu Medical College
2025
Northeastern University
2024
Central South University
2024
Shanghai Tunnel Engineering (China)
2024
Shanghai Tenth People's Hospital
2020-2023
Tongji University
2020-2023
Northwest University
2023
Ningxia University
2021-2023
Shanxi Agricultural University
2018-2023
University of Michigan
2023
The highly enantioselective hydrogenation of quinoline derivatives is developed using [Ir(COD)Cl]2/(R)-MeO-Biphep/I2 system, and this methodology has been applied to the asymmetric synthesis three naturally occurring alkaloids angustureine, galipinine, cuspareine. This method provided an efficient access a variety optically active tetrahydroquinolines with up 96% ee.
A cell-based phenotypic screen for inhibitors of biofilm formation in mycobacteria identified the small molecule TCA1, which has bactericidal activity against both drug-susceptible and -resistant Mycobacterium tuberculosis (Mtb) sterilizes Mtb vitro combined with rifampicin or isoniazid. In addition, TCA1 nonreplicating is efficacious acute chronic infection mouse models alone Transcriptional analysis revealed that down-regulates genes known to be involved persistence. Genetic affinity-based...
Liquid manipulation is essential for daily life and modern industry, it widely used in various fields, including seawater desalination, microfluidic robots, biomedical engineering. Nevertheless, the current research focuses on of individual droplets. There are a few projects water film management. Here, we proposed facile method wind-triggered self-sculpturing based heterogeneous wettability surface, which achieved by femtosecond laser direct writing technology deposition. Under conditions...
Orlistat, or tetrahydrolipstatin (THL), is an FDA-approved antiobesity drug with potential antitumor activities. Cellular off-targets and side effects of Orlistat in cancer therapies, however, have not been extensively explored thus far. In this study, we report the total synthesis THL-like protein-reactive probes, which extremely conservative modifications (i.e., alkyne handle) were introduced parental THL structure to maintain native biological properties while providing necessary...
Mitochondrial dynamics: An image-based screen identified a small molecule, M1, that specifically promotes the fusion of fragmented mitochondria and protects cells from mitochondrial-fragmentation-associated cell death. Mechanistic studies revealed M1 shifts mitochondrial dynamic balance towards (see picture).
Fluorescence imaging provides an indispensable way to locate and monitor biological targets within complex dynamic intracellular environments. Of the various agents currently available, small molecule-based probes provide a powerful tool for live cell imaging, primarily due their desirable properties, including permeability (as result of smaller sizes), chemical tractability (e.g., different molecular structures/designs can be installed), amenability wide variety events. With few exceptions,...
Abstract Chimeric antigen receptor T (CAR‐T) cells have demonstrated promising results against hematological malignancies, but encountered significant challenges in translation to solid tumors. To overcome these hurdles, we developed a switchable CAR‐T cell platform which the activity of engineered is controlled by dosage an antibody‐based switch. Herein, apply this approach Her2‐expressing breast cancers engineering switch molecules through site‐specific incorporation FITC or grafting...
The present study was designed to evaluate the effects of matrine (MAT) on D‐galactose‐ (D‐gal‐) induced aging and relative mechanism. Vitamin E at dose 100 mg/kg used as a standard positive control. MAT significantly improved D‐gal‐induced recognition spatial memory impairment in novel object Y maze tests, exercise endurance decreased weight‐loaded swimming test 2 10 mg/kg. We found that D‐gal treatment noticeably aging‐related changes such reducing thymus coefficients, increasing...
Significance Thiopeptides are a subclass of ribosomally synthesized natural products with complex structures and potent antimicrobial activities. Here we describe general strategy that allows the incorporation noncanonical amino acids into thiopeptides by introducing orthogonal amber suppressor aminoacyl-tRNA synthetase/tRNA pairs thiocillin-producing strain Bacillus cereus . We show thiocillin variants harboring acid bioorthogonal chemical reactivity can be further modified to create probes...
Selected: A tryptophanyl-tRNA synthetase(TrpRS)/tRNA pair was evolved to genetically encode tryptophan analogues and other unnatural amino acids with large side chains in E. coli. selection scheme employed identify TrpRS variants able selectively charge tRNACUATrp Trp (see picture). Substitution of Trp66 enhanced cyan fluorescent protein (ECFP) these afforded ECFP novel spectral properties. As a service our authors readers, this journal provides supporting information supplied by the...
Significance Many therapeutic peptides suffer from short plasma half-lives and, as a consequence, require frequent injections to be therapeutically effective; this in turn can adversely affect patient compliance. Here, we describe the development of novel peptide engineering strategy that incorporates serum protein binding motif into covalent side-chain staple. This approach was used generate stapled long-acting glucagon-like peptide-1 analogs with potency comparable exendin-4 and...
Glucagon-like peptide 2 (GLP-2) is a hormone that has been shown to stimulate intestinal growth and attenuate inflammation. Despite being efficacious in variety of animal models disease, its therapeutic potential hampered by the short half-life vivo. We now describe highly potent, stapled long-acting GLP-2 analog, 10, more than 10-fold longer teduglutide improved intestinotrophic anti-inflammatory effects mouse DSS-induced colitis.
Abstract Background Creeping fat (CrF) has been recognized to play a positive role in Crohn’s disease (CD) progression, yet the cellular compositions within mesenteric adipose tissue (MAT) and their potential mechanism CrF formation are poorly understood. Methods Analysis of 10X single-cell RNA sequencing was performed on 67 064 cells from 3 pairs surgically resected samples uninvolved MAT. The results were validated another cohort with 6 paired MAT by immunofluorescence. Results All...
Advanced process technology allows high memory density while securing bandwidth. However, the increasing disparity between computing unit and known as wall impedes applications like artificial intelligence (AI) related workloads. The larger area of cell introduces more defects, this causes a yield problem. Error-correction code (ECC) is widely used technique in modern computer architecture for system robustness. overhead introduced by ECC limits performance certain timing-critical...
Hemoglobin-based carbon monoxide carrier (HBCOC) can dissociate and ameliorate organ damage by inhibiting inflammation oxidative stress. In this study, we evaluated its effect on cerebral ischemia-reperfusion injury in mice explored potential mechanism. A middle artery occlusion/reperfusion (MCAO/R) mouse model was established using the wire embolization method, HBCOC or equivalent normal saline administered via tail vein during reperfusion. HE staining TEM were used to observe tissue. The...
A ∼3500-member library of bidentate inhibitors against protein tyrosine phosphatases (PTPs) was rapidly assembled using click chemistry. Subsequent high-throughput screening had led to the discovery highly potent (Ki as low 150 nM) and selective MptpB inhibitors, some which represent most developed date.
A key challenge in current drug discovery is the development of high-throughput (HT) amenable chemical reactions that allow rapid synthesis diverse libraries enzyme inhibitors. The Cu(I)-catalyzed, 1,3-dipolar cycloaddition between an azide and alkyne, better known as "click chemistry", one such method has received most attention recent years. Despite its popularity, there still a lack robust efficient strategies give access to azide-containing building blocks (key components click...