A5047822734- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Liver Disease and Transplantation
- Viral Infections and Immunology Research
- Infection Control and Ventilation
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
- HIV/AIDS drug development and treatment
- Synthesis of β-Lactam Compounds
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Pneumocystis jirovecii pneumonia detection and treatment
- Respiratory viral infections research
- Salmonella and Campylobacter epidemiology
- RNA Research and Splicing
Health Canada
2016-2025
University of Ottawa
2012-2025
University of Guelph
2023
University of British Columbia
2023
Public Health Agency of Canada
2023
Government of Canada
2021
National Research Council Canada
2011-2015
Steacie Institute for Molecular Sciences
2010-2012
MicroRNAs (miRNAs) are small RNAs that posttranscriptionally regulate gene expression. Their aberrant expression is commonly linked with diseased states, including hepatitis C virus (HCV) infection. Herein, we demonstrate HCV replication induces the of miR-27 in cell culture and vivo infectious models. Overexpression proteins core NS4B independently activates Furthermore, establish overexpression hepatocytes results larger more abundant lipid droplets, as observed by coherent anti-Stokes...
Automated on-chip sample-to-answer workflow for multiplexed colorimetric detection of foodborne viruses using an embedded polymer micropillar array read-out.
ABSTRACT Human noroviruses are the leading cause of non-bacterial shellfish-associated gastroenteritis. In 2022, a multi-jurisdictional norovirus outbreak associated with contaminated oysters occurred that involved hundreds illnesses. Here, we conducted genetic analysis on 30 clinical samples this oyster outbreak. We first determined capsid genotypes by Sanger sequencing and viral titers droplet-digital reverse transcription PCR. Multiple were identified in outbreak, which could indicate...
Foodborne viruses such as human norovirus (HuNoV) and hepatitis A virus (HAV) are the major causes of foodborne illnesses worldwide. These have a low infectious dose persistent in environment food for weeks. Ready-to-eat (RTE) moisture foods (LMFs) undergo minimal pathogen reduction processes. In recent years, multiple HAV outbreaks involving hundreds individuals were associated with consumption dates, indicating that they could be important vehicles infection. There is no standard method...
The recent spillover events of highly pathogenic avian influenza (HPAI) A(H5N1) clade 2.3.4.4b to dairy cattle, and high viral shedding in the milk from infected animals, has created concern that products could be a route for human infection. It been demonstrated pasteurization is effective inactivating milk. However, multiple are made with unpasteurized but thermised aim this study was examine whether some conditions commonly used thermisation against inactivation whole For purpose, we...
Norovirus (NoV) is the leading cause of gastroenteritis worldwide. A robust cell culture system does not exist for NoV and therefore detailed characterization outbreak sporadic strains relies on molecular techniques. In this study, we employed a metagenomic approach that uses non-specific amplification followed by next-generation sequencing to whole genome sequence genomes directly from clinical samples obtained 8 linked patients. Enough depth was each sample use de novo assembly...
Human coronaviruses, including SARS-CoV-2, are known to spread mainly via close contact and respiratory droplets. However, other potential means of transmission may be present. Fomite-mediated occurs when viruses deposited onto a surface then transfer subsequent individual. Surfaces can become contaminated directly from droplets or hand. Due mask mandates in many countries around the world, former is less likely. Hands if on them (i.e., coughing sneezing) through with fecal material where...
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in oyster’s digestive glands, making RNA extraction RT-PCR detection difficult due to complex nature food matrix presence inhibitors. Herein, we have developed a viral protocol from oysters using murine (MNV) surrogate for human noroviruses. The method combines lysis Tri-Reagent reagent, followed by Direct-Zol...
We tested liver samples from 372 Norway rats (Rattus norvegicus) southern Ontario, Canada, during 2018-2021 to investigate presence of hepatitis E virus infection. Overall, 21 (5.6%) positive for the virus. Sequence analysis demonstrated all infections be rat (Rocahepevirus ratti genotype C1).
Abstract Phosphatidylinositol kinases (PIKs) are key enzymatic regulators of membrane phospholipids and environments that control many aspects cellular function, from signal transduction to secretion, through the Golgi apparatus. Here, we have developed a photoreactive “clickable” probe, PIK‐BPyne, report activity PIKs. We investigated selectivity efficiency probe both inhibit label PIKs, compared PIK‐BPyne wortmannin activity‐based also known target found can act as an effective in situ for...
Human norovirus is the leading cause of viral gastroenteritis globally, and GII.4 has been most predominant genotype for decades. This numerous variants that have caused repeated epidemics worldwide. However, molecular evolutionary signatures among not elucidated throughout genome. A metagenomic, next-generation sequencing method, based on Illumina RNA-Seq, was applied to determine sequences from clinical samples. Herein, obtained deep-sequencing data employed analyze full-genomic prevailing...
Abstract Highly pathogenic avian influenza (HPAI) clade 2.3.4.4b H5Nx viruses continue to cause episodic incursions and have been detected in more than 12 taxonomic orders encompassing 80 species, land marine mammals, including recent detections dairy cattle. The HPAI H5N1 spillover these important livestock species creates a new interface for human exposure raises food safety concerns. Presence of genetic material one out five retail pasteurized milk samples the USA has prompted evaluation...