A5078147441- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Bipolar Disorder and Treatment
- Amino Acid Enzymes and Metabolism
- Renal and related cancers
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Fungal and yeast genetics research
- Telomeres, Telomerase, and Senescence
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
Université Paris Cité
2015-2023
Institut de recherche Saint-Louis
2018-2023
Hôpital Saint-Louis
2019-2023
Institut de Biologie Paris-Seine
2015-2023
Sorbonne Université
2015-2023
Centre National de la Recherche Scientifique
2015-2023
Transgene (France)
2023
Inserm
2019-2022
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2022
Laidlaw College
2022
Abstract Transposable element activity is repressed in the germline animals by PIWI-interacting RNAs (piRNAs), a class of small produced genomic loci mostly composed TE sequences. The mechanism induction piRNA production these still enigmatic. We have shown that, Drosophila melanogaster, cluster tandemly repeated P-lacZ-white transgenes can be activated for maternal inheritance cytoplasm containing homologous piRNAs. This state stably transmitted over generations and allows trans-silencing...
Article17 July 2020Open Access Source Data A small targeting domain in Ty1 integrase is sufficient to direct retrotransposon integration upstream of tRNA genes Amna Asif-Laidin orcid.org/0000-0001-7563-4196 INSERM U944, CNRS UMR 7212, Genomes& Cell Biology Disease Unit, Institut de Recherche Saint-Louis, Hôpital Université Paris, France Search for more papers by this author Christine Conesa CEA, CNRS, Institute Integrative the (I2BC), Paris-Saclay, Gif-sur-Yvette, Amandine Bonnet...
Two Telomeric Associated Sequences, TAS-R and TAS-L, form the principal subtelomeric repeat families identified in Drosophila melanogaster. They are PIWI-interacting RNA (piRNA) clusters involved repression of Transposable Elements. In this study, we revisited TAS structural functional dynamics D. melanogaster related species. silico analysis revealed that family members composed previously uncharacterized domains. This also showed TAS-L repeats arrays a region have named "TAS-L like" (TLL)...
A fraction of all genomes is composed transposable elements (TEs) whose mobility needs to be carefully controlled. In gonads, TE activity repressed by PIWI-interacting RNAs (piRNAs), a class small synthesized heterochromatic loci enriched in fragments, called piRNA clusters. Maintenance active clusters across generations secured maternal inheritance providing the memory for repression. On rare occasions, encounter horizontal transfer (HT) new TEs with no targeting them, threatening host...
Abstract The yeast Ty1 retrotransposon integrates upstream of genes transcribed by RNA polymerase III (Pol III). Specificity integration is mediated an interaction between the integrase (IN1) and Pol III, currently uncharacterized at atomic level. We report cryo-EM structures in complex with IN1, revealing a 16-residue segment IN1 C-terminus that contacts subunits AC40 AC19, we validate vivo mutational analysis. Binding to associates allosteric changes may affect its transcriptional...
SUMMARY Integration of transposable elements into the genome is mutagenic. Mechanisms that target integration relatively safe locations and minimize deleterious consequences for cell fitness have emerged during evolution. In budding yeast, Ty1 LTR retrotransposon upstream RNA polymerase III (Pol III)-transcribed genes requires interaction between AC40 subunit Pol integrase (IN1). Here we show IN1-AC40 involves a short linker sequence in bipartite nuclear localization signal (bNLS) IN1....
Summary The yeast Ty1 retrotransposon integrates upstream of genes transcribed by RNA polymerase III (Pol III). Specificity integration is mediated an interaction between the integrase (IN1) and Pol III, currently uncharacterized at atomic level. Here, we report cryo-EM structures in complex with IN1, revealing a 16-residue segment IN1 C-terminus that contacts subunits AC40 AC19, validate vivo mutational analysis. Unexpectedly, binding associates insertion subunit C11 C-terminal Zn-ribbon...
Abstract Background Transposable elements are ubiquitous and play a fundamental role in shaping genomes during evolution. Since excessive transposition can be mutagenic, mechanisms exist the cells to keep these mobile under control. Although many cellular factors regulating mobility of retrovirus-like transposon Ty1 Saccharomyces cerevisiae have been identified genetic screens, only very few them interact physically with integrase (IN). Results Here, we perform proteomic screen establish IN...
Abstract Background. Transposable elements are ubiquitous and play a fundamental role in shaping genomes during evolution. Since excessive transposition can be mutagenic, mechanisms exist the cells to keep these mobile under control. Although many cellular factors regulating mobility of retrovirus-like transposon Ty1 Saccharomyces cerevisiae have been identified genetic screens, only very few them interact physically with integrase (IN). Results. Here, we perform proteomic screen establish...
Transposable elements are ubiquitous and play a fundamental role in shaping genomes during evolution. Since excessive transposition can be mutagenic, mechanisms exist the cells to keep these mobile under control. Although many cellular factors regulating mobility of retrovirus-like transposon Ty1 Saccharomyces cerevisiae have been identified genetic screens, only very few them interact physically with integrase (IN).Here, we perform proteomic screen establish IN interactome. Among 265...