A5065810212- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Parasite Biology and Host Interactions
- Marine Biology and Ecology Research
- Nematode management and characterization studies
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Animal Genetics and Reproduction
- Fungal and yeast genetics research
- RNA Research and Splicing
- HVDC Systems and Fault Protection
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Genetic diversity and population structure
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Genetic and Mutation Studies
- Telomeres, Telomerase, and Senescence
Institut de Biologie Paris-Seine
2015-2024
Transgene (France)
2019-2023
Laboratoire de Biologie du Développement
2008-2023
Sorbonne Université
2010-2023
Centre National de la Recherche Scientifique
2007-2023
Centre National pour la Recherche Scientifique et Technique (CNRST)
2021
Université Paris Cité
2005-2015
Epigénétique et Destin Cellulaire
2015
Bipar
2013
Institut Jacques Monod
2005-2012
The study of P-element repression in Drosophila melanogaster led to the discovery telomeric Trans-Silencing Effect (TSE), a mechanism by which transposon or transgene inserted subtelomeric heterochromatin (Telomeric Associated Sequence TAS) has capacity repress trans female germline, homologous transposon, located euchromatin. TSE shows variegation among egg chambers ovaries when silencing is incomplete. Here, we report that displays an epigenetic transmission through meiosis, involves...
Abstract Transposable element activity is repressed in the germline animals by PIWI-interacting RNAs (piRNAs), a class of small produced genomic loci mostly composed TE sequences. The mechanism induction piRNA production these still enigmatic. We have shown that, Drosophila melanogaster, cluster tandemly repeated P-lacZ-white transgenes can be activated for maternal inheritance cytoplasm containing homologous piRNAs. This state stably transmitted over generations and allows trans-silencing...
Abstract 2′-O-Methylation (Nm) represents one of the most common RNA modifications. Nm affects structure and function with crucial roles in various RNA-mediated processes ranging from silencing, translation, self versus non-self recognition to viral defense mechanisms. Here, we identify two methyltransferases (Nm-MTases) Drosophila melanogaster (CG7009 CG5220) as functional orthologs yeast TRM7 human FTSJ1. Genetic knockout studies together MALDI-TOF mass spectrometry RiboMethSeq mapping...
The study of P transposable element repression in Drosophila melanogaster led to the discovery Trans-Silencing Effect (TSE), a homology-dependent mechanism by which P-transgene inserted subtelomeric heterochromatin (Telomeric Associated Sequences, "TAS") has capacity repress trans, female germline, homologous P-lacZ transgene located euchromatin. Phenotypic and genetic analysis have shown that TSE exhibits variegation ovaries, displays maternal effect as well epigenetic transmission through...
Transposable element (TE) activity is repressed in animal gonads by PIWI-interacting RNAs (piRNAs) produced piRNA clusters. Current models flies propose that germinal clusters are functionally defined the maternal inheritance of piRNAs during previous generation. Taking advantage an inactive, but ready to go, cluster P-element derived transgene insertions Drosophila melanogaster, we show here raising at high temperature (29°C) instead 25°C triggers stable conversion this locus from inactive...
Two Telomeric Associated Sequences, TAS-R and TAS-L, form the principal subtelomeric repeat families identified in Drosophila melanogaster. They are PIWI-interacting RNA (piRNA) clusters involved repression of Transposable Elements. In this study, we revisited TAS structural functional dynamics D. melanogaster related species. silico analysis revealed that family members composed previously uncharacterized domains. This also showed TAS-L repeats arrays a region have named "TAS-L like" (TLL)...
The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since no coding capacity, its mobilization requires transposase-encoding element. On basis similarity TIRs target-site preference with autonomous Tc1-like impala, together correlated distribution both among genus, we investigated...
ABSTRACT The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that ENV-like ORF3 mediates viral infectivity. We have produced 293GP/LNhsp70lucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by protein. shown immunostaining envelope protein cells and particles collected from these can infect cells. Our results provide direct evidence infectious property due to its gene product.
FTSJ1 is a conserved human 2'-O-methyltransferase (Nm-MTase) that modifies several tRNAs at position 32 and the wobble 34 in anticodon loop. Its loss of function has been linked to X-linked intellectual disability (XLID), more recently cancers. However, molecular mechanisms underlying these pathologies are currently unclear. Here, we report novel pathogenic variant from an patient. Using blood cells derived this patient other affected individuals carrying mutations, performed unbiased...
A fraction of all genomes is composed transposable elements (TEs) whose mobility needs to be carefully controlled. In gonads, TE activity repressed by PIWI-interacting RNAs (piRNAs), a class small synthesized heterochromatic loci enriched in fragments, called piRNA clusters. Maintenance active clusters across generations secured maternal inheritance providing the memory for repression. On rare occasions, encounter horizontal transfer (HT) new TEs with no targeting them, threatening host...
Background The study of P element repression in Drosophila melanogaster led to the discovery telomeric Trans-Silencing Effect (TSE), a homology-dependent mechanism by which P-transgene inserted subtelomeric heterochromatin (Telomeric Associated Sequences, "TAS") has capacity repress trans, female germline, homologous P-lacZ transgene located euchromatin. TSE can show variegation ovaries, displays maternal effect as well an epigenetic transmission through meiosis and involves RNA silencing...
The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. Although these proteins are known to be necessary for protein processing, transcription, integration, respectively, the function thought Gag has not been determined. We present here first electron microscopy particles. tested whether putative was required particle formation, packaging RNA, transcription. generated...
Abstract The study of P transposable element repression in Drosophila melanogaster led to the discovery trans-silencing effect (TSE), a homology-dependent mechanism by which P-transgene inserted subtelomeric heterochromatin (Telomeric Associated Sequences) represses trans, female germline, homologous P-lacZ transgene euchromatin. TSE shows variegation ovaries and displays maternal as well epigenetic transmission through meiosis. In addition, is highly sensitive mutations affecting components...
Transposable element (TE) activity is repressed in the Drosophila germline by Piwi-Interacting RNAs (piRNAs), a class of small non-coding RNAs. These piRNAs are produced discrete genomic loci containing TE fragments. In recent publication, we tested for existence strict epigenetic induction piRNA production capacity locus D. melanogaster genome. We used 2 lines carrying transgenic 7-copy tandem cluster (P-lacZ-white) at same site. This generates both local heterochromatic sector. One line...
Genome integrity of the animal germline is protected from transposable element activity by PIWI-interacting RNAs (piRNAs). While piRNA biogenesis intensively explored, little known about genetical determination clusters, genomic sources piRNAs. Using a bimodal epigenetic state cluster (BX2), we identified histone demethylase Kdm3 as being able to prevent cryptic production. In absence Kdm3, dozens coding gene-containing regions become genuine dual-strand clusters. Eggs laid mutant females...
With the expansion of high throughput sequencing, we now have access to a larger number genome-wide studies analyzing Transposable elements (TEs) composition in wide variety organisms. However, genomic analyses often remain too limited and diversity species investigated study depth dynamics evolutionary success different types TEs among metazoans. Therefore, chose investigate use transcriptomes describe phylogenetically related by conducting first comparative analysis two groups polychaetes...
The study of P-element repression in Drosophila melanogaster led to the discovery telomeric Trans-Silencing Effect (TSE), a mechanism by which transposon or transgene inserted subtelomeric heterochromatin (Telomeric Associated Sequence TAS) has capacity repress trans female germline, homologous transposon, located euchromatin.TSE shows variegation among egg chambers ovaries when silencing is incomplete.Here, we report that TSE displays an epigenetic transmission through meiosis, involves...