A5036582269- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- HVDC Systems and Fault Protection
- Education, Technology, and Economics
- Genomics and Phylogenetic Studies
- Library Science and Information Literacy
- Banking Systems and Strategies
- RNA Research and Splicing
- Parasitic Infections and Diagnostics
Laboratoire de Biologie du Développement
2018-2023
Columbia University
2022
Transgene (France)
2019-2021
Sorbonne Université
2019-2021
Institut de Biologie Paris-Seine
2018-2021
Centre National de la Recherche Scientifique
2019-2020
Abstract 2′-O-Methylation (Nm) represents one of the most common RNA modifications. Nm affects structure and function with crucial roles in various RNA-mediated processes ranging from silencing, translation, self versus non-self recognition to viral defense mechanisms. Here, we identify two methyltransferases (Nm-MTases) Drosophila melanogaster (CG7009 CG5220) as functional orthologs yeast TRM7 human FTSJ1. Genetic knockout studies together MALDI-TOF mass spectrometry RiboMethSeq mapping...
tRNA fragments (tRFs) are a class of small non-coding RNAs (sncRNAs) derived from tRNAs. tRFs highly abundant in many cell types including stem cells and cancer cells, found all domains life. Beyond translation control, have several functions ranging transposon silencing to proliferation control. However, the analysis presents specific challenges their biogenesis is not well understood. They very heterogeneous modified by numerous post-transcriptional modifications. Here we describe...
FTSJ1 is a conserved human 2'-O-methyltransferase (Nm-MTase) that modifies several tRNAs at position 32 and the wobble 34 in anticodon loop. Its loss of function has been linked to X-linked intellectual disability (XLID), more recently cancers. However, molecular mechanisms underlying these pathologies are currently unclear. Here, we report novel pathogenic variant from an patient. Using blood cells derived this patient other affected individuals carrying mutations, performed unbiased...
Abstract DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of prokaryotes: N 6 -methyladenine (6mA), 4 -methylcytosine (4mC), 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types methylation, few technologies can be used for efficiently mapping multiple methylation. Here, we present NT-seq all simultaneously. reliably detects known motifs two bacterial genomes identifying de novo motifs. provides a...
Abstract 2’-O-methylation (Nm) represents one of the most common RNA modifications. Nm affects structure and function with crucial roles in various RNA-mediated processes ranging from silencing, translation, self versus non-self recognition to viral defense mechanisms. Here, we identify two novel methyltransferases (Nm-MTases) Drosophila melanogaster (CG7009 CG5220) as functional orthologs yeast TRM7 human FTSJ1, respectively. Genetic knockout studies together MALDI-TOF mass spectrometry...
Abstract tRNA fragments (tRFs) are a class of small non-coding RNAs (sncRNAs) derived from tRNAs. tRFs highly abundant in many cell types including stem cells and cancer cells, found all domains life. Beyond translation control, have several functions ranging transposon silencing to proliferation control. However, the analysis presents specific challenges their biogenesis is not well understood. They very heterogeneous modified by numerous post-transcriptional modifications. Here we describe...
ABSTRACT FTSJ1 is a conserved human 2’-O-methyltransferase (Nm-MTase) that modifies several transfer RNAs (tRNAs) at position 32 and the wobble 34 in AntiCodon Loop (ACL). Its loss of function has been linked to Non-Syndromic X-Linked Intellectual Disability (NSXLID), more recently cancers. However, molecular mechanisms underlying these pathologies are currently unclear. Here we report novel pathogenic variant from NSXLID patient. Using blood cells derived this patient other affected...