A5007548763- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Testicular diseases and treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Pluripotent Stem Cells Research
- Renal and related cancers
- Xenotransplantation and immune response
- Tissue Engineering and Regenerative Medicine
- LGBTQ Health, Identity, and Policy
- Microbial Metabolic Engineering and Bioproduction
- Urological Disorders and Treatments
- Fungal and yeast genetics research
- Biomedical Research and Pathophysiology
- Ethics and Legal Issues in Pediatric Healthcare
- Epigenetics and DNA Methylation
- Fermentation and Sensory Analysis
- Neonatal Health and Biochemistry
- Animal Genetics and Reproduction
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Childhood Cancer Survivors' Quality of Life
- RNA Interference and Gene Delivery
- Biomedical Ethics and Regulation
Vrije Universiteit Brussel
2016-2025
Ghent University Hospital
2024
University College Ghent
2024
3E (Belgium)
2024
Universitair Ziekenhuis Brussel
2022
Bio-Medical Science (South Korea)
2017-2021
Community Research and Development Information Service
2017
Veterinary and Agrochemical Research Centre
2012
Centre For Human Genetics
2006
Centre d'Enseignement et de Recherches des Industries Alimentaires et Chimiques
1987-1995
Infertility is an important side effect of treatments used for cancer and other non-malignant conditions in males. This may be due to the loss spermatogonial stem cells (SSCs) and/or altered functionality testicular somatic (e.g. Sertoli cells, Leydig cells). Whereas sperm cryopreservation first-line procedure preserve fertility post-pubertal males, this option does not exist prepubertal boys. For patients unable produce at high risk losing their fertility, tissue freezing now proposed as...
Abstract STUDY QUESTION Twenty years after the inception of first fertility preservation programme for pre-pubertal boys, what are current international practices with regard to cryopreservation immature testicular tissue? SUMMARY ANSWER Worldwide, tissue has been cryopreserved from over 3000 boys under age 18 a variety malignant and non-malignant indications; there is variability in related eligibility, clinical assessment, storage, funding. WHAT IS KNOWN ALREADY For male patients receiving...
BACKGROUND: Before clinical application, the feasibility and safety of autologous testicular stem cell transplantation should be explored. Apart from limitations in their numbers, spermatogonial cells may also contaminated by malignant cells. Therefore, both enrichment decontamination before necessary. This study aimed at evaluating decontaminating potential magnetic-activated sorting (MACS) and/or fluorescence-activated (FACS) for murine human suspensions. In mouse, effectiveness technique...
So far, successful de novo formation of testicular tissue followed by complete spermatogenesis in vitro has been achieved only rodents. Our findings reveal that primary human cells are able to self-organize into organoids (TOs), i.e., multi-cellular surrogates, either with or without support a biological scaffold. Despite lacking testis-specific topography, these mini-tissues harbored spermatogonia and their important niche cells, which retained specific functionalities during long-term...
Is there a better alternative to the conventional cryopreservation protocols for human testicular tissue banking?Uncontrolled slow freezing (USF) using 1.5 M dimethylsulphoxide (DMSO) and 0.15 sucrose as cryoprotectants appears be user-friendly efficient method of tissue.Currently, time-consuming controlled (CSF) that need expensive equipment are commonly used banking. USF vitrification techniques were successfully applied in several animal models but further exploration with...
Infertility is a global healthcare problem, and despite long years of assisted reproductive activities, significant number cases remain idiopathic. Our currently restricted understanding basic mechanisms driving human gametogenesis severely limits the improvement clinical care for infertile patients. Using exome sequencing, we identified nonsense mutation leading to premature stop in TEX15 locus (c.2130T>G, p.Y710*) consanguineous Turkish family comprising eight siblings which three brothers...
Abstract STUDY QUESTION Is it possible to co-culture and functionally link human liver testis equivalents in the combined medium circuit of a multi-organ chip? SUMMARY ANSWER Multi-organ-chip co-cultures were maintained at steady-state for least 1 week reproduced specific natural drug-induced liver–testis systemic interactions. WHAT IS KNOWN ALREADY Current benchtop reprotoxicity models typically do not include hepatic metabolism interactions axis. However, these are important study...
BACKGROUND: The xenografting of pre-pubertal human testicular tissue to an immunodeficient mouse is a theoretical strategy for restoring fertility in childhood cancer patients, while circumventing the risk malignant recurrence. This study aimed at comparing grafting and adult murine tissue, as well that two recipients, i.e. Swiss Nude mice SCID-NOD mice. MATERIALS AND METHODS: In this study, we evaluated survival tissues, after subcutaneous RESULTS: After allografting pieces, meiotic cells...
Is it possible to derive a scaffold from human testis for the purpose of tissue engineering and regenerative medicine? We developed method produce cytocompatible decellularized testicular matrix (DTM) while maintaining native tissue-specific characteristics components. The potential benefits scaffolds consisting naturally-derived extracellular (ECM) have been demonstrated using wide variety animal sources. However, so far, never considered constructive remodelling purposes. Human cadaveric...
Grafting of frozen-thawed testicular tissue has been suggested as a novel fertility preservation method for patients undergoing gonadotoxic treatments. However, this technique still needs further optimization before any clinical application. So far, grafting human only performed to the back skin nude mice and shown spermatogonial stem-cell survival occasionally differentiation up primary spermatocytes. In study, orthotopic mouse testes was evaluated an alternative, effect freezing donor's...
When does germ cell loss and fibrosis occur in patients with Klinefelter syndrome (KS)? In KS, is not observed testicular tissue from fetuses the second semester of pregnancy but present at a prepubertal age when architecture still normal, while highly an adolescent age. Most KS are azoospermic adult because massive loss. However, timing this starts known. It assumed that increases puberty. Therefore, sperm extraction (TESE) has been suggested to increase chances retrieval onset...
In vitro spermatogenesis (IVS) has already been successfully achieved in rodents by organotypic and soft matrix culture systems. However, the former does not allow single cell input, latter presents as a simple thick layer which all cells are embedded. We explored new system using mouse model employing an alginate-based hydrogel 3D bioprinting, to control scaffold design deposition. produced testicular constructs consisting of printed cell-free scaffolds (CFS) with prepubertal (TC) their...
Although early development of testes appears normal in boys with Klinefelter syndrome (KS), spermatogonial stem cell (SSC) depletion occurs mid puberty, leading to infertility. Cryopreservation SSCs prior loss is an option that currently offered who have undergo gonadotoxic treatments. This study aimed explore the possibility preserving pubertal KS adolescents by testicular tissue banking.A retrospective was conducted seven non-mosaic 47,XXY adolescents, aged 13-16 years, were invited for...
Can full spermatogenesis be achieved after xenotransplantation of prepubertal primate testis tissue to the mouse, in or subcutaneously?Intratesticular supported differentiation immature germ cells from marmoset (Callithrix jacchus) into spermatids and spermatozoa at 4 9 months post-transplantation, while subcutaneous transplants, spermatogenic arrest was observed none transplants survived months.Auto-transplantation cryopreserved (ITT) could a potential fertility restoration strategy for...