A5008441513- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Trace Elements in Health
- Head and Neck Cancer Studies
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Food Allergy and Anaphylaxis Research
- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Reproductive tract infections research
- Yersinia bacterium, plague, ectoparasites research
- Mass Spectrometry Techniques and Applications
- Cancer Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Nonmelanoma Skin Cancer Studies
- Molecular Junctions and Nanostructures
- Circular RNAs in diseases
- Prostate Cancer Treatment and Research
- Radiopharmaceutical Chemistry and Applications
- Molecular Biology Techniques and Applications
- Cellular Mechanics and Interactions
- Synthesis of Indole Derivatives
Universitat Rovira i Virgili
2014-2024
University of Pardubice
2023
Technical museum in Brno
2023
Masaryk University
2017-2018
Charles University
2014-2018
Central European Institute of Technology
2016-2017
Brno University of Technology
2016-2017
General University Hospital in Prague
2014
Abstract Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-of-need are critical emerging field of personalised medicine which companion diagnostics essential, as well application in low resource settings. Here we report on development a point-of-care lateral flow test direct detection isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to tailed primers, resulting an amplicon with duplex flanked by two...
In this work, different methodologies were evaluated in search of robust, simple, rapid, ultrasensitive, and user-friendly lateral flow aptamer assays. one approach, we developed a competitive based assay, which β-conglutin immobilized on the test line nitrocellulose membrane sample compete for binding to AuNP labeled aptamer. The control exploits an DNA probe complementary aptamer, forcing displacement from β-conglutin-aptamer complex. second competition takes place off-strip, following...
The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the end of 2019, resulting in ongoing COVID-19 pandemic. high transmissibility virus and substantial number asymptomatic individuals have led to an exponential rise infections worldwide, urgently requiring global containment strategies. Reverse transcription-polymerase chain reaction is gold standard for detection SARS-CoV-2 infections. Antigen tests, targeting spike (S) or nucleocapsid (N) viral proteins, are...
The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated species like pufferfish expansion nonendemic areas has increased need for fast and reliable detection effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered most accurate method, although competitive immunoassays have also been reported. In this work, we sought...
Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases length, containing randomised sequence 49 bases. ß-conglutin was purified lupin flour and chemically crosslinked carboxylated magnetic beads. Peptide mass fingerprinting used confirm presence ß-conglutin. Single stranded generated using T7 Gene 6 Exonuclease subsequently incubated with beads captured released amplified prior further round Systematic...
Aptamers are well-established biorecognition molecules used in a wide variety of applications for the detection their respective targets. However, individual SELEX processes typically performed identification aptamers each target can be quite time-consuming, labor-intensive, and costly. An alternative strategy is proposed herein simultaneous different binding distinct but structurally similar targets one single selection. This one-pot approach, using steroids estradiol, progesterone,...
Herein, we describe the in vivo effects of doxorubicin (DOX) encapsulated ubiquitous protein apoferritin (APO) and its efficiency safety anti-tumor treatment. APODOX is both passively (through Enhanced Permeability Retention effect) actively targeted to tumors through prostate-specific membrane antigen (PSMA) via mouse antibodies conjugated surface horse spleen APO. To achieve site-directed conjugation antibodies, a HWRGWVC heptapeptide linker was used. The prostate cancer-targeted...
Trichomoniasis, caused by Trichomonas vaginalis, is the leading nonviral sexually transmitted infection worldwide. We report selection of a DNA aptamer against T. vaginalis adhesion protein, AP65, using microtiter plate-based in vitro combinatorial chemistry process termed systematic evolution ligands exponential enrichment. The enriched library pool was sequenced next-generation sequencing, and several candidates with high affinity specificity were identified. highest had KD low nanomolar...
The overall objective of this work is the evaluation different competitive aptamer assays based on inductively coupled plasma mass spectrometry (ICP-MS) detection for determination β-conglutin (food protein allergen from lupin) in flour samples. To end, two assay schemes were developed using either thiolated aptamers chemisorbed onto gold nanoparticles (AuNPs) or biotinylated linked to streptavidin-AuNPs. influence ICP-MS mode (i.e., conventional vs single particle) performance was explored....
Redox-labeled nucleotides are of increasing interest for the fabrication next generation molecular tools and should meet requirements being thermally stable, sensitive, compatible with polymerase-mediated incorporation while also electrochemically discriminable. The synthesis characterization Keggin Dawson polyoxometalate-deoxynucleotide (POM-dNTP) bioconjugates linked through 7-deaza-modified purines is described. modified POM-dNTPs were used polymerase-based amplification a DNA sequence...
The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility competitive sports but also public health due to well-documented adverse effects they elicit. AAS abuse is restricted professional sports, extends recreational athletes and adolescents well in livestock production growth-promoting agents. Testosterone nandrolone are among most frequently exploited. Gas chromatography-mass spectrometry reference...