A5081544687- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Genital Health and Disease
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Minimally Invasive Surgical Techniques
- Surgical Simulation and Training
- Respiratory viral infections research
- Molecular Biology Techniques and Applications
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Systemic Lupus Erythematosus Research
- COVID-19 diagnosis using AI
- Cancer-related molecular mechanisms research
- Soft Robotics and Applications
- Immunotherapy and Immune Responses
- Virology and Viral Diseases
- Colorectal Cancer Screening and Detection
Chinese University of Hong Kong
2005-2021
Cyberport
2018-2020
Prince of Wales Hospital
2002-2012
National Health Research Institutes
2010
Czech Academy of Sciences, Institute of Microbiology
2002-2005
Shatin Hospital
2004
The recent outbreak of severe acute respiratory syndrome (SARS) provided an opportunity to study the antibody response infected individuals causative virus, SARS coronavirus. We examined serum samples obtained from 46 patients with SARS, 40 non-SARS pneumonia, and 38 healthy individuals, by use Western blotting (WB), enzyme-linked immunoassay (ELISA), immunofluorescence assay, using both native bacterially produced antigens virus. found a highly restricted, immunoglobulin G-dominated in...
Severe acute respiratory syndrome (SARS) is a new human infectious disease with significant morbidity and mortality. The has been shown to be associated coronavirus (SARS-CoV). clinical epidemiological aspects of SARS have described. Moreover, the viral genome SARS-CoV fully sequenced. However, much biological behaviour virus not known data on tissue cellular tropism are limited. In this study, six fatal cases were investigated for using an in-situ hybridization (ISH) technique. Among all...
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected HMPV; 6 these also coronavirus, another 5 (10.4%) coronavirus alone. Using this approach, we found that laryngeal carcinoma (HEp-2) cells superior rhesus monkey kidney (LLC-MK2) commonly previous studies for HMPV. These widely available HEp-2 should be...
Background: Self-management after a stroke is challenge because of multifaceted care needs and complex disabling consequences that cause further hindrance to patient participation. A 13-week empowerment intervention (Health Empowerment Intervention for Stroke [HEISS]) was developed enhance patients’ ability participate in self-management. Purpose: To examine the effects on self-efficacy, self-management behavior, functional recovery. Methods: This single-blind randomized controlled trial...
Human papillomavirus (HPV) type 58 has been found to be prevalent among Chinese patients with cervical cancer. This study examined the oncogenic risk of HPV58 variants in Hong Kong, a southern part China. Altogether, 1924 women were studied: 42.8% normal cervix, 16.2% intraepithelial neoplasia (CIN) I, 12.7% CIN II, 20.8% III, and 7.6% invasive cancer (ICC). The overall prevalence was 11.4% (220) increased statistically significantly severity (Ptrend<.001, χ2 test for trend). Among...
Abstract Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor for HPV52, which the prevalence among squamous cell was underestimated from 14.6% to 0%. The fact that HPV52 should rank second carcinoma Hong Kong could be missed if GP5+/6+, worldwide commonly used set, selected detection. Biases type‐specific...
Abstract Severe acute respiratory syndrome coronavirus (SARS‐CoV) can produce gastrointestinal symptoms. The intestinal tract is the only extrapulmonary site where viable viruses have been detected. This study examined seven established human cell lines, DLD‐1, HCT‐116, HT‐29, LoVo, LS‐180, SW‐480 and SW‐620, for their permissiveness to SARS‐CoV infection. results showed that LoVo cells were permissive infection as evident by positive findings from indirect immunofluorescence staining...
Background. A need for quadrivalent vaccines to cover both lineages of influenza B has been raised. Information on the circulation status and associated hospitalization rates is important assist evidence-based decision making. This retrospective study revealed situation in a subtropical city over 10-year period.
The prevalence of human papillomavirus (HPV) among 332 Hong Kong Chinese women with abnormal Papanicolaou smears were determined by polymerase chain reaction and restriction fragment length polymorphism analysis. overall HPV positive rate was 44.3% 18.6% (16/86) for normal/inflamed cervices, 36.4% (32/88) condyloma, 64.7% (33/51) cervical intraepithelial neoplasia grade 1 (CIN 1), 37.9% (11/29) CIN 2, 68.3 (41/60) 3, 77.8% (14/18) carcinoma. Double infection detected in 17 the 147 samples, a...
Human papillomavirus type 58 (HPV-58) accounts for a much higher proportion of cervical cancers in East Asia than other types. A classification system HPV-58, which is essential molecular epidemiological study, lacking.This study analyzed the sequences 401 isolates collected from 15 countries and cities. The 268 unique concatenated E6-E7-E2-E5-L1-LCR that comprised 57% whole HPV-58 genome showed 4 distinct clusters. L1 LCR produced tree topologies best resembled thus are most appropriate...
This cross-sectional survey assessed the determinants of human papillomavirus (HPV) infections among 2080 women who participated in cervical cancer screening. HPVs were typed by restriction and sequencing analyses. The prevalence HPV was 7.3% (4.2% for high-risk, 1.9% low-risk, 2.1% unknown-risk types). High-risk decreased with age, whereas low- had a second peak older women. Young age only common variable associated 3 groups infections. Lifetime number sex partners high- low-risk types but...
The clinical utility of human papillomavirus (HPV) load and integration status remains unclear. We applied refined methods to delineate the viral load, status, lineage 104 women with HPV‐16 monotype infection, including 19 normal cervices, 9 histologically proven cervical intraepithelial neoplasia (CIN) 1, 24 CIN 2, 27 3, 25 squamous cell carcinoma (SCC). Higher crude as determined by real‐time polymerase chain reaction (PCR) targeting E7 gene, was observed for SCC but became insignificant...
Abstract Controversies exist on the effect of pregnancy human papillomavirus (HPV) infection. A cross‐sectional section study was conducted to compare prevalence and genotype distribution cervical HPV infection between pregnant non‐pregnant women in Hong Kong. Cervical samples were collected from 308 same number age‐matched controls recruited a cancer screening center located at hospital. detected by polymerase chain reaction, followed identification restriction fragment length polymorphism...
A case-control study was conducted on 1986 Hong Kong women to assess the risk of human papillomavirus (HPV) type 16 variants for cervical neoplasia. In total, 255 were HPV-16 positive and analyzed E6 E7 sequence variation. Two novel substitutions at (T86I Q116E) 1 (R66W) found. Most Asian (50.6%) or European (44.3%) lineage, both lineages showed similar associations high-grade invasive No increased observed subclasses variant 350G, which carry a higher cancer in some Western populations. The...
The seroprevalence of antibody to severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in cohorts health care workers (HCWs) with subclinical infection SARS and non-SARS medical wards was 2.3% (3 131 HCWs) 0% (0 192 HCWs), respectively. Rates for clinical SARS-CoV among 742 HCWs on these were highest nurses (11.6%) assistants (11.8%), indicating that occupations are associated the risks exposure.
We undertook a prospective cohort study to ascertain the risk factors for development of squamous intraepithelial lesions (SIL) in patients with systemic lupus erythematosus (SLE).One hundred thirty-seven SLE normal Papanicolaou (Pap) smear at baseline were evaluated 6-month intervals up 3 years. At each visit, Pap smear, human papillomavirus (HPV) DNA test, and clinical assessment performed.Among 137 patients, there 12 incident cases (8.8%) SIL over median followup duration 30.7 months...
This study describes the distribution of hepatitis C genotypes among 106 intravenous drug users and 949 non-drug in Hong Kong. Genotypes were identified by multiplex RT-PCR targeting core region viral genome. The accuracy this typing system identifying 1b 6a was assessed phylogenetic analysis. virus (HCV) amongst 63.6% for genotype 1b, 23.6% 6a, 4.5% 1a, 3.9% 3a, 3.1% 2a; whereas users, it 58.5% 33.0% 5.7% 0.9% 2a. proportion significantly higher (P < 0.001), that lower = 0.001) user group....
Abstract Introduction Prevalence of an abnormal Papanicolaou smear was significantly increased in lupus patients cross-sectional studies, associated with a higher prevalence high-risk human papillomavirus (HPV) infection. The nucleic acid-specific Toll-like receptors (TLRs) locate at the endolysosomal compartments and trigger induction cytokines for innate immune response. This study evaluated whether host response may enhance HPV persistence. Methods Protein levels TLRs 3, 7, 8 9 cervical...
The three novel human herpesviruses (HHV) 6, 7, and 8 are predominantly, but not exclusively, lymphotropic. In an attempt to elucidate their neurotropism in vivo, viral DNA sequences present fresh autopsy cortical brain tissues obtained from 84 consecutive Chinese subjects (mean age, 66.9 years; range, 21–98 years) were detected by a nested polymerase chain reaction. These patients apparently immunocompetent free of clinical signs diseases. HHV-6 was 36 (42.9%) patients, the DNA-positive...
Viral safety is of paramount importance for human plasma-derived therapeutic proteins. Recent reports blood-associated transmission and continuous regional outbreaks dengue fever have prompted a validation clearance virus in the manufacture processes products.A high titer cultured serotype 2 was spiked into process samples before individual steps albumin immunoglobulin processes, including cold ethanol precipitation, cation-exchange chromatography, pasteurization, solvent/detergent...