A5060598281- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Cellular Mechanics and Interactions
- Blood properties and coagulation
- Photoacoustic and Ultrasonic Imaging
- Tendon Structure and Treatment
- Nanoplatforms for cancer theranostics
- Single-cell and spatial transcriptomics
- Inflammatory Biomarkers in Disease Prognosis
Sheffield Hallam University
2021-2023
Single particle characterization has become increasingly relevant for research into extracellular vesicles, progressing from bulk analysis techniques and first-generation to comprehensive multi-parameter measurements such as nano-flow cytometry (nFCM). nFCM is a form of flow that utilizes instrumentation specifically designed nano-particle analysis, allowing thousands EVs be characterized per minute both with without the use staining techniques. High resolution side scatter (SS) detection...
Abstract Extracellular vesicles (EVs) are mediators of intercellular communication, recently recognised for their clinical applications. Accurate characterisation and quantification EVs critical understanding function relevance. Many platforms utilise fluorescence EV characterisation, frequently labelling surface proteins to identify EVs. The heterogeneity the lack a universal protein marker encourages use generic methods, including membrane labelling. Using nano‐flow cytometry, we evaluated...
Extracellular vesicles (EVs) are lipid bilayer structures released by all cells that mediate cell-to-cell communication via the transfer of bioactive cargo. Because natural origin EVs, their efficient uptake recipient cells, capacity to stabilize and transport biomolecules potential for cell/tissue targeting preferential cancer they have enormous bioengineering into improved targeted drug delivery systems. In this work, we investigated use laser ablation inductively coupled plasma mass...
Single particle characterization has become increasingly relevant for research into extracellular vesicles, progressing from bulk analysis techniques and first-generation to comprehensive multi-parameter measurements such as nano-flow cytometry (nFCM). nFCM is a form of flow that utilizes instrumentation specifically designed nano-particle analysis, allowing thousands EVs be characterized per minute both with without the use staining techniques. High resolution side scatter (SS) detection...
The development of novel screening tests aims to support early asymptomatic diagnosis and subtyping patients according similar traits in the heterogeneous cancer cohort. Extracellular vesicles (EVs) are promising candidates for detection disease markers from bodily fluids, but limitations standardisation isolation methods intrinsic EV heterogeneity obtained liquid biopsies currently obstacles clinical adoption. Here, cellular responses EVs were initially explored as potential complementary...
Abstract The proteomic profile of extracellular vesicles (EVs) from cerebrospinal fluid (CSF) can reveal novel biomarkers for diseases the brain. Here, we validate an ultrafiltration combined with size-exclusion chromatography (UF-SEC) method isolation EVs canine CSF and probe effect starting volume on EV proteomics profile. First, performed a literature review articles to define current state art, discovering need basic characterisation EVs. Secondly, isolated by UF-SEC characterised SEC...