A5003398236- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Synthetic Organic Chemistry Methods
- Clostridium difficile and Clostridium perfringens research
- Microbial metabolism and enzyme function
- Bacterial biofilms and quorum sensing
- Advanced biosensing and bioanalysis techniques
- Microbial Metabolism and Applications
- Oral microbiology and periodontitis research
- Synthesis and Biological Activity
- Plant-Microbe Interactions and Immunity
Chinese Academy of Sciences
2015-2023
Center for Excellence in Molecular Plant Sciences
2015-2023
University of Chinese Academy of Sciences
2020
Shanghai Institutes for Biological Sciences
2015
Abstract Quorum-sensing (QS) mediated dynamic regulation has emerged as an effective strategy for optimizing product titers in microbes. However, these QS-based circuits are often created on heterologous systems and require careful tuning via a tedious testing/optimization process. This hampers their application industrial Here, we design novel QS circuit by directly integrating endogenous system with CRISPRi (named EQCi) the rapamycin-producing strain Streptomyces rapamycinicus. EQCi...
Microbial utilization and conversion of organic one-carbon compounds, such as formate methanol that can be easily produced from CO2, has emerged an attractive approach for biorefinery. In this study, we discovered Clostridium beijerinckii NCIMB 8052, a typical solventogenic strain, to native formate-utilizing bacterium. 13C isotope analysis showed could metabolized via both assimilation dissimilation pathways in C. 8052. Notably, the use supplementary substrate by strain significantly...
Pristinamycin I (PI), produced by Streptomyces pristinaespiralis, is a streptogramin type B antibiotic, which contains two proteinogenic and five aproteinogenic amino acid precursors. PI coproduced with pristinamycin II (PII), member of A antibiotics. The biosynthetic gene cluster has been cloned characterized. However, thus far little understood about the regulation biosynthesis. In this study, TetR family regulator (encoded SSDG_03033) was identified as playing positive role in Its...
Gut microbes are closely related with human health, but remain much to learn.
ABSTRACT Dynamic regulation has emerged as an effective strategy to improve product titers by balancing metabolic networks, which can be implemented coupling gene expression pathway-independent regulatory elements, such quorum-sensing (QS) systems. However, these QS-based circuits are often created on heterologous systems and must carefully tuned through tedious testing optimization process make them work well, hampers their application in industrial microbes including streptomycetes. In...
Abstract Formic acid (FA), an organic one-carbon source that is easily produced from CO 2 , has emerged as a promising -equivalent feedstock for biorefinery. However, developing efficient formatotrophs economically competitive FA utilization system remains grand challenge. Here, we discovered the gram-negative bacterium Vibrio natriegens exceptional tolerance and metabolic capacity natively. This strain was then remodeled by rewiring serine cycle TCA which resulted in non-native closed loop...