A5031035044- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Virology and Viral Diseases
- Nanoparticles: synthesis and applications
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Parasitic Infections and Diagnostics
- Animal Virus Infections Studies
- Respiratory viral infections research
- Agriculture and Farm Safety
- Synthesis of Tetrazole Derivatives
- Rabies epidemiology and control
- Venomous Animal Envenomation and Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Inhalation and Respiratory Drug Delivery
- Diverse Scientific Research Studies
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Plant Pathogens and Resistance
- Cell death mechanisms and regulation
Agricultural Research & Education Organization
2017-2024
Razi Vaccine and Serum Research Institute
2011-2024
Institut de Recherche Vaccinale
2017
Nowadays, tuberculosis (TB) is one of the top ten leading causes mortality worldwide. The emergence multidrug-resistant (MDR) - and extensively drug-resistant (XDR) Mycobacterium (M. tuberculosis) identified as most challenging threats to TB control. Thus, new safe nano-drugs are urgently required for elimination TB. aim this study was investigate anti-bacterial effects Ag, ZnO, Ag-ZnO nanoparticles (NPs) on MDR- XDR-M. tuberculosis.In study, NPs were synthesized by chemical reduction...
Humans have been in a constant battle with tuberculosis (TB). Currently, overuse of antibiotics has resulted the spread multidrug-resistant Mycobacterium (MDR), leading to antibiotic ineffectiveness at controlling TB infection host cells and especially macrophages. Additionally, (Mtb) developed methods evade immune system survive. With discovery nanoparticle (NP)-based drugs, it is necessary research their anti-mycobacterial properties bactericidal mechanisms. In this study, we synthesized...
Abstract Background Small ruminant morbillivirus (SRMV) is the etiological agent of Peste des petits ruminants (PPR) disease. PPR one most important viral diseases small husbandry. In endemic countries, vaccination main way to control this Administering first vaccine in goat kids requires decreased maternal immunity. Objective The aim study was determine decreasing trend immunity against SRMV born from vaccinated goats. Methods Twenty Saanen were studied two groups including ( n = 5,...
The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes death worldwide. Nanotechnology and microbiology researchers are looking for new safe nano drugs eliminating Mycobacterium , causative agent tuberculosis. In this study, AgZnO nano‐crystals (AgZnONCs) synthesized via decomposition precursor oxalate method. Characterization AgZnONCs were evaluated. Next, various concentrations AgZnONCs, well AgZnONCs+Rifampicin,...
Abstract Background In goat kids, choosing the appropriate age to administer first dose of pox disease (GTP) vaccine requires knowing when maternal antibody decline concentrations. Objective Determine persistence antibodies against virus (GTPV) in kids. Animals Twenty Saanen kids from birth 120 days old. Methods 2 groups, including: control (receiving colostrum nonvaccinated does) and treatment vaccinated does). On zero, 3, 7, 14, 21, 28, 42, 56, 70, 100 after birth, neutralization test was...
The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3D pol ) coding regions foot mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (∼150 passages), was determined aligned with previously published FMDV serotype A sequences.Overall analysis amino acid substitutions revealed that region contained four alterations.Amino comparison field isolates deletions in Iranian (A/Iran87).The prominent G-H loop protein contains conserved arginine-glycine-aspartic...
Background:Envenomation by Macrovipera lebetina (M. lebetina) is characterized prominent local tissue damage, hemorrhage, abnormalities in the blood coagulation system, necrosis, and edema. However, main cause of death after a bite M. has been attributed to acute renal failure (ARF). It unclear whether venom components have direct or indirect action causing ARF. To investigate this point, we looked at vitro effect crude venom, using cultured human embryonic kidney (HEK-293) mono layers as...
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known with significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The IBD significantly cost-effective; however, it essential to confirm their safety efficacy. main cell line used product the vaccines, primary chicken embryo fibroblast (CEF). Nevertheless, manipulation CEF extremely challenging time-consuming. This study aimed characterize sensitive...
The A Iran 05 foot-and-mouth disease virus (FMDV) subtype was detected in during 2005 and has proven to be highly virulent.This study undertaken focus on molecular phylogenetic analysis of 3A 3B coding-regions the field isolate.To assess genetic relatedness isolate nucleotide predicted amino acid sequences 3AB region type FMDV isolates were compared with twenty previously described isolates.The tree based 672 bp gene from thirteen different locations clustered them into five distinct...
Preparation and Characterization of a Neutralizing Monoclonal Antibody against Poliovirus Type 1 (Mahoney Strain)
Monoclonal Antibodies (mAbs) are used for biomedical research, diagnosis, treatment, production, and the quality control of biological products. mAbs also very helpful in poliovirus research studies because it is still one major public health problems developing countries. The main objective this study was production against Poliovirus Type 2 (PV2) to be prepared respond re-emergence virus. After fusion immunized B cells from mice with myeloma tumor screening about 250 hybridoma colonies, 22...
Diploid and continuous cell lines are used to propagate viral vaccines. At Human Viral Vaccine Department of Razi Serum Research Institute, MRC5 diploid is for the development live attenuated measles, mumps, rubella, three types poliovirus Additionally, (i.e., RK13, HeLa, Vero) applied in quality control tests. Accordingly, cross-contamination can occur at culture labs, hence controlling identity specificity cells essential. Indirect immunofluorescence a sensitive, specific, simple test...
The production of purified virus particles with high quality and quantity for vaccine preparation requires scalable purification procedure in downstream step. A scheme based on combined strong anion-exchange size exclusion chromatography (2D -AEC×SEC) is developed non-structural protein (NSP) free foot mouth diseases (FMD) the whole accomplished 78 % recovery, 85 yield more than 90 residual DNA (rDNA) removed from vaccine. Due to use AEC as first column, injection volume increases four times...