A5085914964- Primary Care and Health Outcomes
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Healthcare Systems and Practices
- Herpesvirus Infections and Treatments
- Kruppel-like factors research
- Virus-based gene therapy research
- Global Health Workforce Issues
- Acute Myeloid Leukemia Research
- Health, Medicine and Society
- Healthcare Systems and Technology
- Diabetic Foot Ulcer Assessment and Management
- Tuberous Sclerosis Complex Research
- Polyomavirus and related diseases
- Chronic Myeloid Leukemia Treatments
- Nursing Roles and Practices
- Hemoglobinopathies and Related Disorders
- Wound Healing and Treatments
- Clinical practice guidelines implementation
- Healthcare Policy and Management
- Effects of Environmental Stressors on Livestock
- Cytomegalovirus and herpesvirus research
- Poxvirus research and outbreaks
- Multiple Myeloma Research and Treatments
- Veterinary Equine Medical Research
- Health Sciences Research and Education
University of Saskatchewan
2017-2024
Queen Elizabeth II Health Sciences Centre
2005-2023
Canadian Association of Emergency Physicians
2023
North York General Hospital
2023
University of Ottawa
2023
Ottawa Hospital
2023
Saskatoon Medical Imaging
2019
Cambridge University Hospitals NHS Foundation Trust
2018
University of Cambridge
2011-2018
Stem Cell Institute
2018
Somatic mutations in the Janus kinase 2 gene (JAK2) occur many myeloproliferative neoplasms, but molecular pathogenesis of neoplasms with nonmutated JAK2 is obscure, and diagnosis these remains a challenge.
Myeloproliferative neoplasms, such as polycythemia vera, essential thrombocythemia, and myelofibrosis, are chronic hematologic cancers with varied progression rates. The genomic characterization of patients myeloproliferative neoplasms offers the potential for personalized diagnosis, risk stratification, treatment.
Clonal proliferation in myeloproliferative neoplasms (MPN) is driven by somatic mutations JAK2, CALR or MPL, but the contribution of inherited factors poorly characterized. Using a three-stage genome-wide association study 3,437 MPN cases and 10,083 controls, we identify two SNPs with significance JAK2(V617F)-negative MPN: rs12339666 (JAK2; meta-analysis P=1.27 × 10(-10)) rs2201862 (MECOM; P=1.96 10(-9)). Two additional SNPs, rs2736100 (TERT) rs9376092 (HBS1L/MYB), achieve when including...
<h3>Aims</h3> The Tuberous Sclerosis 2000 Study is the first comprehensive longitudinal study of tuberous sclerosis (TS) and aims to identify factors that determine prognosis. Mode presentation findings at initial assessments are reported here. <h3>Methods</h3> Children aged 0–16 years newly diagnosed with TS in UK were evaluated. <h3>Results</h3> 125 children studied. 114 (91%) met clinical criteria for a definite diagnosis remaining 11 (9%) had pathogenic <i>TSC1</i> or <i>TSC2</i>...
The herpes simplex virus type 1 genome contains four open reading frames (ORFs) which are predicted to encode hydrophobic proteins with the potential cross a membrane several times. products of these genes (genes UL10, UL20, UL43 and UL53) have not previously been identified. To investigate role in life cycle, we attempted inactivate individually by inserting lacZ gene from Escherichia coli within ORFs. Using this approach isolated insertion mutants for UL10 UL43, as well deletion mutant...
Purpose Cytoreductive therapy is beneficial in patients with essential thrombocythemia (ET) at high risk of thrombosis. However, its value those lacking high-risk features remains unknown. This open-label, randomized trial compared hydroxycarbamide plus aspirin alone ET age 40 to 59 years and without factors or extreme thrombocytosis. Patients Methods were lacked a history ischemia, thrombosis, embolism, hemorrhage, thrombocytosis (platelet count ≥ 1,500 × 10 9 /L), hypertension, diabetes...
<h3>Background:</h3> Comprehensive diagnostic imaging referral guidelines are an important tool to assist referring clinicians and radiologists in determining the safest best-clinical-value study for their patients; Canadian Association of Radiologists (CAR) last produced its 2012. In partnership with several national organizations, clinicians, radiologists, patient family advisors from across Canada, association is redoing using a new methodology guideline development, these recommendations...
Background Tuberous sclerosis complex (TSC) is associated with intellectual disability, but the risk pathways are poorly understood. Method The Sclerosis 2000 Study a prospective longitudinal study of natural history TSC. One hundred and twenty-five UK children age 0–16 years TSC born between January 2001 December 2006 were studied. Intelligence was assessed using standardized measures at ≥2 age. onset epilepsy, type seizure disorder, frequency duration seizures, as well response to...
Infantile spasms (IS) have long been suspected to be a risk factor for impairment in intellectual development, but there are no controlled, prospective longitudinal data well-characterized conditions confirm this suspicion. We tested the hypothesis study of children with tuberous sclerosis (TS), who high developing IS.Eleven infants TS were recruited and studied longitudinally using Mullen Scales Early Learning. Seizure histories assessed structured parent interview by review medical notes....
Introduction Sleep-time blood pressure correlates more strongly with adverse cardiovascular events than does daytime pressure. The BedMed trial evaluates whether bedtime antihypertensive administration, as compared conventional morning use, reduces major events. Methods and analysis Design Prospective randomised, open-label, blinded end-point trial. Participants Hypertensive primary care patients using lowering medication free from glaucoma. Setting Community providers in 5 Canadian...
We have investigated whether herpes simplex virus (HSV) contains structural polypeptides which are modified by myristic acid. demonstrate that virions contain a family of myristylated proteins, Mr approximately 13,000 to 16,000. These were mapped, using HSV-1/HSV-2 intertypic recombinants, 0.130 0.204 map units on the genome. Using anti-peptide sera, raised against carboxyterminus predicted UL11 gene product, we established virion products viral UL11.
On the basis of predicted amino acid sequence characteristics, herpes simplex virus type 1 gene UL10 is thought likely to encode a membrane protein with eight potential transmembrane regions. Previously, an apparent M(r) 47,000 on SDS-PAGE was identified as product this gene. Here we have further characterized protein, and show that it modified by N-linked glycosylation, associates membranes from infected cells, component particle. It not essential for growth in tissue culture. To...
Herpes simplex virus type 1 (HSV-1) gene UL11 encodes a myristylated virion protein. In this paper we have characterized the product further and investigated its role in life cycle. Wild-type HSV-1 strain 17syn+ expresses three electrophoretically distinguishable polypeptide species. Analysis of single plaque isolates demonstrated that two populations exist within stock: major population encoding only higher Mr species, minor lowest species alone. DNA sequence analysis suggests latter...
Headaches are prevalent among teens and young adults. Self-monitoring is essential for managing headaches can be accomplished with the help of electronic headache diaries. An increasing number diaries exist, yet absence quality standards compromises their use research clinical purposes.Our goal was to develop test usability, feasibility, psychometric properties an diary iPhone application self-monitoring by adolescents adults headaches.We used iterative participatory design our diary....
We have used antisera raised against synthetic oligopeptides to characterize the protein products from herpes simplex virus type 1 gene US11. These recognized predominantly polypeptides of apparent molecular weight 21,000 and 22,000, but also 17,500, 15,000, 14,000 11,000. Tryptic peptide fingerprint analysis confirmed that these were all closely related. The 22,000 shown be DNA-binding proteins, immune electron microscopy demonstrated their strong localization within nucleoli infected cells.
Studies have shown that early enteral nutrition in critically ill patients reduces the incidence of morbidity and death. Nonetheless, intolerance to gastric is common these as a result gastroparesis. The use prokinetic agents such metoclopramide, domperidone, cisapride, erythromycin can improve emptying, but are not without deleterious adverse effects. Tegaserod, selective serotonin type 4 receptor partial agonist, was recently approved for treatment women with irritable bowel syndrome. On...
Herpes simplex virus type 1 (HSV-1) gene UL49A potentially encodes a primary translation product of 91 residues with signal sequence at the N terminus and membrane anchor domain near C terminus. Mutants were generated in this utilized to characterize encoded protein on SDS-PAGE as 6·7 kDa species which fractionated infected cell membranes, was relatively abundant virion component, not detectably O-glycosylated. The identified by microsequencing 68 residue polypeptide formed removal 23 from...
In 2009, Yvonne Steinert et al., at McGill University, published a study exploring barriers to faculty development (FD) participation among urban faculty. Over decade later, we set out replicate and expand on that learn what has changed in continued professional (CPD) the current are CPD for specialists family physicians rural locations.