A5040539722- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Supramolecular Self-Assembly in Materials
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- DNA Repair Mechanisms
- Viral Infections and Immunology Research
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Electrohydrodynamics and Fluid Dynamics
- RNA Interference and Gene Delivery
- Insect and Arachnid Ecology and Behavior
- Graphene and Nanomaterials Applications
- Invertebrate Immune Response Mechanisms
- Fungal and yeast genetics research
- COVID-19 Clinical Research Studies
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Adipokines, Inflammation, and Metabolic Diseases
- Chromosomal and Genetic Variations
- Insect and Pesticide Research
- Viral Infections and Vectors
- Telomeres, Telomerase, and Senescence
Tulane University
2014-2024
National Institute of Allergy and Infectious Diseases
2023-2024
National Institutes of Health
2024
Tula University
2020
University Medical Center New Orleans
2014-2017
Enterovirus D68 (EV-D68) causes severe respiratory illness in children and can result a debilitating paralytic disease known as acute flaccid myelitis. No treatment or vaccine for EV-D68 infection is available. Here, we demonstrate that virus-like particle (VLP) vaccines elicit protective neutralizing antibody against homologous heterologous subclades. VLP based on B1 subclade 2014 outbreak strain elicited comparable activity an inactivated viral mice. Both immunogens weaker...
Human enteroviruses are the most common human pathogen with over 300 distinct genotypes. Previous work poliovirus has suggested that it is possible to generate antibody responses in humans and animals can recognize members of multiple enterovirus species. However, cross protective immunity across not observed epidemiologically humans. Here we investigated whether immunization mice or baboons inactivated virus-like-particles (VLPs) vaccines generates D68 A71. We found only generated...
The function of the replication clamp loaders in semi-conservative telomere and their relationship to telomerase- recombination mechanisms addition remains ambiguous. We have investigated variant loader Ctf18 RFC (Replication Factor C). To understand role at telomere, we first genetic interactions after loss TLC1 (the yeast telomerase RNA). find that tlc1Δ ctf18Δ double mutant confers a rapid >1000-fold decrease viability. rate was similar kinetics cell death rad52Δ cells. However, pathway...
Antigen processing in the class II MHC pathway depends on conventional proteolytic enzymes, potentially acting antigens native-like conformational states. CD4+ epitope dominance arises from a competition among antigen folding, proteolysis, and MHCII binding. Protease-sensitive sites, linear antibody epitopes, T-cell epitopes were mapped plague vaccine candidate F1-V to evaluate various contributions dominance. Using X-ray crystal structures, likelihood (APL) predicts with significant...
The emergence of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and the subsequent COVID-19 pandemic have visited a terrible cost on world in forms disease, death, economic turmoil. rapid development deployment extremely effective vaccines against SARS-CoV-2 seemingly brought within reach end pandemic. However, virus has acquired mutations. emerging variants concern are more infectious reduce efficacy existing vaccines. Although promising efforts to combat these...
Chicken ovalbumin (cOVA) has been studied for decades primarily due to the robust genetic and molecular resources that are available experimental investigations. cOVA is a member of serpin superfamily proteins function as protease inhibitors, although does not exhibit this activity. As serpin, possesses protease-sensitive reactive center loop lies adjacent OVA 323–339 CD4+ T-cell epitope. We took advantage previously described single-substitution variant, R339T, which can undergo dramatic...
The synthetically evolved pHD family of peptides is known to self-assemble into macromolecule-sized nanopores 2-10 nm diameter in synthetic lipid bilayers, but only when the pH below ∼6. Here, we show that a representative member, pHD108, has same pH-responsive nanopore-forming activity endosomal membranes living human cells, which triggered by acidification. This enables cytosolic delivery endocytosed proteins and other macromolecules. Acylation either peptide terminus significantly...
Telomeres, the nucleoprotein complexes at termini of linear chromosomes, are essential for processes end replication, protection, and chromatin segregation. The Mre11 complex is involved in multiple cellular roles DNA repair structure regulation function telomere size homeostasis. In this study, we characterize yeast structure, phenotypic heritability, segregation both wild-type [MRE11] A470 motif alleles. MRE11 strains confer a 300 base pairs G+T irregular simple sequence repeats. This...
Abstract The emergence of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and the subsequent COVID-19 pandemic has visited a terrible cost on world in forms disease, death, economic turmoil. rapid development deployment extremely effective vaccines against SARS-CoV-2 have seemingly brought within reach end pandemic. However, virus acquired mutations; emerging variants concern (VOC) are more infectious reduce efficacy existing vaccines. While promising efforts to combat...
Abstract Antigen processing in the class II MHC pathway depends on conventional proteolytic enzymes, potentially acting antigens native-like conformational states. CD4+ epitope dominance arises from a competition between antigen folding, proteolysis, and MHCII binding. Protease-sensitive sites, linear antibody epitopes, T-cell epitopes were mapped plague vaccine candidate F1-V to evaluate various contributions dominance. Using X-ray crystal structures, likelihood (APL) predicts with...
Abstract Potent antibody responses depend on the proteolytic processing of protein antigens and presentation MHC Class II bound peptides to CD4+ T cells. We have previously reported a single amino acid substitution (R494A) made pseudomonas exotoxin A domain III (PE-III) that alters with only minor changes T-cell priming. These findings could not explain how R494A causes substantial reduction in titer. Previous work by our laboratory others has shown stability affects priming through effects...