A5075871825- Bacteriophages and microbial interactions
- Advanced NMR Techniques and Applications
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Chemical Reactions and Isotopes
- Mass Spectrometry Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Muon and positron interactions and applications
- Advanced MRI Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- Cardiomyopathy and Myosin Studies
- Solid-state spectroscopy and crystallography
- Lanthanide and Transition Metal Complexes
- Microbial Community Ecology and Physiology
- Ion channel regulation and function
- Metal complexes synthesis and properties
- RNA Research and Splicing
- Muscle Physiology and Disorders
CEA Grenoble
2017-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2025
Université Grenoble Alpes
2017-2025
Institut de Biologie Structurale
2017-2025
Centre National de la Recherche Scientifique
2017-2025
HMU Potsdam – Health and Medical University Potsdam
2024
MSB Medical School Berlin
2024
University of Potsdam
2024
The vast majority of phages, bacterial viruses, possess a tail ensuring host recognition, cell wall perforation and safe viral DNA transfer from the capsid to cytoplasm. Long flexible tails are formed tube protein (TTP) polymerised as hexameric rings around stacked along tape measure (TMP). Here, we report crystal structure T5 TTP pb6 at 2.2 Å resolution. Pb6 is unusual in forming trimeric ring, although analysis reveals homology with all classical TTPs related proteins puncturing devices...
Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to infected bacterium cytoplasm. The majority of tailed phages bear long flexible ( Siphoviridae ) at tip which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, unique RBP is located extremity central fiber. We structures T5 tip, determined by cryo–electron microscopy before after interaction its E. coli...
Introducing an unprecedented nucleating and phasing agent for protein crystallography that presents convenient luminescence properties.
Abstract Solid‐state NMR spectroscopy can provide insight into protein structure and dynamics at the atomic level without inherent size limitations. However, a major hurdle to studying large proteins by solid‐state is related spectral complexity resonance overlap, which increase with molecular weight severely hamper assignment process. Here use of two sets experiments shown expand tool kit 1 H‐detected approaches, correlate given amide pair either adjacent CO–CA pairs (4D hCOCANH/hCOCAcoNH),...
Initially, the two members of class 18 myosins, Myo18A and Myo18B, appeared to exhibit highly divergent functions, complicating assignment class-specific functions. However, identification a striated muscle-specific isoform Myo18A, Myo18Aγ, suggests that myosins may have evolved complement functions conventional 2 in sarcomeres. Indeed, both genes,
Abstract. Amide-proton detected magic-angle spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D2O-based culture media, non-exchangeable amide sites remain deuterated, making these unobservable. Here we demonstrate produced with H2O-based medium doped cell lysate allow to overcome this "reprotonation bottleneck", while retaining high level (ca. 80 %) and narrow line widths. We quantified coherence...
Abstract. Amide-proton-detected magic-angle-spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D2O-based culture media, non-exchangeable amide sites remain deuterated, making these unobservable. Here we demonstrate produced with H2O-based medium doped cell lysate allow scientists to overcome this “reprotonation bottleneck” while retaining high level (ca. 80 %) and narrow linewidths. We quantified...
Abstract The vast majority of bacteriophages (phages) - bacterial viruses present a tail that allows host recognition, cell wall perforation and safe channelling the viral DNA from capsid to cytoplasm infected bacterium. tailed phages bears long flexible ( Siphoviridae ) at distal end which tip complex, often called baseplate, harbours one or more Receptor Binding Protein·s (RBPs). Interaction between RBPs surface triggers ejection, but little is known on these mechanisms for . Here, we...
Amide-proton detected magic-angle spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D 2 O-based culture media, non-exchangeable amide sites remain deuterated, making these unobservable. Here we demonstrate produced with H medium doped cell lysate allow to overcome this “reprotonation bottleneck”, while retaining high level (ca. 80 %) and narrow line widths. We quantified coherence life times several...