A5071117583- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Skin Protection and Aging
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Skin and Cellular Biology Research
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- interferon and immune responses
- Wound Healing and Treatments
- DNA Repair Mechanisms
- Nonmelanoma Skin Cancer Studies
- Psoriasis: Treatment and Pathogenesis
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- melanin and skin pigmentation
- Dermatology and Skin Diseases
- Hedgehog Signaling Pathway Studies
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
- TGF-β signaling in diseases
Oregon Health & Science University
2013-2024
Pfizer (United Kingdom)
2022
Sanofi (Mexico)
2022
Regeneron (United States)
2022
Estée Lauder (United States)
2022
Bristol-Myers Squibb (Germany)
2022
Janssen (Belgium)
2022
Sanofi (France)
2022
LEO Foundation
2022
Carnegie Endowment for International Peace
2022
Here, we describe a multiplexed immunohistochemical platform with computational image processing workflows, including cytometry, enabling simultaneous evaluation of 12 biomarkers in one formalin-fixed paraffin-embedded tissue section. To validate this platform, used microarrays containing 38 archival head and neck squamous cell carcinomas revealed differential immune profiles based on lymphoid myeloid densities, correlating human papilloma virus status prognosis. Based these results,...
An mRNA differential display comparison of mouse JB6 promotion-sensitive (P+) and -resistant (P−) cells identified a novel gene product that inhibits neoplastic transformation. The P+ P− are genetic variants differ in their transformation response to tumor promoters; form anchorage-independent colonies tumorigenic, do not. A differentially displayed fragment, A7-1 , was preferentially expressed at levels ≥10-fold those cells, making its candidate inhibitor cDNA isolated identical murine...
TGF-β and its signaling mediators, Smad2, -3, -4, are involved with tumor suppression promotion functions. Smad4–/– mouse epidermis develops spontaneous skin squamous cell carcinomas (SCCs), Smad3–/– mice resistant to carcinogen-induced cancer; however, the role of Smad2 in carcinogenesis has not been explored. In present study, we found that Smad4, but Smad3, were frequently lost human SCCs. Mice keratinocyte-specific deletion exhibited accelerated formation malignant progression chemically...
Abstract In the present study, we show that transforming growth factor β1 (TGF-β1) was frequently overexpressed in human head and neck squamous cell carcinomas (HNSCCs) adjacent tissues comparison with normal tissues. To determine role of TGF-β1 overexpression HNSCC carcinogenesis, generated transgenic mice which transgene expression can be induced epithelia. induction epithelia, at levels similar to those HNSCCs, caused severe inflammation angiogenesis. Consequently, TGF-β1-transgenic...
Muscular dystrophy-associated mutations of TRIM32 impair ULK1-dependent autophagy response during muscle atrophy.
Exposure to UV light contributes the development of skin cancer. The importance reactive oxygen species in UV-radiation carcinogenesis has been recognized for some time and several associated DNA base modifications have identified. In particular, 8-hydroxydeoxyguanosine (8-OHdG) well studied as an indicator oxidative damage calf thymus exposed a variety oxygen-generating systems, including light. However, date, few studies 8-OHdG conducted cell or animal systems those vitro investigations...
Eight cell lines exhibiting resistance to Ca 2+ induced terminal differentiation were derived from primary mouse epidermal cultures and their properties analyzed. The developed either spontaneously (2 lines) or after exposure of carcinogens tumor promoter. All but one the epithelial epitheloid morphology 3 8 lacked desmosomes, keratin filaments immunoprecipitable proteins, thus could not be defined as keratinocytes. Two 5 keratino-cyte tumorigenic in syngeneic Balb/c newborns 4 months medium...
A body of research demonstrates examples in vitro and vivo synergy between natural products anti-neoplastic drugs for some cancers. However, the underlying biological mechanisms are still elusive. To better understand entities targeted by therefore provide rational evidence future novel combination therapies cancer treatment, we assess targetable space using public domain compound-target information. When considering pathways from Reactome database products, found an increase coverage 61%...
Tripartite motif protein 32, Trim32, mRNA and expression was elevated in independently transformed tumorigenic keratinocytes of a mouse epidermal carcinogenesis model, ultraviolet B (UVB)-induced squamous cell carcinomas (SCC), approximately 20-25% chemically induced papillomas human head neck SCCs. This suggests that Trim32 occurs frequently experimental is relevant to cancer. Transduced increased colony number an vitro transformation assay thickening vivo when skin-grafted athymic nu/nu...
Basal epidermal cells can be selectively maintained as a monolayer in culture medium containing low ionic calcium concentration of 0.01 – 0.10 mM. Cessation proliferation, maturation and shedding squamous sheets induced this population by increasing the above 0.1 Since alterations regulation proliferation differentiation are associated with carcinogenesis vivo, it appeared reasonable that changes phenotypic response to might follow exposure carcinogens vitro. Support for hypothesis was...
Negative regulation of the NF-κB transcription factor is essential for tissue homeostasis in response to stress and inflammation. activity regulated by a variety biochemical mechanisms including phosphorylation, acetylation, ubiquitination. In this study, we provide first experimental evidence that SUMOylation, where RelA subunit SUMOylated PIAS3, member PIAS (protein inhibitor activated STAT) protein family with E3 SUMO ligase activity. PIAS3-mediated repression was compromised either...
Head and neck squamous cell carcinoma (HNSCC) patients have a poor prognosis, with invasion metastasis as major causes of mortality. The phosphatidylinositol 3-kinase (PI3K) pathway regulates wide range cellular processes crucial for tumorigenesis, PIK3CA amplification mutation are among the most common genetic alterations in human HNSCC. Compared well-documented roles PI3K growth survival, tumor not been well delineated. We generated genetically engineered mouse model (PIK3CA-GEMM) which...
Protein inhibitors of activated STATs (PIAS) family members are ubiquitin-protein isopeptide ligase-small ubiquitin-like modifier ligases for diverse transcription factors. However, the regulation PIAS protein activity in cells is poorly understood. Previously, we reported that expression Trim32, a RING domain ligase-ubiquitin ligase mutated human limb-girdle muscular dystrophy type 2H (LGMD2H) and Bardet-Biedl syndrome, elevated during mouse skin carcinogenesis, protecting keratinocytes...
Optical elastography is an imaging modality that relies on variations in the local mechanical properties of biological tissues as contrast mechanism for image formation. Skin lesions, such melanomas and other invasive conditions, are known to alter arrangement collagen fibers skin thus should lead alterations properties. We report acousto-optical (AOE) quantifying behavior lesions. The method upon stimulating tissue with a low frequency acoustic force resulting strains by means magnitude...
We previously demonstrated that a wild-type alternatively spliced p53 (p53as) RNA exists in mouse cultured cells and normal tissues at approximately 25 to 33% of the level major form. The alternative transcript is 96 nucleotides longer than as result splicing intron 10 sequences. protein expected be generated from p53as 9 amino acids shorter has 17 different carboxyl terminus. report here nontransformed malignant epidermal localized nucleus. In addition, preferentially expressed during G2...
Abstract Background Microarray technology has become very popular for globally evaluating gene expression in biological samples. However, non-linear variation associated with the can make data interpretation unreliable. Therefore, methods to correct this kind of technical are critical. Here we consider a method reduce type applied after three common procedures processing microarray data: MAS 5.0, RMA, and dChip ® . Results We commonly observe intensity-dependent between samples single...
The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level regularly p53 in both normal epidermal and carcinoma cells. presence this 10T1/2 fibroblast cells, mouse liver testis suggests that alternative splicing may be universal. was increased coordinatety with cells response growth factor. Immunoprecipitation analysis using monoclonal antibodies which recognize different...