A5046492087- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Veterinary medicine and infectious diseases
- interferon and immune responses
- Influenza Virus Research Studies
- Respiratory viral infections research
- 14-3-3 protein interactions
- Toxin Mechanisms and Immunotoxins
- Bioinformatics and Genomic Networks
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Genetic Mapping and Diversity in Plants and Animals
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Fungal and yeast genetics research
- Biochemical and Molecular Research
- Coccidia and coccidiosis research
- Genomics and Rare Diseases
University of Saskatchewan
2013-2019
College of Law
2019
Western University of Health Sciences
2014
Institute of Bioinformatics
2003
Human Protein Reference Database (HPRD) is an object database that integrates a wealth of information relevant to the function human proteins in health and disease. Data pertaining thousands protein-protein interactions, posttranslational modifications, enzyme/substrate relationships, disease associations, tissue expression, subcellular localization were extracted from literature for nonredundant set 2750 proteins. Almost all was obtained manually by biologists who read interpreted >300,000...
Lawsonia intracellularis (LI) are obligate intracellular bacteria and the causative agent of proliferative hemorrhagic enteropathy that significantly impacts health piglets profitability swine industry. In this study, we used immunoinformatic computational methodologies such as homology modelling, molecular docking, dynamic (MD) simulation, free energy calculations in a novel three stage approach to identify strong T B cell epitopes LI proteome. From ∼ 1342 proteins, narrowed our focus 256...
We present a novel small molecule antiviral chemotype that was identified by an unconventional cell-free protein synthesis and assembly-based phenotypic screen for modulation of viral capsid assembly. Activity PAV-431, representative compound from the series, has been validated against infectious viruses in multiple cell culture models all six families causing most respiratory diseases humans. In animals, this demonstrated efficacious porcine epidemic diarrhoea virus (a coronavirus)...
Viruses modulate the functions of mitochondria by translocating viral proteins to mitochondria. Subcellular fractionation and sensitivity proteinase K/Triton X-100 treatment mitochondrial fractions bovine adenovirus (BAdV)-3-infected/transfected cells suggested that core protein pVII localizes contains a functional localization signal. Moreover, BAdV-3 appears help in retention Ca 2+ , inducing significant increase levels ATP maintaining membrane potential (MMP) transfected cells. In...
Two structurally unrelated small molecule chemotypes, represented by compounds PAV-617 and PAV-951, with antiviral activity in cell culture against Mpox virus (formerly known as monkeypox virus) human immunodeficiency (HIV) respectively, were studied for anti-cancer efficacy. Each exhibited apparent pan-cancer cytotoxicity reasonable pharmacokinetics. Non-toxicity is demonstrated a non-cancer line mice at doses achieving drug exposure active concentrations. Anti-tumour properties of both...
Abstract We present a novel small molecule antiviral chemotype that was identified by an unconventional cell-free protein synthesis and assembly-based phenotypic screen for modulation of viral capsid assembly. Activity PAV-431, representative compound from the series, has been validated against infectious virus in multiple cell culture models all six families viruses causing most respiratory disease humans. In animals this demonstrated efficacious Porcine Epidemic Diarrhea Virus (a...
Viruses alter the structure and function of mitochondria for survival. Electron microscopy analysis cells infected with bovine adenovirus 3 revealed extensive damage to inner mitochondrial membrane characterized by dissolution cristae amorphous appearance matrix little or no outer membrane. There were fewer altered morphology. Potential patches protein synthesis machinary around could be observed at 12 hours post infection (hpi). At 24 hpi, multi vascular bodies evident throughout cell. ATP...