Salmonella Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize S. strains production. this study, 87 were isolated from chicken meat for sale supermarkets Santiago, characterized according their virulence genes, biofilm formation abilities, antibiotic susceptibility, resistance genes. Through polymerase chain reaction or PCR, the analyzed detect presence of 11 12 integrase...

The aim of this research was to statistically analyze the association between antimicrobial susceptibility/resistance erythromycine, gentamicin, ciprofloxacin, and tetracycline 11 virulence genes associated with adherence, invasion, cytotoxicity in 528 isolates Campylobacter coli jejuni obtained from retail meat fecal samples food-producing animals human patients. A high percentage strains were resistant antimicrobials, specifically ciprofloxacin tetracycline. Moreover, we observed a wide...

Mesenchymal stem cells (MSC) are multipotent progenitor characterized by their ability to both self-renew and differentiate into tissues of mesodermal origin. The plasticity or transdifferentiation potential MSC is not limited derivatives, since under appropriate cell culture conditions stimulation bioactive factors, have also been differentiated endodermal (hepatocytes) neuroectodermal (neurons) cells. for hepatogenic neurogenic differentiation has well documented in different animal...

Salmonella enterica is an important foodborne pathogen. Commercial poultry are the main reservoirs of enterica, leading to contamination food and outbreaks in humans. The vaccination chickens one most strategies reduce number farms. Unfortunately, commercial vaccines have not been fully effective controlling spread do contain all serovars that circulate on In this study, we evaluate a new, cochleate-based, trivalent injectable vaccine against S. Enteritidis, Typhimurium Infantis, describing...

Contradictory data have been reported regarding the effect of GnRH agonists and antagonists on cell growth survival, using prostate cancer-derived lines expressing either endogenous or exogenous receptors. We addressed issue studying leuprolide (agonist) cetrorelix (antagonist) growth, apoptosis receptor expression a primary coculture system. Also, binding characteristics in this culture system are described. Epithelial stromal cells were obtained from adenocarcinoma samples cocultured...

Abstract Echinococcus granulosus , the agent of hydatid disease, presents an indirect life cycle, with canines (mainly dogs) as definitive hosts, and herbivores human intermediary ones. In hosts fertile infertile cysts develop, but only first ones develop protoscoleces, parasite form infective to hosts. We report presence bovine IgGs in germinal layer from (GLIC), order magnitude greater than (GLFC). When extracted salt solutions, GLIC are associated low or high affinity (most likely...

Leptospirosis is an important zoonosis with worldwide distribution caused by pathogenic bacteria of the genus Leptospira. The North American mink (Neovison vison) has role in environmental contamination Leptospira, as minks live aquatic environments and are predators rodents. Blood kidney samples were obtained from 57 Southern Chile 39° S to 45° S. Pathogenic species Leptospira detected PCR on 31/57 minks. To determine species, we sequenced 16S ribosomal RNA (rRNA) gene nine positive...

Cystic echinococcosis is a worldwide zoonosis caused by the cestode Echinococcus granulosus. Two types of hydatid cysts occur in intermediate hosts: fertile that generate protoscoleces from germinal layer cyst, and infertile do not produce are unable to continue life cycle parasite. The adventitial layer, host-derived fibrous capsule surrounding suggested play an important role local immune regulation during infection fertility cysts. Fasciola hepatica, another parasite cattle, induces...

Canine parvovirus (CPV) is a major pathogen in canines, with high mortality rate unvaccinated puppies. CPV traditionally classified into three antigenic variants (CPV-2a, CPV-2b and CPV-2c) based on the amino acid sequence of VP2 protein. Currently, various mutations are described receptor-binding area or regions greatest antigenicity protein, giving rise to new viral that capable immunological escape, affecting protective immunity traditional vaccines. In present study, molecular...

The introduction of the 4/91 vaccine against infectious bronchitis in Chile, a lineage not described until that time country, led to looking for changes induced by this action. This study considers eight isolates obtained from 2009, 2015 and 2017 uses maximum likelihood approach classify field isolates. Three were selected analyze antigenic relationships through virus neutralization test perform protection tests measured trough an RT-qPCR. 2009 showed relationship with GI-16 while those...

Reproductive control in mammals, particularly beef production systems, is crucial for managing behaviors driven by sex steroids, which can cause biosecurity issues, reduced weight gain, and decreased meat quality. Additionally, controlling sexual activity females prevents unwanted pregnancies when both sexes are housed together fattening. Surgical castration cattle, commonly performed under field conditions, associated with significant welfare concerns due to pain infection risks....

Summary We investigate the proliferative activity, prostatic specific antigen (PSA) secretion, morphology and androgen response of human prostate tumour epithelial cells co‐cultured with stromal in a bicameral system. Stromal were isolated from adenocarcinoma by enzyme digestion cultured defined media. Immunocytochemistry for carcinoma (PCTA‐1) was performed culture purity evaluation. Also, co‐culture evaluated electron microscopy. PSA determined microparticle immunoassay (MEIA) automatized...

The specific signaling connections between the mitogen-activated protein kinases (MAPK) such as c-Jun Nterminal kinase (JNK-1) and phosphatases PP4 M3/6, affecting family of early nuclear factors, is complex remains poorly understood.JNK-1 regulates cellular differentiation, apoptosis stress responsiveness by up-regulating factors c-Jun, a member activating (AP-1) family, Early Growth Factor (EGR-1).C-Jun, when phosphorylated N-terminal associates with c-Fos to form AP-1 transcription factor...

Salmonella enterica is a food-borne pathogen with wide host-range that during decades has been of public health concern in developed and developing countries. In Chile, the poultry pig industries represent biggest contribution meat consumption population, sanitary regulations have imposed for control. The aim this work was to determine characterize strains isolated from pigs chicken raised on commercials farms Chile. For this, isolates belonging (n = 46) 57) were genotyped by two multiplex...

The use of extracellular matrix (ECM) molecules from tissues is an interesting way to induce specific responses cells grown onto composite scaffolds promote adhesion, proliferation and differentiation. There have been several studies on the effects cell differentiation osteoprogenitor cultured composites, either adding some ECM or in presence growth factors. Other involve osteoblasts a three-dimensional (3D) matrix, enriched with produced by same previously inside composite. Here, effect...

Canine immunocastration development has been of interest for many years as a complementary strategy to surgical castration. The purpose this paper was verify the effect recombinant vaccine dog immunocastration. Two tests were done, one under controlled conditions and second field conditions. Animals injected with 1 mL 500 µg GnRXG/Q protein; low molecular weight chitosan adjuvant; NaCl 0.9% q.s. In first trial, eight Beagle male dogs between ages 3 comprised sample, randomly divided into two...

Cystic Echinococcosis (CE), a zoonotic parasitic disease, is caused by the cestode Echinococcus granulosus sensu lato. CE inflicts severe damage in cattle, sheep, and human hosts worldwide. Fertile cysts are characterized presence of viable protoscoleces. These parasite forms studied with minimal contamination host molecules. Hosts, cattle show differences their cyst fertility. The effect protoscolex transcriptome not known. We genotyped performed transcriptomic analysis on sheep...

Abstract Salmonid rickettsial septicaemia (SRS) is a contagious disease caused by Piscirickettsia salmonis , an intracellular bacterium. SRS causes estimated economic loss of $700 million USD to the Chilean industry annually. Vaccination and antibiotic therapy are primary prophylactic control measures used against SRS. Unfortunately, commercially available vaccines have not been shown significant effect on reducing mortality. Most contain whole inactivated bacteria which results in decreased...

Problem Immunocastration or vaccination against the GnRH‐I hormone is a promising alternative to reproductive control in different animal species. Given low immunogenicity of this hormone, use adjuvants becomes necessary. Method Study This study evaluated effects three that induce immune response profiles over gonadal function, fertility, and expression GnRH‐I. Female mice (n = 6) were vaccinated at days 1 30 with recombinant antigen for immunocastration induced preferentially Th1/Th2, Th2,...