A5044206394- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Sperm and Testicular Function
- Estrogen and related hormone effects
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Proteoglycans and glycosaminoglycans research
- Glutathione Transferases and Polymorphisms
- Hormonal and reproductive studies
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Sirtuins and Resveratrol in Medicine
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Hypothalamic control of reproductive hormones
- Glycosylation and Glycoproteins Research
- FOXO transcription factor regulation
- Mechanisms of cancer metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Chemical Reactions and Isotopes
- Bone and Dental Protein Studies
- Bone health and treatments
University of Chile
2013-2024
Pontificia Universidad Católica de Chile
2024
Clínica Las Condes
2000
The University of Texas at Austin
1989
// Catherine A. Sánchez 1 , Eliana I. Andahur 1,2 Rodrigo Valenzuela 3 Enrique Castellón Juan Fullá Christian G. Ramos and C. Triviño 4 Urology Department, Las Condes Clinic, Santiago, Chile 2 Faculty of Science, University Chile, Medicine, Sistemas Genómicos, Valencia, España Correspondence to: Sánchez, email: Keywords : prostate cancer, miRNAs, exosomes, next generation sequencing, niche Received June 17, 2015 Accepted November 25, Published...
Resveratrol is a polyphenol found at high concentrations in grapes and red wine with reported anticarcinogenic effects. We studied the molecular mechanism of resveratrol-induced apoptosis proliferation arrest prostate derived cells PZ-HPV-7 (nontumorigenic line), LNCaP (androgen-sensitive cancer PC-3 (androgen-insensitive line). Apoptosis cell cycle distribution were evaluated by flow cytometry MTT assay direct counting. Caspases, bax, bcl-2, cyclins, Cdks, p53, p21, p27 measured Western...
An increase in circulating platelets, or thrombocytosis, is recognized as an independent risk factor of bad prognosis and metastasis patients with ovarian cancer; however the complex role platelets tumor progression has not been fully elucidated. Platelet activation associated epithelial to mesenchymal transition (EMT), while Tissue Factor (TF) protein expression by cancer cells shown correlate hypercoagulable state metastasis. The aim this work was determine effect platelet-cancer cell...
// Sumie Kato 1 , Lorena Abarzua-Catalan César Trigo Ana Delpiano Cristobal Sanhueza 2 Karen García Carolina Ibañez Katherine Hormazábal Daniela Diaz Jorge Brañes Enrique Castellón 4 Erasmo Bravo 5 Gareth Owen 3 and Mauricio A. Cuello Division of Obstetrics Gynecology, Faculty Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile Department Hematology Oncology, Physiological Sciences, Biological University Gynecologic Oncology...
In the early stages, prostate cancer is androgen‑ dependent; therefore, medical castration has shown significant results during initial stages of this pathology. Despite effect, advanced resilient to such treatment. Recent evidence shows that derivatives Cannabis sativa and its analogs may exert a protective effect against different types oncologic pathologies. The purpose present study was detect presence cannabinoid receptors (CB1 CB2) on cells with prostatic origin evaluate in vitro use...
Abstract BACKGROUND Multidrug resistance (MDR) proteins have been associated with the lack of chemotherapy response. Expression these has described in prostate, but there is no information about their role response prostate cancer (PC). We studied gene and protein expression MDR primary cell cultures from PC tumors lines, relationship effects on survival. METHODS Primary were obtained samples provided by our Institutional Hospital. Cell lines LNCaP, PC3, DU145 also examined. Cells treated...
Polyphenols have been proposed as antitumoral agents. We shown that resveratrol (RES) induced cell cycle arrest and promoted apoptosis in prostate cancer cells by inhibition of the PI3K pathway. The RES effects on NF kappaB activity LNCaP (inducible kappaB), PC-3 (constitutive kappaB) are reported.Cells were treated with 1-150 microM during 36 hr. subcellular localization was analyzed western blot immunofluorescence. I alpha evaluated immunoprecipitation followed Western blot. Specific DNA...
Recently, we produced a tumour antigen-presenting cells (TAPCells) vaccine using melanoma cell lysate, called TRIMEL, as an antigen source and activation factor. Tumour induced immunological responses increased patient survival. Herein, investigated the effect of TAPCells loaded with prostate cancer lysates (PCCL) source, TRIMEL dendritic (DC) factor; which were co-injected Concholepas concholepas haemocyanin (CCH) adjuvant on castration-resistant (CRPC) patients. The lysate mix capacity,...
Cancer stem cells (CSCs) have the ability to self-renew and differentiate give rise heterogeneous phenotype of tumor cells. It is believed that these are involved in metastasis, recurrence therapy resistance various cancers. CSCs been identified prostate cancer (PCa), one most diagnosed malignancies men over world, for which chemotherapy a major problem treatment castration-resistant advanced stages. Molecular signatures, gene expression functional features reported PCa CSCs. Most data come...
The incidence and mortality rates of prostate cancer (PCa) are increasing, PCa is almost the second‑leading cause cancer‑associated in men. During tumor progression, epithelial cells decrease number adhesion molecules, change their polarity position, rearrange cytoskeleton increase migratory invasive capacities. These changes known under concept epithelial‑mesenchymal transition (EMT). EMT characterized by an upregulation certain transcription factors, including SNAIL1, which represses genes...
Secreted protein acidic and rich in cysteine (SPARC), or osteonectin, is a matricellular that modulates interactions between cells their microenvironment. SPARC expressed during extracellular matrix remodeling abundant bone marrow high-grade prostate cancer (PCa). In PCa, induces changes associated with epithelial-mesenchymal transition (EMT), enhancing migration invasion increasing the expression of EMT transcriptional factor Zinc finger E-box-binding homeobox 1 (ZEB1), but not SNAI1...
Prostate cancer represents a major concern in human oncology and the phytoalexin resveratrol (RES) inhibits growth proliferation of prostate cells through induction apoptosis. In addition, previous data indicate that oestrogen-responsive breast cells, RES induces apoptosis by inhibition phosphoinositide-3-kinase (PI3K) pathway. Here, using androgen receptor (AR)-positive LNCaP oestrogen alpha (ERα)-expressing PC-3 tumour we have analysed whether antiproliferative activity takes place AR- or...
Prostate cancer (PC) is a leading male oncologic malignancy wideworld. During malignant transformation, normal epithelial cells undergo genetic and morphological changes known as epithelial-mesenchymal transition (EMT). Several regulatory genes specific marker proteins are involved in PC EMT. Recently, syndecans have been associated with grade Gleason score PC. Considering that SNAIL mainly gene repressor increased syndecan promoters putative binding sites for this repressor, we propose...
Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide.Chemotherapy response very poor and resistance to hormone-based treatments frequent advances stages.Recently, tumor-initiating cells or stem (CSCs) have been identifi ed several cancers, including PCa.These are thought be responsible for therapy resistance, relapse metastasis.In present work, enriched populations of CSCs were obtained using a mixed procedure that included diff erential clone-forming ability,...
Abstract BACKGROUND In several cancer types, expression of multidrug resistance (MDR) proteins has been associated with lack chemotherapy response. advanced prostate (PCa) the use is mainly palliative due to its high resistance. Previously, we described that MDR phenotype in PCa could be related basal and drug‐induced P‐Glycoprotein (P‐Gp), MRP1, LRP. METHODS Using primary cell cultures from patients, evaluated effect function inhibition P‐Gp, LRP, on survival after exposure. Cells were...
It has been reported that one of the factors promotes tumoral progression is abnormal activation epithelial–mesenchymal transition program. This process associated with cells acquiring invasive and malignant properties transcription factor zinc finger E-box-binding homeobox 1 (ZEB1) as its main activators. However, role ZEB1 in promoting malignancy prostate cancer (PCa) still unclear. Here, we report expression correlates Gleason score PCa samples regulates characteristics cell lines. The...
Metastatic prostate cancer (mPCa) is one of the most prevalent cancers in men worldwide. The main cause death these patients androgen-resistant metastatic disease. Surgery primary tumor has been avoided as there no strong evidence that supports a beneficial effect. From biological point view, it appears rational to hypothesize may contribute establishment and growth metastases. Considering this, we propose cytoreductive surgery (CS) advanced stage slows progression To test used mouse model...
One of the factors promoting tumoral progress is abnormal activation epithelial–mesenchymal transition (EMT) program which has been associated with chemoresistance in cells. The transcription factor zinc finger E-box-binding homeobox 1 (ZEB1), a key EMT activator, recently related to docetaxel resistance, main chemotherapeutic used advanced prostate cancer treatment. mechanisms involved this protective effect are still unclear. In previous work, we demonstrated that ZEB1 expression induced...
Abstract Cancer stem cells (CSCs) are a small subpopulation of quiescent with the potential to differentiate into tumor cells. CSCs involved in initiation and progression contribute treatment failure through their intrinsic resistance chemo- or radiotherapy, thus representing substantial concern for cancer treatment. Prostate CSCs’ activity has been shown be regulated by transcription factor Signal Transducer Activator Transcription 3 (STAT3). Here we investigated effect galiellalactone...
Contradictory data have been reported regarding the effect of GnRH agonists and antagonists on cell growth survival, using prostate cancer-derived lines expressing either endogenous or exogenous receptors. We addressed issue studying leuprolide (agonist) cetrorelix (antagonist) growth, apoptosis receptor expression a primary coculture system. Also, binding characteristics in this culture system are described. Epithelial stromal cells were obtained from adenocarcinoma samples cocultured...