A5038426102- Neurobiology and Insect Physiology Research
- Neuroblastoma Research and Treatments
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Cancer therapeutics and mechanisms
- Zebrafish Biomedical Research Applications
- Signaling Pathways in Disease
- Hepatitis B Virus Studies
- Oral Health Pathology and Treatment
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- Developmental Biology and Gene Regulation
- Prostate Cancer Treatment and Research
- Axon Guidance and Neuronal Signaling
- Circadian rhythm and melatonin
- RNA regulation and disease
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Physiological and biochemical adaptations
- Vitamin D Research Studies
- Radiology practices and education
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Insect symbiosis and bacterial influences
Institute for Biomedicine
2024
University of Gothenburg
2015-2024
American University of Beirut
2023-2024
Stockholm University
2023-2024
National Centre for Biological Sciences
2023-2024
Tata Institute of Fundamental Research
2023-2024
Tata Institute for Genetics and Society
2023-2024
AstraZeneca (Sweden)
2021-2022
Umeå University
2017-2018
Mexican Social Security Institute
2005-2013
Aberrant activation of anaplastic lymphoma kinase (ALK) has been described in a range human cancers, including non-small cell lung cancer and neuroblastoma (Hallberg Palmer, 2013). Vertebrate ALK considered to be an orphan receptor the identity ligand(s) is critical issue. Here we show that FAM150A FAM150B are potent ligands for bind extracellular domain addition wild-type able drive 'superactivation' activated mutants from neuroblastoma. In conclusion, our data robustly by FAM150A/B provide...
Significance Neuroblastoma is a pediatric tumor arising from the neural crest. Dysregulation of receptor tyrosine kinase ALK has been linked to neuroblastoma, making it important understand its function in native conditions. In zebrafish, related receptor—Ltk—is also expressed crest and regulates development specific pigment cells—iridophores. Ligands activating human were recently identified as ALKAL proteins (FAM150, AUG) by biochemical means. Our data show that this ligand–receptor pair...
High-risk neuroblastoma (NB) is responsible for a disproportionate number of childhood deaths due to cancer.One indicator highrisk NB amplification the neural MYC (MYCN) oncogene, which currently therapeutically intractable.Identification anaplastic lymphoma kinase (ALK) as an oncogene raised possibility using ALK tyrosine inhibitors (TKIs) in treatment patients with activating mutations.8-10% primary are ALK-positive, figure that increases relapsed population.ALK activated by ALKAL2 ligand...
Abstract Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install genomic insertions using an Sp Cas9 nuclease-based prime editor (PEn). We first demonstrate PEn coupled regular guide RNA (pegRNA) efficiently promotes short through homology-dependent DSB mechanism. While leads increased levels of by-products, it can rescue pegRNAs perform poorly with nickase-based...
Abstract Background Cervical carcinoma (CC) is one of the most common cancers among women worldwide and first cause death Mexican female population. CC progression shows a continuum neoplastic transitions until invasion. Matrix metalloproteinases (MMPs) cathepsins play central role on enhancement tumor-induced angiogenesis, cell migration, proliferation, apoptosis connective tissue degradation. MMPs -2 -9 expression has been widely studied in cervical cancer. Nevertheless, no other or have...
Abstract BACKGROUND Multidrug resistance (MDR) proteins have been associated with the lack of chemotherapy response. Expression these has described in prostate, but there is no information about their role response prostate cancer (PC). We studied gene and protein expression MDR primary cell cultures from PC tumors lines, relationship effects on survival. METHODS Primary were obtained samples provided by our Institutional Hospital. Cell lines LNCaP, PC3, DU145 also examined. Cells treated...
Abstract BACKGROUND In several cancer types, expression of multidrug resistance (MDR) proteins has been associated with lack chemotherapy response. advanced prostate (PCa) the use is mainly palliative due to its high resistance. Previously, we described that MDR phenotype in PCa could be related basal and drug‐induced P‐Glycoprotein (P‐Gp), MRP1, LRP. METHODS Using primary cell cultures from patients, evaluated effect function inhibition P‐Gp, LRP, on survival after exposure. Cells were...
The Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) plays a critical role in the specification of founder cells (FCs) Drosophila visceral mesoderm (VM) during embryogenesis. Reporter gene and CRISPR/Cas9 deletion analysis reveals enhancer regions upstream Alk locus that influence tissue-specific expression amnioserosa (AS), VM epidermis. By performing high throughput yeast one-hybrid screens (Y1H) with library transcription factors (TFs) we identify Odd-paired (Opa),...
Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila , including functions central nervous system (CNS), however molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of signaling larval CNS. TaDa was CNS tissues, while genetically manipulating output. The resulting data were analysed together with scRNA-seq datasets performed under similar conditions, identifying...
Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila , including functions central nervous system (CNS), however molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of signaling larval CNS. TaDa was CNS tissues, while genetically manipulating output. The resulting data were analyzed together with scRNA-seq datasets performed under similar conditions, identifying...
ABSTRACT Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in circular mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify targets embryos overexpressing Jeb versus with abrogated activity, revealing differentially expressed genes, including...
ABSTRACT Activating anaplastic lymphoma kinase (ALK) receptor tyrosine (RTK) mutations occur in pediatric neuroblastoma and are associated with poor prognosis. To study ALK-activating a genetically controllable system, we employed CRIPSR/Cas9, incorporating orthologs of the human oncogenic ALKF1174L ALKY1278S Drosophila Alk locus. AlkF1251L AlkY1355S mutant exhibited enhanced signaling phenotypes, but unexpectedly depended on Jelly belly (Jeb) ligand for activation. Both larval brains...
Abstract Anaplastic lymphoma kinase (Alk) is a receptor tyrosine of the insulin super-family that functions as oncogenic driver in range human cancers such neuroblastoma. In order to investigate mechanisms underlying Alk signaling, we conducted genetic suppressor screen Drosophila melanogaster . Our identified multiple loci important for including members Ras/Raf/ERK-, Pi3K-, and STAT-pathways well tailless (tll) foxo whose orthologues NR2E1/TLX FOXO3 are transcription factors implicated...
Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila , including functions central nervous system (CNS), however molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of signaling larval CNS. TaDa was CNS tissues, while genetically manipulating output. The resulting data were analysed together with scRNA-seq datasets performed under similar conditions, identifying...
Summary 7‐alpha‐Methyl‐19‐Nortestosterone (MENT) is a synthetic androgen more potent than testosterone (T) and cannot be reduced at 5‐alpha position. No important effects of MENT on prostate growth have been reported. However, little known about the effect benign prostatic hyperplasia (BPH) or carcinoma (CaP). We evaluate MENT, T dihydrotestosterone (DHT) secretion, proliferation gene expression primary cell cultures from human BPH CaP. Moreover, these androgens was examined in presence...