A5023714363- Research in Cotton Cultivation
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Silkworms and Sericulture Research
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Polymer-Based Agricultural Enhancements
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Irrigation Practices and Water Management
- Plant Stress Responses and Tolerance
- Innovations in Aquaponics and Hydroponics Systems
- Biofuel production and bioconversion
- Rice Cultivation and Yield Improvement
ZheJiang Academy of Agricultural Sciences
2023-2024
Anhui Institute of Robotics Industrial Technology Research Institute
2024
Nanjing Agricultural University
2014-2023
Zhejiang University
2020-2023
Cotton Research Institute
2014
Interspecific hybridization and whole genome duplication are driving forces of genomic organism diversification. But the effect interspecific on non-coding portion in particular remains largely unknown. In this study, we examine profile long RNAs (lncRNAs), comparing them with that coding genes allotetraploid cotton (Gossypium hirsutum), its putative diploid ancestors (G. arboreum; G. raimondii), an F1 hybrid arboreum × raimondii, AD).We find most lncRNAs (80%) were allelic expressed genome....
Gossypium australe F. Mueller (2n = 2x 26, G2 genome) possesses valuable characteristics. For example, the delayed gland morphogenesis trait causes cottonseed protein and oil to be edible while retaining resistance biotic stress. However, lack of gene sequences their alternative splicing (AS) in G. remain unclear, hindering explore species-specific biological morphogenesis.Here, we report first sequencing full-length transcriptome Australian wild cotton species, australe, using Pacific...
In 2019, Kaya-Okur et al. reported on the cleavage under targets and tagmentation (CUT&Tag) technology for efficient profiling of epigenetically modified DNA fragments. It was used mainly cultured cell lines especially effective small samples single cells. This strategy generated high-resolution low-background-noise chromatin data epigenomic analysis. CUT&Tag is well suited to be in plant cells, tissues from which are taken, such as ovules, anthers, fibers.Here, we present a protocol step by...
Abstract The genomic shock of whole-genome duplication (WGD) and hybridization introduces great variation into transcriptomes, for both coding noncoding genes. An altered transcriptome provides a molecular basis improving adaptation during the evolution new species. allotetraploid cotton, together with putative diploid ancestor species compose fine model study rapid gene neofunctionalization over genome shock. Here we report on Drought-Associated Non-coding 1 (DAN1), long intergenic RNA...
Replacement of polyethylene (PE) film mulch (PM) to biodegradable (BM) can significantly reduce plastic pollution and carbon footprint in agricultural production. However, the effects BM on nitrogen dynamics soil-plant system remain elusive. A field experiment with two cropping seasons was conducted investigate soil mineralization, nitrate accumulation leaching, plant uptake. Although distribution water under treatment similar that PM at early growth stage potato plants, decomposition during...
Verticillium dahliae, one of the most destructive fungal pathogens several crops, challenges sustainability cotton productivity worldwide because very few widely-cultivated Upland varieties are resistant to wilt (VW). Here, we report that REVEILLE2 (RVE2), Myb-like transcription factor, confers novel function in resistance VW by regulating jasmonic acid (JA) pathway cotton. RVE2 expression was essentially required for activation JA-mediated disease-resistance response. physically interacted...
Numerous endogenous and environmental signals regulate the intricate highly orchestrated process of plant senescence. Ethylene (ET), which accumulates as senescence progresses, is a major promoter leaf The master transcription activator ETHYLENE INSENSITIVE3 (EIN3) activates expression wide range downstream genes during Here, we found that unique EIN3-LIKE 1 (EIL1) gene, cotton LINT YIELD INCREASING (GhLYI), encodes truncated EIN3 protein in upland (Gossypium hirsutum L.) functions an ET...
Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluable characteristics unavailable in the tetraploid gene pool, such as resistance pests and diseases tolerance abiotic stresses. However, it is quite difficult transfer favorable traits into Upland through conventional methods due cross-incompatibility of G. hirsutum 52, AADD) arboreum. Here, we improved an embryo rescue technique overcome between these two parents for transferring genes from...
Natural antisense transcripts (NATs) in model plants have been recognized as important regulators of gene expression under abiotic stresses. However, the functional roles NATs crops low temperature are still unclear. Here, we identified 815 and 689 from leaves Gossypium hirsutum G. barbadense chilling stress. Among those, 224 were interspecific homologs between two species. The correlation coefficients for their cognate sense genes (CSG) 0.43 0.37 barbadense, respectively. Furthermore, CSGs...
In contrast to CUT&Tag approaches for profiling bulk histone modifications, current methods analysing specific transcription factor (TF)-DNA interactions remain technically challenging due TFs having relatively low abundance. Moreover, an efficient strategy plant is not yet available. Here, we first applied biotinylated Tn5 transposase-mediated (B-CUT&Tag) produce high-quality libraries interrogating TF-DNA interactions. B-CUT&Tag combines streptavidin-biotin-based DNA purification with...
Gossypium anomalum (BB genome) possesses the desirable characteristics of drought tolerance, resistance to diseases and insect pests, potential for high quality fibers. However, it is difficult transfer genes associated with these traits into cultivated cotton (G. hirsutum, AADD genome). Monosomic alien addition lines (MAALs) can be used as a bridge desired from wild species G. hirsutum. In cotton, however, number smaller size chromosomes has resulted in difficulties discriminating...
Cotton production in Xinjiang is crucial to China's economy, but the region's cold climate poses challenges cultivation. This study analyzes temperature data from 33 meteorological stations (1981–2020) alongside cotton yield assess damage during key growth stages. A comparison made with cotton-producing counties U.S. southwest (Texas, Kansas, Oklahoma). Results show that has a shorter frost-free period (140–210 days) compared (235–300 days). The Pearson correlation coefficient indicates...
We previously reported the development of a set Gossypium hirsutum-G. australe alien chromosome addition lines. Naturally, however, G. exchanges were very limited, impeding stable transference useful genes from (G2G2 genome) into most cultivated cotton, hirsutum (AADD). In present report, pollen pentaploid (2n = AADDG2) was irradiated with seven different doses ranging 10 to 40 Grays and used pollinate emasculated flowers over three consecutive years. Irradiation greatly increased genetic...
Sugar metabolism influences the quality of sweet corn (Zea mays var. saccharate Sturt) kernels, which is a major goal for maize breeding. In this study, genome-wide transcriptomes from two supersweet cultivars (cv. Xuetian 7401 and Zhetian 11) with nearly two-fold difference in kernel sugar content were carried out to explore genes related metabolism. total, 45,748 differentially expressed (DEGs) kernels 596 DEGs leaves identified. PsbS, photosynthetic system II subunit S, showed isoforms...
The noncoding regions throughout the genome are in large part comprised of transposable elements (TEs), some which functionalized with long intergenic RNAs (lincRNAs). DNA methylation is predominantly associated TEs, but little known about its contribution to transcription lincRNAs. Here, we examine lincRNA profiles methylation-related mutants five species, Arabidopsis, rice, tomato, maize, and mouse, elucidate patterns regulation under altered status. Significant activation lincRNAs was...
Abstract Polyploidization, or whole‐genome duplication, leads to gene expression changes that drive the evolution of many plants and some animals. However, role dosage effect on following genome duplication remains unclear. Here, several Gossypium hirsutum‐Gossypium australe alien chromosome addition lines (ALs) were developed contained a single (MA) pair (DA) G. chromosomes hirsutum background, which can be regarded as mimic elucidate short‐term effects changes. Therefore, profile was...
Abstract Strand-specific RNA-seq is a powerful tool for the discovery of novel transcripts, annotation genomes, and profiling gene expression levels. Tn5 transposase has been successfully applied in massive-scale sequencing projects; particular, adaptor modification used epigenetics, genomic structure, chromatin visualization. We developed dU-adaptor-assembled Tn5-mediated strand-specific RNA-sequencing protocol compared this method with leading dUTP terms experimental procedure multiple...