A5000284231- Virus-based gene therapy research
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Gut microbiota and health
- RNA modifications and cancer
- interferon and immune responses
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Biochemical effects in animals
- Hepatitis C virus research
- Vector-borne infectious diseases
- T-cell and Retrovirus Studies
- Hepatitis B Virus Studies
- Vibrio bacteria research studies
- Respiratory viral infections research
- Genetics and Physical Performance
- CRISPR and Genetic Engineering
- Sulfur Compounds in Biology
- Poxvirus research and outbreaks
- Liver physiology and pathology
- Animal Genetics and Reproduction
MRC University of Glasgow Centre for Virus Research
2021-2024
The Pirbright Institute
2019-2021
International Centre for Genetic Engineering and Biotechnology
2017-2019
All India Institute of Medical Sciences
2014-2017
National Brain Research Centre
2015
Boston Children's Hospital
1996
Abstract The innate response to a pathogen is critical in determining the outcome of infection. However, interplay different cellular responses that are activated following viral infection and their contribution antiviral signalling has not been clearly established. This work shows flaviviruses, including Dengue, Zika, West Nile Tick-borne encephalitis viruses, activate unfolded protein before transcription interferon regulatory factor 3 induced genes. Infection conditions priming leads...
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was decline until 2016, when massive outbreak affected country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya dengue viruses along with clinical comorbidity features. We recruited patients during evaluated them virus antibodies RNA through IgM, NS1 antigen quantitative real-time PCR (qPCR). further Zika by qPCR. Additionally,...
A commensal microbiota regulates and is in turn regulated by viruses during host infection which can influence virus infectivity. In this study, analysis of colon population changes following a low pathogenicity avian influenza (AIV) the H9N2 subtype two different chicken breeds was conducted. Colon samples were taken from control infected groups at various timepoints post infection. 16S rRNA sequencing on an Illumina MiSeq platform performed data mapped to operational taxonomic units...
A specific targeting modality for hepatocellular carcinoma (HCC) could ideally encompass a liver cell delivery system of transcriptional unit that is active only in neoplastic cells. Sendai virosomes, derived from viral envelopes, home to hepatocytes based on the expression asialoglycoprotein receptors (ASGPRs) which are recognized by virosomal fusion (F) proteins. As reported earlier us and other groups, gene silencing (TGS) does not require continuous presence effector siRNA/shRNA molecule...
Phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) regulation of the GnRH gene was studied in two mouse neuronal cell lines, Gn11 and NLT. TPA treatment NLT cells grown low serum conditions did not alter endogenous mRNA levels, indicating that is regulated by phorbol esters under these conditions. This result confirmed transfection studies which change expression a GnRH-luciferase reporter construct. In contrast, stimulated human construct, correlating with protoon-cogenes c-fos...
Abstract Cross-species transmission of avian influenza A viruses (IAVs) into humans could represent the first step a future pandemic 1 . Multiple factors limiting spillover and adaptation IAVs in have been identified, but they are not sufficient to explain which virus lineages more likely cross species barrier 1,2 Here, we identified human BTN3A3 3 (butyrophilin subfamily member A3) as potent inhibitor IAVs. We determined that is constitutively expressed airways its antiviral activity...
Liver is a key organ involved in the regulation of both systemic as well local inflammatory responses. Hepatic inflammation hallmark viral hepatitis caused by non-cytopathic Hepatitis B and C viruses (HBV HCV). Both HBV HCV induce several responses, causing persistent liver injury, which manifests into progressive diseased state. This ultimately leads to fibrosis, cirrhosis eventually hepatocellular carcinoma. The disease progression mediated complex interplay molecular pathways involving...
Placental like alkaline phosphate (PLAP), an oncofetal antigen, is highly expressed in germ cell, cervical, ovarian and several other tumour types but minimally normal tissues. The expression of a PLAP promoter based transcriptional unit following antigen mediated cell specific delivery possible approach for targeting. alone or combination with NFκB DNA response elements was used expressing shRNA targeting the long control region (LCR) human papillomavirus (HPV)-16 oncogenes E6 E7 via gene...
H5N1 avian influenza virus (lineage 2.3.4.4b, B3.13 genotype) has caused, unexpectedly, a large outbreak in dairy cattle North America. It is critical to ascertain how this specifically adapted bovine cells and the molecular determinants of process. Here, we focused on contribution viral internal genomic segments adaptation. We generated 45 reassortant viruses harbouring haemagglutinin neuraminidase from A/Puerto Rico/8/1934 gene constellations several A (IAV) or carrying segment swaps...
Objective c‐Myc is one of the central players oncogenesis in hepatocellular carcinoma (HCC) and also required normal development. Majority transcripts are transcribed by its P2 promoter an upstream ME1a1 site transcriptional activation. dsRNA recruits epigenetic silencing complexes inducing long term/heritable repression. We devised a novel HCC targeting system studied mechanism. Methods Firstly, alpha‐fetoprotein (AFP) promoter/enhancer was used to express shRNA against site. Secondly, we...
Various influenza A viruses (AIVs) have infected humans in the last decade. These infections result from spill over into at times of AIV poultry outbreaks with severe clinical consequences for those infected. AIVs do not ability to be efficiently spread among via respiratory droplets or aerosols and subsequently cause a pandemic. However, adaptation these avian by mutation reassortment may change this. We know that require several critical characteristics order effectively between humans....
Abstract A commensal microbiome regulates and is in turn regulated by viruses during host infection which can influence virus infectivity. In this study, analysis of colon population changes following a low pathogenicity avian influenza (AIV) the H9N2 subtype two different chicken breeds was conducted. Using 16S rRNA sequencing subsequent data we found reduced alpha diversity acute period AIV (day 2-3) both Rhode Island Red VALO lines. From day 4 post gradual increase observed, but did not...