A5033720124- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Inflammasome and immune disorders
- Complement system in diseases
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Electron and X-Ray Spectroscopy Techniques
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Bacterial biofilms and quorum sensing
- Heme Oxygenase-1 and Carbon Monoxide
- Characterization and Applications of Magnetic Nanoparticles
- Lipid Membrane Structure and Behavior
- Porphyrin and Phthalocyanine Chemistry
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- Viral Infections and Immunology Research
- Electrochemical sensors and biosensors
- Blood groups and transfusion
- RNA Research and Splicing
- Respiratory viral infections research
Charité - Universitätsmedizin Berlin
2024
Humboldt-Universität zu Berlin
2024
Freie Universität Berlin
2024
Max Delbrück Center
2024
Leiden University
2016-2021
Advanced Research Center for Nanolithography (Netherlands)
2019
Utrecht University
2012-2015
Leiden University Medical Center
2012-2015
Hexing Complement activation is an immediate and potent immune defense mechanism, but how immunoglobulin G (IgG) antibodies activate complement at the molecular level poorly understood. Using high-resolution crystallography, Diebolder et al. (p. 1260 ) show that human IgGs form hexameric structures by interacting with neighboring IgG molecules, complex then activates complement. Thus, molecules system can coexist in blood because will only be triggered after senses a surface antigen starts...
Antigen binding by serum Ig-M (IgM) protects against microbial infections and helps to prevent autoimmunity, but causes life-threatening diseases when mistargeted. How antigen-bound IgM activates complement-immune responses remains unclear. We present cryoelectron tomography structures of IgM, C1, C4b complexes formed on antigen-bearing lipid membranes normal human at 4 °C. The IgM-C1-C4b revealed product release as the temperature-limiting step in complement activation. Both hexamers...
The NOD-like receptors NAIP5 and NLRC4 play an essential role in the innate immune response to bacterial tail protein flagellin. Upon flagellin detection, form a hetero-oligomeric inflammasome that induces caspase-1-dependent cell death. So far, both mechanism of formation NAIP5-NLRC4 its structure are poorly understood. In this study we combine reconstitution HEK293 cells, purification components, negative stain electron microscopy address these issues. We find Salmonella typhimurium...
Structural studies of translating ribosomes traditionally rely on in vitro assembly and stalling defined states. To comprehensively visualize bacterial translation, we reactivated ex vivo-derived E. coli polysomes the PURE translation system analyzed actively elongating by cryo-EM. We find that 31% 70S assemble into disome complexes represent eight distinct functional states including decoding termination intermediates, a pre-nucleophilic attack state. The diversity together with RNase...
Orthomyxoviruses, such as influenza and thogotoviruses, are important human animal pathogens. Their segmented viral RNA genomes wrapped by nucleoproteins (NPs) into helical ribonucleoprotein complexes (RNPs). NP structures of several viruses have been reported. However, there still contradictory models how orthomyxovirus RNPs assembled. Here, we characterize the crystal structure Thogoto virus (THOV) found striking similarities to NPs, including a two-lobed domain architecture, positively...
During neurotransmission, presynaptic action potentials trigger synaptic vesicle fusion with the plasma membrane within milliseconds. To visualize dynamics before, during, and right after at central synapses under near-native conditions, we developed an experimental strategy for time-resolved in situ cryo-electron tomography millisecond temporal resolution. We coupled optogenetic stimulation cryofixation confirmed stimulation-induced release of neurotransmitters via cryo-confocal microscopy...
In Escherichia coli , membrane-bound chemoreceptors, the histidine kinase CheA, and coupling protein CheW form highly ordered chemosensory arrays. core signaling complexes, chemoreceptor trimers of dimers undergo conformational changes, induced by ligand binding sensory adaptation, which regulate activation. Here, we characterize cryo-electron tomography kinase-ON kinase-OFF conformations E. serine receptor in its native array context. We found distinctive structural differences between...
Abstract The bacterial cell wall is a multicomponent structure that provides structural support and protection. In monoderm species, the made up predominantly of peptidoglycan, teichoic acids capsular glycans. Filamentous Actinobacteria incorporate new cell-wall material at their tips. Here we use cryo-electron tomography to reveal architecture actinobacterial Streptomyces coelicolor . Our data shows density difference between apex subapical regions. Removal results in patchy distinct...
Chemical modifications of ribosomal RNAs (rRNAs) and proteins expand their topological repertoire, together with the plethora bound ligands, fine-tune function. Detailed knowledge this natural composition provides important insights into ribosome genesis function clarifies some aspects ribosomopathies. The discovery new structural properties functional ribosomes has gone hand in cryo-electron microscopy (cryo-EM) its technological development. In line ability to visualize atomic details - a...
Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm possess unusual polygonal cell shape with sharp ridges that run along the body. Previously, a putative surface protein layer (S-layer) was observed as outermost of these bacteria. We hypothesized this S-layer is determining factor for their shape. Therefore, we enriched from M. lanthanidiphila cells through LC-MS/MS...
Abstract The CsmA protein from the baseplate of Chlorobaculum tepidum is proposed as an attractive motif for engineering semisynthetic materials artificial photosynthesis. We perform self‐assembly zinc protoporphyrin IX (ZnPP) with a truncated (tCsmA) amphipathic peptide scaffold bottom‐up construction matrix energy transfer. resulting shows extended periodic structure 7 2 helical symmetry. complex able to maintain good photostability at least 30 min when concentration more than 20 μM, and...
Abstract The bacterial cell wall is a dynamic, multicomponent structure that provides structural support for shape and physical protection from the environment. In monoderm species, thick made up predominantly of peptidoglycan, teichoic acids variety capsular glycans. Filamentous Actinobacteria, such as Streptomyces coelicolor , incorporate new material at apex their hyphal cells during growth. this study we use cryo-electron tomography to reveal architecture bacterium. Our data shows...