A5000799051- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Ion-surface interactions and analysis
- Molecular Junctions and Nanostructures
- Nanofabrication and Lithography Techniques
- Analytical chemistry methods development
- Mechanical and Optical Resonators
- 3D Printing in Biomedical Research
- biodegradable polymer synthesis and properties
- Advanced Drug Delivery Systems
- Drug Solubulity and Delivery Systems
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Bacterial biofilms and quorum sensing
- Near-Field Optical Microscopy
- Bone Tissue Engineering Materials
- Advanced Polymer Synthesis and Characterization
- Integrated Circuits and Semiconductor Failure Analysis
- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Electron and X-Ray Spectroscopy Techniques
- DNA and Nucleic Acid Chemistry
University of Nottingham
2010-2023
University of Sheffield
2005-2023
Surface (Brazil)
2010
Steklov Mathematical Institute
2010
National Physical Laboratory
2009
University of Antwerp
2000-2007
Ghent University
2007
Massachusetts Institute of Technology
1999-2007
University of Portsmouth
2006
Philipps University of Marburg
2002-2005
For two series of polyethylenimine-graft-poly(ethylene glycol) (PEI-g-PEG) block copolymers, the influence copolymer structure on DNA complexation was investigated and physicochemical properties these complexes were compared with results blood compatibility, cytotoxicity, transfection activity assays. In first series, PEI (25 kDa) grafted to different degrees substitution PEG (5 in second molecular weight (MW) varied (550 Da 20 kDa). Using atomic force microscopy, we found that strongly...
The scientific community needs a rapid and reliable way of accurately determining the stiffness atomic-force microscopy cantilevers. We have compared experimentally determined values for ten cantilever probes using four different methods. For rectangular silicon beams well defined geometry, approaches all yield within 17% manufacturer's nominal stiffness. One methods is new, based on acquisition analysis thermal distribution functions oscillator's amplitude fluctuations. evaluate this method...
The attachment of biomolecules, in particular proteins, onto solid supports is fundamental the development advanced biosensors, bioreactors, affinity chromatographic separation materials, and many diagnostic techniques. In addition, effective investigation biomolecular structure function with scanning probe microscopy often requires a strong biomolecule to substrate. Here, we investigate binding protein catalase gold surfaces modified by self-assembled monolayers (SAMs). chemical physical...
An atomic force microscope (AFM) has been used to directly monitor specific interactions between antibodies and antigens employed in an immunoassay system. Results were achieved using AFM probes functionalized with ferritin, monitoring the adhesive forces probe anti-ferritin antibody-coated substrates. Analysis of distribution data suggests a quantization forces, period 49 ± 10 pN. This periodic may be attributed single unbinding events individual antigen antibody molecules. These results...
Copolymers of polylactide and poly(ethylene glycol) (PLA−PEG), which self-disperse in water to form spherical nonionic micelles, have been investigated as a novel biodegradable drug delivery system. These copolymers are defined by the molecular weight ratios their components (1.5:2 PLA−PEG 2:5 PLA−PEG) gave two peaks when purified gel permeation chromatography (GPC). The first peak consisted micelles with diameter 15.6 nm for 1.5:2 PLA−PEG, 18.9 after analysis dynamic light scattering (DLS)...
Controlling receptor-mediated interactions between cells and template surfaces is a central principle in many tissue engineering procedures (1-3). Biomaterial engineered to present cell adhesion ligands undergo integrin-mediated molecular with (1, 4, 5), stimulating spreading, differentiation (6-8). This provides mechanism for mimicking natural cell-to-matrix interactions. Further sophistication the control of can be achieved by fabricating on which spatial distribution restricted...
Abstract The colonization of biodegradable polymer scaffolds with cell populations has been established as the foundation for engineering a number tissues, including cartilage, liver, and bone. Within these scaffolds, cells encounter porous environment in which they must migrate across convoluted surface to generate homogenous distribution. Predicting interactions between pores is important if scaffold characteristics are be optimized. Therefore, we investigated behavior two model types over...
We report the use of supercritical carbon dioxide (scCO2) to create a diverse range polymeric composites incorporating thermal and solvent labile guest materials such as proteins; no additional co-solvents are required; entire process can be carried out at near ambient conditions; polymer morphology is controllable; high loadings species achieved protein function retained.
1. Materials containing hyaluronic acid have been prepared by filtration (Ogston & Stanier, 1950) from ox synovial fluid and a protein-rich human mesothelioma fluid. The material has deproteinized treatment with chloroform pentanol gradient elution on DEAE-Sephadex; several fractions were obtained the latter method. These materials can be stored in solution at -20 degrees without change of properties. contained 21% protein; all other preparations less than 6% protein. 2. two compared...
The current gold standard for the culture of human pluripotent stem cells requires use a feeder layer cells. Here, we develop spatially defined system based on UV/ozone radiation modification typical cell plastics to define favorable surface environment culture. Chemical and geometrical optimization surfaces enables control early aggregation from fully dissociated cells, as predicted numerical model migration, results in significant increases growth undifferentiated These chemically...