A5021342030- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Algal biology and biofuel production
- Photoreceptor and optogenetics research
- Ionic liquids properties and applications
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- ATP Synthase and ATPases Research
- Graphene research and applications
- Fullerene Chemistry and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Chemical and Physical Properties in Aqueous Solutions
- Inorganic and Organometallic Chemistry
- Fiber-reinforced polymer composites
- Mass Spectrometry Techniques and Applications
- Textile materials and evaluations
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Advanced ceramic materials synthesis
- Chemical Synthesis and Characterization
- Natural Fiber Reinforced Composites
- Radioactive element chemistry and processing
- Dyeing and Modifying Textile Fibers
- Lipid metabolism and biosynthesis
Okayama University
2015-2024
Japan Science and Technology Agency
2014-2024
Centre National de la Recherche Scientifique
2011-2024
Institut de Biologie Physico-Chimique
2010-2024
Biologie du Chloroplaste et Perception de la Lumière chez les Microalgues
2010-2024
Sorbonne Université
2010-2023
Okayama University of Science
2018-2023
National Defense Academy of Japan
2002-2021
National Academy of Education
2021
National Defence Academy
2000-2014
The carbon-concentrating mechanism (CCM) is essential to support photosynthesis under CO2-limiting conditions in aquatic photosynthetic organisms, including the green alga Chlamydomonas reinhardtii. CCM assumed be comprised of inorganic carbon transport systems that, conjunction with carbonic anhydrases, maintain high levels CO2 around ribulose-1, 5-bisphosphate carboxylase/oxygenase a specific compartment called pyrenoid. A set transcripts up-regulated during induction was identified...
Although SsrA(tmRNA)-mediated trans-translation is thought to maintain the translation capacity of bacterial cells by rescuing ribosomes stalled on messenger RNA lacking an in-frame stop codon, single disruption ssrA does not crucially hamper growth Escherichia coli. Here, we identified YhdL (renamed ArfA for alternative ribosome-rescue factor) as a factor essential viability E. coli in absence SsrA. The ssrA-arfA synthetic lethality was alleviated SsrA(DD) , SsrA variant that adds...
Flaxseed lignan secoisolariciresinol diglucoside (SDG) has been reported to prevent and alleviate lifestyle-related diseases including diabetes hypercholesterolaemic atherosclerosis. This study assesses the effect of SDG on development diet-induced obesity in mice metabolite enterodiol (END) adipogenesis 3T3-L1 adipocytes. We compared body weight, visceral fat liver content, serum parameters, mRNA levels lipid metabolism-related enzymes adiponectin fed either a low-fat diet (5 % TAG),...
Abstract The Chlamydomonas reinhardtii proton gradient regulation5 (Crpgr5) mutant shows phenotypic and functional traits similar to mutants in the Arabidopsis (Arabidopsis thaliana) ortholog, Atpgr5, providing strong evidence for conservation of PGR5-mediated cyclic electron flow (CEF). Comparing Crpgr5 with wild type, we discriminate two pathways CEF determine their maximum rates. PGR5/proton regulation-like1 (PGRL1) ferredoxin (Fd) pathway, involved recycling excess reductant increase ATP...
Significance Ca 2+ and CO 2 are fundamental biological signaling molecules in microbes, animals, plants. Although was proposed to act as a second messenger guard cells of terrestrial plants, the role signal transduction pathways aquatic photosynthetic organisms remains largely unknown. We show here that chloroplast -binding protein, CAS, changes its localization response environmental conditions regulates expression nuclear-encoded limiting-CO –induced genes, including two key bicarbonate...
In plants, the photosystem I (PSI) core complex stably associates with its light-harvesting chlorophyll a/b (LHCI) to form PSI-LHCI supercomplex. The vascular plant PSI four distinct LHCI subunits, whereas that of green alga Chlamydomonas reinhardtii binds nine subunits (LHCA1-LHCA9). stoichiometry and configuration these in supercomplex C. remain controversial. Here, we determined relative through uniform labeling total proteins using 14C. We separated polypeptides by three different sodium...
Abstract In oxygenic photosynthesis, light energy is converted into redox by two photosystems (PSI and PSII). PSI forms one of the largest multiprotein complexes in thylakoid membranes consisting a core complex, peripheral light-harvesting (LHCIs) cofactors. Although high-resolution structure PSI–LHCI complex has been determined, assembly process remains unclear due to rapid nature process. Here we show that conserved chloroplast-encoded auxiliary factors, Ycf3 Ycf4, form modules mediate...
Disulfides, with a series of alkyl spacers containing porphyrins at both ends, were prepared to evaluate the effect spacer length on interfacial structure and photoelectrochemical properties self-assembled monolayers (SAMs) gold electrode. The SAMs was investigated using UV−visible absorption spectroscopy, cyclic voltammetry, studies. These measurements showed that as increases, tend form highly ordered Photoelectrochemical studies, modified Au Pt electrodes, carried out in presence methyl...
Abstract Ycf4 is a thylakoid protein essential for the accumulation of photosystem I (PSI) in Chlamydomonas reinhardtii. Here, tandem affinity purification tagged was used to purify stable Ycf4-containing complex >1500 kD. This also contained opsin-related COP2 and PSI subunits PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, as identified by mass spectrometry (liquid chromatography–tandem spectrometry) immunoblotting. Almost all wild-type cells copurified sucrose gradient ultracentrifugation...
Photosystem I (PSI) is a multiprotein complex consisting of the PSI core and peripheral light-harvesting (LHCI) that together form PSI-LHCI supercomplex in algae higher plants. The synthesized steps during which 12-15 4-9 LHCI subunits are assembled. Here we report isolation subcomplex separated on sucrose density gradient from thylakoid membranes isolated logarithmic growth phase cells green alga Chlamydomonas reinhardtii. Pulse-chase labeling total cellular proteins revealed was de novo...
Abstract Self-assembled monolayers (SAMs) technique has been applied to porphyrin-linked fullerene systems mimic supramolecular photoinduced charge-separation (CS) events in photosynthesis. Porphyrin-linked C60 molecules with a sulfide group were designed self-assemble on gold surface using S–Au interaction. The structure of the SAMs formed electrodes was investigated by spectroscopic methods including X-ray photoelectron spectroscopy (XPS), ultraviolet (UV)-visible ellipsometry, UV-visible...
Photosynthesis is one of the most important reactions for sustaining our environment. Photosystem II (PSII) initial site photosynthetic electron transfer by water oxidation. Light in excess, however, causes simultaneous production reactive oxygen species (ROS), leading to photo-oxidative damage PSII. To maintain activity, PSII reaction center protein D1, which primary target unavoidable damage, efficiently degraded FtsH protease. In subunits, modifications several amino acids such as Trp...
H. Imahori, Yamada, S. Ozawa, Y. Sakata and K. Ushida, Chem. Commun., 1999, 1165 DOI: 10.1039/A903237A
PSII generates strong oxidants used for water oxidation.The secondary electron donor, Y Z , is Tyr161 on reaction center D1 protein and mediates transfer from the oxygen-evolving Mn 4 CaO 5 cluster to primary P680.The latest crystal structure revealed presence of a hydrogen bond network around which anticipated play important roles in proton reactions.Y forms with His190 turn Asn298 protein.Although functional have already been characterized, little known about role Asn298.Here we generated...
Chloroplast F o 1 -ATP synthase (CF CF ) converts proton motive force into chemical energy during photosynthesis. Although many studies have been done to elucidate the catalytic reaction and its regulatory mechanisms, biochemical analyses using complex limited because of various technical barriers, such as difficulty in generating mutants a low purification efficiency from spinach chloroplasts. By taking advantage powerful genetics available unicellular green alga Chlamydomonas reinhardtii ,...
Prasiola crispa , an aerial green alga, exhibits remarkable adaptability to the extreme conditions of Antarctica by forming layered colonies capable utilizing far-red light for photosynthesis. Despite a recent report on structure P. ’s unique light-harvesting chlorophyll (Chl)-binding protein complex (Pc-frLHC), which facilitates absorption and uphill excitation energy transfer photosystem II, specific genes encoding subunits Pc-frLHC have not yet been identified. Here, we draft genome...
In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. Here, we report identification of octotricopeptide repeat MTHI1, which critical for biogenesis ATP synthase oligomycin-sensitive coupling factor. Unlike most factors characterized so far in Chlamydomonas, control a single gene, MTHI1 targets two distinct transcripts: it required accumulation and translation atpH...
Photosynthesis is one of the most important reactions for sustaining our environment. Photosystem II (PSII) initial site photosynthetic electron transfer by water oxidation. Light in excess, however, causes simultaneous production reactive oxygen species (ROS), leading to photo-oxidative damage PSII. To maintain activity, PSII reaction center protein D1, which primary target unavoidable damage, efficiently degraded FtsH protease. In subunits, modifications several amino acids such as Trp...