Objective: Orangutans (Pongo abelii), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. Klebsiella pneumoniae (K. pneumonia) and other Gram-negative bacteria present in the Indigenous flora many mammals, including orangutans. This study aimed to investigate antibiotic resistance virulence profile K. pneumonia isolated from wild Sumatran Materials Methods: investigated 10 fecal samples orangutans Gunung Leuser National Park, Aceh, Indonesia....

Aim:The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid Aceh's cattle.Biodegradation by bacteria can be used as source that act degrade feed fibrous material so improve quality nutrients digestibility ingredients at cheaper price than use commercial cellulase enzymes.Materials Methods: Samples were collected cattle in Abattoirs (RPH) Banda Aceh city, Indonesia, isolation, screening done Microbiology Laboratory, Faculty Veterinary Medicine, Syiah...

This study aimed to isolate and identify Escherichia coli from broiler samples Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out considering detection antibiotic resistance genes.Cloaca swabs (n = 45) collected broilers in Isolation identification E. according standard bacteriological techniques biochemical tests, followed by confirmation polymerase chain reaction targeting uspA gene. Antibiotic sensitivity test, using several antibiotics [tetracycline...

Pasteurella multocida is a Gram-negative bacterium that causes hemorrhagic septicemia (HS) in buffaloes and cattle. The disease serious problems Indonesian livestock classified as transmissible animal disease. Previous research has determined the diversity of P. using serotyping method based on antigenic properties capsule polysaccharides. An alternative for analysis utilizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) random amplified polymorphic DNA (RAPD). This...

Abstract. Juwita S, Indrawati A, Damajanti R, Safika, Mayasari NLPI. 2021. Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia. Biodiversitas 23: 1015-1022. Antimicrobial (AMR) is an important issue affecting human animal health worldwide. This study aimed to investigate determine the S. Thirty-one isolates were tested for sensitivity 9 types antibiotics using Kirby-Bauer disk diffusion method. The...

Assessment of probiotic-supplementation on growth performance, lipid peroxidation, antioxidant capacity, and cecal microflora in broiler chickensSahar F. Deraz, Alaa E. Elkomy, Ashraf A. Khalil

A major current problem in public health is the issue of antimicrobial resistance Escherichia coli humans and poultry. In Indonesia, multidrug-resistant E. are specific concern since such may cause problems humans. The prevalence chicken strains genes, which tet(A) tet(B) were investigated present study. total 57 swabs collected from layer broiler breeder farms West Java, used experiment. Eighteen isolates identified as by disk diffusion method. classified drug-resistant intermediate then...

Necrotic enteritis causes significant losses in the global poultry industry, necessitating accurate diagnosis for effective intervention. This study aimed to develop a diagnostic tool detecting necrotic based on presence of Clostridium perfringens (C. perfringens) Alpha-toxin feces. The reagent detection kit was developed by conjugation IgG against C. toxin and Staphylococcus cells containing protein A. antibody derived from an 8-month-old thin-tailed male sheep immunized with purified 2 ml...

Aims: This study aimed to assess the risk of reproductive tract contamination in Aceh cattle by Escherichia fergusonii as revealed 16S rRNA gene sequencing preputial swab samples.Methodology and results: Preputial swabs taken from 50 breeding bulls at Indrapuri Breeding Forage Center Cattle, Banda Aceh, Indonesia, were examined for presence bacteria.Samples streaked on MacConkey agar incubated under aerobic conditions 37 °C 24 h.Smooth, yellow-or rose-colored colonies selected their...

This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.The bacterium was cattle. The total DNA the bacteria extracted using Genomic Mini Kit followed by polymerase chain reaction (PCR) amplification rRNA gene. product PCR then sequenced and aligned known sequences in GenBank database multiple alignments also analyzed bioinformatics software construct a phylogenetic tree.The results revealed that...

Orangutans are an "umbrella species" for conserving tropical forests in Sumatra and Kalimantan. There remarkable changes between the gut microbiomes of wild captive Sumatran orangutans. This study aimed to profile microbiota Nine fecal samples collected from orangutans nine were divided into three replicates. Each replicate randomly combined pieces analyzed on Illumina platform. A bioinformatics 16S rRNA according Qiime2 (Version 2021.4) microbiome profiling analysis was conducted. The...

This study aimed to isolate and identify lactic acid bacteria (LAB) in wild Sumatran orangutans provide more information about LAB diversity derived from orangutan feces.Fecal sampling three female orangutans, around 35 years old, was carried out the forest areas at research station of Suaq Belimbing Gunung Leuser National Park located South Aceh district. Orangutan fecal samples were taken morning when first defecated. The above tree, which is approximately 12-15 m ground where feces...

ABSTRAKPermasalahan resistensi Antibiotika pada hewan kesayangan menjadi kendala kesehatan di seluruh dunia. World Health Organisation (WHO) menyebutkan bahwa masa mendatang antibiotika akan tantangan yang terbesar dalam dunia kesehatan. Penelitian ini bertujuan untuk mengukur tingkat terhadap Escherichia coli diisolasi dari kucing klinik Kota Bogor. Hasil penelitian menunjukan tertinggi terjadi golongan β-laktam (ampisilin 66% dan amoksisilin 60%) diikuti oleh tetrasiklin (oksitetrasiklin...

Penelitian ini bertujuan mengetahui konsentrasi AFB1 pada jenis makanan olahan asal jagung yang dipasarkan di Banda Aceh. Pengambilan sampel dilakukan pasar dan swalayan Deteksi menggunakan metode enzyme-linked immunosorbent assay (ELISA). Berdasarkan penelitian, dapat disimpulkan bahwa dari 6 berbahan baku jagung, 5 (83,33% ) terkontaminasi AFB1. Walaupun namun semua masih aman dikonsumsi sesuai dengan persyaratan ditetapkan pemerintah yaitu maksimal 20 ppb.

Konsep Smart City hadir sebagai upaya pemanfaatan teknologi untuk mengoptimalkan pelayanan kepada masyarakat. Salah satu bentuknya yaitu berupa online berbagai urusan masyarakat oleh institusi pemerintah. Penelitian ini dilakukan mengetahui bagaimana proses adopsi inovasi via WhatsApp yang Dinasi Kependudukan dan Pencatatan Sipil (Disdukcapil) Kota Samarinda dengan menggunakan Teori Difusi Inovasi. pendekatan kualitatif deskriptif. Data diambil teknik penelitian lapangan, meliputi observasi...

Chicken is one of the important protein source in Indonesia. Moreover, largest population chicken layer and poultry Indonesia known situated at West Java province with Bogor manicipality as main producer. The aims this study were to determine antibiotic resistance pattern Staphylococcus aureus isolated from farm Bogor. also identified gene encoded resistance. Cloacal swab samples collected broiler manicipality. then cultured Mannitol Salt Agar (MSA) medium obtain S. aureus. Suspected colony...

Kampung chickens are raised using traditional production techniques by almost every household in the village.They a side-line activity and not considered main source of family earnings.The members generally working crop cultivation, as labourers, or traders.Although some families keep more than 1000 birds, they still work other activities for their livelihood.In cases, farmers have integrated native chicken operations with fresh water fish farming constructing cages above pond.This enables...

In avian ecology, the gastrointestinal microbiome is pivotal in influencing host health, contributing to metabolic processes, and shaping ecological interactions.The escalating global significance of antimicrobial resistance (AMR) genes within diverse microbiota components has garnered increasing attention due its profound consequences.Recent investigations have revealed presence antibiotic-resistant bacteria among wild bird species remote habitats, suggesting potential for these populations...

Ginseng jawa diketahui mengandung senyawa aktif yang berpotensi sebagai imunomodulator. Penelitian ini bertujuan mengidentifikasi kemampuan infusa ginseng imunomodulator berdasarkan aktivitas dan indeks fagositosis makrofag peritoneal mencit serta menentukan dosis paling efektif menggunakan 30 ekor galur DDY jantan dibagi dalam 5 kelompok, yaitu kontrol negatif (air mineral), positif (sediaan komersial), (IGJ) 33 mg/kg BB, 66 200 BB. Pemberian dilakukan satu kali sehari selama 14 hari secara...

Avian Pathogenic Escherichia coli (APEC) is a cause of extraintestinal infections, particularly respiratory pericarditis, and septicemia in poultry (colibacillosis). Research aimed at uncovering the virulence factors responsible for colibacillosis poultry, especially laying hens Indonesia, still quite rare. The purpose this study to identify E. strains resistant ciprofloxacin. targeted research are genes iss, iutA, iroN, ompT, hlyF. utilized 327 isolates from archives National Veterinary...

Antibiotic resistance is an important health problem worldwide that affects human and animal health. The presence of genes in Staphylococcus aureus Isolates may contribute to the development antibiotic resistance. study was aimed identify blaZ gene from S. were resistant penicillin G dairy farming areas South Sulawesi Province. sample obtained 20 locations farms spread across Enrekang Regency. Identification carried out by phenotypic genotypic methods. came humans (n=6), cattle (n=10),...

Orangutans are the only great apes that still survive in Southeast Asian Region. However, their population wild habitat continues to decline, with estimates indicating a 50% decreased over last 60 years. There is limited microbiological data on antibiotic resistance of Klebsiella sp. feces Bornean Orangutan (Pongo pygmaeus). This study aimed identify presence and assess its pattern various antibiotics Orangutans. The research involved isolation, identification, testing. Results showed three...

Abstract S. aureus , commonly referred to as is a bacterium that responsible for variety of infectious diseases affect human being and animal health. Target this study detect clarify phenotypically genotypically using isolates originally derived from cattle. Phenotypic identification involves assessing biochemical characteristics such Gram staining, catalase, coagulase tests. Genotypic relies on detecting the presence specific genes 23SrRNA nuc associated with through polymerase chain...

Abstract Haemorrhagic septicaemia (HS) is a significant disease in the ruminant industry tropical countries. A vaccination program for HS has been implemented Aceh, Indonesia; however, fatal cases are still frequently reported. This study aimed to determine prevalence of based on antibody titers area outbreaks and assess coverage ongoing vaccinations. One hundred seventeen blood samples from cattle buffaloes Aceh Singkil regency were collected purposively. To evaluate serum samples, an...