Ehrlichia canis and canine granulocytic sp. (CGE) infect monocytes granulocytes, respectively. E. has been cultured in vitro used to develop an immunofluorescence assay. CGE not cultured, a serologic assay is available. The sera of dogs infected with were reported react by immunofluorescence. In this study, the temporal response immunoglobulin G (IgG) was determined enzyme-linked immunosorbent (ELISA) purified antigen four experimentally canis, two CGE, one dog subsequently CGE....

The present study was designed to comparatively investigate 34 beta-hemolytic streptococci isolated from infected pigs and monkeys various islands in Indonesia. According the serological biochemical data, all isolates were Lancefield's group C could be identified as Streptococcus equi subsp. zooepidemicus. Of investigated, 28 grew on solid media large, mucoid colonies, fluid at a uniform turbidity, soft agar diffuse colonies. A decapsulation test with hyaluronidase-producing Staphylococcus...

This study aimed to isolate and identify Escherichia coli from broiler samples Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out considering detection antibiotic resistance genes.Cloaca swabs (n = 45) collected broilers in Isolation identification E. according standard bacteriological techniques biochemical tests, followed by confirmation polymerase chain reaction targeting uspA gene. Antibiotic sensitivity test, using several antibiotics [tetracycline...

Determination of the cell-surface hydrophobicity group B streptococci by hydrophobic interaction chromatography on phenyl-Sepharose revealed that human and bovine streptococcal isolates with protein surface antigens, either alone or in combination polysaccharide were mainly hydrophobic, whereas those antigens hydrophilic. Removal capsular neuraminic acid enhanced, pronase treatment reduced, hydrophobicity. The proteins, solubilized mutanolysin bacteria isolated chromatography, appeared...

Milk samples were investigated for counts and prevalence of indicator bacteria, which TPC, coliforms, coagulase positive Staphylococci (CPS), negative (CNS). Ten potential risk factors also evaluated in relation to the bacteria. The results showed that median values bacterial from overall udder-half milk 3.74, 0.70, 1.70, 2.52 log cfu/ml bulk 5.69, 2.98, 3.66 3.32 CPS, CNS, respectively. None exceeded maximum limit standards all However, only value TPC below standards. Overall CPS CNS 46.3%,...

In the present study 41 mucoid growing Streptococcus equi subsp. zooepidemicus strains (37 isolated from healthy two diseased pigs, monkeys) appeared to be phenotypically and genotypically identical S. a previously described outbreak among pig monkey population on island of Bali, Indonesia. These findings indicate that clone was still in

Deoxyribonucleic acid (DNA) vaccine has recently been developed as an alternative against virus infection.This study was the first step of DNA development to protect cyprinids including common carp (Cyprinuscarpio) and fancy koi (Cyprinus carpio) from KHV (koi herpesvirus) infection in Indonesia. One glycoproteingenes, i.e. glycoprotein (GP) ligated with Japanese medaka (Oryzias latipes) â-actin promoter generatepAct/GP a vaccine. Fourty fish body weight 10-15 g/fish were individually...

Assessment of probiotic-supplementation on growth performance, lipid peroxidation, antioxidant capacity, and cecal microflora in broiler chickensSahar F. Deraz, Alaa E. Elkomy, Ashraf A. Khalil

Rabbit erythrocytes were agglutinated by 43.4% of group B streptococci isolated from bovines but none humans. Haemagglutination was enhanced cultivation the bacteria under microaerophilic conditions. Most haemagglutinating strains had protein type antigen X, either alone, or in combination with polysaccharide antigens. Heat and proteolytic treatment destroyed haemagglutination activity. The haemagglutinin could be solubilized bacterial surface mutanolysin culture supernatant fluid ammonium...

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one appropiate ways using endophytic bacteria. The purpose this research was to isolate test antibacterial activity bacteria against two pathogenic i.e Eschericia coli Staphylococcus aureus. A total 22 isolates were obtained Based on test, 13 able inhibit growth E. 15...

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">One of major problem striped catfish <em>Pangasionodon hypophthalmus</em> culture is enteric septicemia (ESC), bacterial disease <em>Edwardsiella ictaluri</em>, caused more than 50% mortalities. This reaserch was aimed to determine pathogenicity local isolate <em>E....

Streptococcosis, as a bacterial disease with broad tropism in fish and one of the causes septicemia. Enterococcus faecalis is causative agents streptococcosis that can be isolated tilapia.This study was undertaken to complete reporting gap on pathogenicity profile clinical symptoms E. infection red tilapia (Oreochromis hybrid). The expected provide enriching information regarding recognizable signs field lead diagnosis caused by faecalis, especially Indonesian aquaculture environment.The...

Isolation and characterization of the Enterococcus faecalis strain isolated from red tilapia (Oreochromis hybrid) in Indonesia: A preliminary report

Tujuan penelitian ini adalah untuk mengidentifikasi bakteri Aeromonas hydrophila pada ikan lele dengan gejala klinis kolam bioflok di Kabupaten Muara Jambi, Provinsi Jambi. Bakteri diisolasi dari 35 ekor yang memiliki infeksi A. hydrophila. Pengambilan sampel dilakukan Purposive random sampling. Identifikasi uji biokimia berdasarkan Standar Nasional Indonesia SNI 7303.1-2015 dan konfirmasi metode PCR taget gen aerA. Hasil menujukkan bahwa sebanyak terisolasi 12 isolat biokimia. Namun...

<p>The study was aimed to determine the effectiveness of DNA vaccine doses on hematological aspect which represent immune response and its influence common carp survival rate. vaccines encoding viral glycoprotein koi herpesvirus (KHV) have been proved highly protect fish under laboratory condition. A dose 12.5 µg/100 µl had resulted in a rate 96.67 % during 30 days after challenge test with lethal KHV. Fish vaccinated using lower doses, i.e. 2.5 7.5 µg/100µl showed 100% mortality 15 19...

Brucellosis pada babi merupakan penyakit zoonosis yang disebabkan oleh bakteri Brucella suis. Penyakit ini telah terdeteksi di Indonesia, namun belum banyak diteliti. Pada penelitian dilakukan deteksi brucellosis secara serologis menggunakan metode Rose Bengal Test (RBT) dan Complement Fixation (CFT) serta molekuler teknik Polymerase Chain Reaction (PCR) AMOS untuk mengetahui spesies menginfeksi babi. Sampel berupa serum limfonodus dikoleksi dari 2 Rumah Potong Hewan Kapuk, Jakarta Ciroyom,...

Penyakit bakterial adalah salah satu penyebab kerugian besar di bidang akuakultur. Faktor virulensi bakteri penyakit umumnya diekspresikan oleh gen-gen virulen yang diregulasi dengan sistem Quorum Sensing. Tujuan dari penelitian ini untuk mendapatkan isolat memiliki potensi sebagai Anti Sensing (AQS) dapat menghambat faktor patogen pada ikan lele dumbo (Clarias gariepinus). Isolasi dilakukan sedimen, organ pencernaan dan air kolam Parung, Ciampea, Gunung Sindur, Bogor, Jawa Barat. Sebanyak...

Endophytic bacteria can produce antibacterial compounds. Their existence in the medicinal plant of Ficus variegata Blume enables production bioactive compounds similar to those contained by host plants. The purpose this study was isolate and identify endophytic from F. which is potential methods used include isolation leaves, stem, aerial root, fruit Antibacterial activity assay done against four types i.e. Staphylococcus aureus, Bacillus subtillis, Escherichia coli, Psedomonas aeruginosa....

A study have been conducted to isolate and characterize bacterial protein S. agalactiae , which is antigenic can be used test immunogenicity of vaccine in order manufacture irradiated mastitis (inflammation the udder) ruminant. The aims determine Molecular Weight (MW) irradiation, nature its caharacteristic. character stimulates antibody induction immune system, case body's defense system against disease cattle. In this study, irradiation gamma ray attenuate pathogenicity bacteria by...

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing disruption or damage to the host. Some studies suggest certain endophytic can produce chemical compounds have an effect on health, especially isolated from medicinal plants. Green betel (Piper betle L.) is a plant has been used for years and many benefits. The purposes of this study isolating screening green against four pathogenic (Staphylococcus aureus, Escherichia coli, Bacillus cereus...

ABSTRAKPermasalahan resistensi Antibiotika pada hewan kesayangan menjadi kendala kesehatan di seluruh dunia. World Health Organisation (WHO) menyebutkan bahwa masa mendatang antibiotika akan tantangan yang terbesar dalam dunia kesehatan. Penelitian ini bertujuan untuk mengukur tingkat terhadap Escherichia coli diisolasi dari kucing klinik Kota Bogor. Hasil penelitian menunjukan tertinggi terjadi golongan β-laktam (ampisilin 66% dan amoksisilin 60%) diikuti oleh tetrasiklin (oksitetrasiklin...

ABSTRACT
 
 This study aimed to conduct the characterization and molecular detection of pathogenicity antibiotic-resistance genes in Vibrio parahaemolyticus, as causative agent vibriosis Pacific white shrimp. The V. parahaemolyticus isolates were collected from shrimp’s hepatopancreas, before biochemical test polymerase chain reaction (PCR) sequencing 16S rRNA gene confirmation. hemolysis PCR applied detect presence virulence genes, namely toxR, thermostable direct haemolysin...

Chicken is one of the important protein source in Indonesia. Moreover, largest population chicken layer and poultry Indonesia known situated at West Java province with Bogor manicipality as main producer. The aims this study were to determine antibiotic resistance pattern Staphylococcus aureus isolated from farm Bogor. also identified gene encoded resistance. Cloacal swab samples collected broiler manicipality. then cultured Mannitol Salt Agar (MSA) medium obtain S. aureus. Suspected colony...