A5007303844- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- Graphene and Nanomaterials Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hydrogels: synthesis, properties, applications
- Tissue Engineering and Regenerative Medicine
ETH Zurich
2022-2024
Institute for Biomedical Engineering
2022-2024
Abstract Digital light processing (DLP) of structurally complex poly(ethylene glycol) (PEG) hydrogels with high mechanical toughness represents a long‐standing challenge in the field 3D printing. Here, we report printing approach for high‐resolution manufacturing and mechanically strong PEG via heat‐assisted DLP. Instead using aqueous solutions photo‐crosslinkable monomers, macromonomer melts were first printed absence water, resulting bulk networks. Then, post‐printing swelling networks was...
Generating 3D bone cell networks in vitro that mimic the dynamic process during early formation remains challenging. Here, we report a synthetic biodegradable microporous hydrogel for efficient of from human primary cells, analysis cell-secreted extracellular matrix (ECM) and microfluidic integration. Using polymerization-induced phase separation, demonstrate situ microporosity (5-20 µm) within metalloproteinase-degradable polyethylene glycol hydrogels presence living cells. Pore is...
3D osteocyte cultures reveal that fast stress-relaxing hydrogels enhance early morphogenesis, while slow-relaxing favor osteogenic differentiation after 14 days, highlighting their mechanosensitivity to matrix mechanics.
Digital light processing (DLP) of poly(ethylene glycol) (PEG) hydrogels with high mechanical toughness represents a long-standing challenge in the 3D printing field. Here, we report approach for high-resolution manufacturing structurally complex and mechanically strong PEG via heat-assisted DLP. Instead using aqueous solutions photocrosslinkable monomers, macromonomer melts were first printed absence water, resulting bulk networks. Then, post-printing swelling networks was achieved producing...
Abstract Generating 3D bone cell networks in vitro that accurately mimic the dynamic process of osteoblast embedding during early formation poses a significant challenge. Herein, we report synthetic biodegradable macroporous hydrogel for efficient from human primary cells, analysis cell-secreted extracellular matrix (ECM) and microfluidic integration. Using polymerization-induced phase separation, metalloproteinase-sensitive polyethylene glycol hydrogels are formed with interconnected...
The functionality of many biological tissues relies on their highly sophisticated architecture. Recent advances have enabled in vitro generation human organoid models through 3D stem cell culture animal-derived protein hydrogels. However, these oversimplified materials often lack vivo-like microarchitecture and mechanical stimuli to support tissue formation. As such, there is an imperative need develop architected hydrogels that can be integrated with microfluidic technologies provide...