• The high-throughput phenotypic analysis of Arabidopsis thaliana collections requires methodological progress and automation. Methods to impose stable reproducible soil water deficits are presented were used analyse plant responses stress. Several potential complications difficulties identified, including the spatial temporal variability micrometeorological conditions within a growth chamber, difference in depletion rates between accessions differences developmental stage same time after...

Abstract Growth and carbon (C) fluxes are severely altered in plants exposed to soil water deficit. Correspondingly, it has been suggested that under deficit suffer from C shortage. In this study, we test hypothesis Arabidopsis (Arabidopsis thaliana) by providing an overview of the responses growth, balance, metabolites, enzymes central metabolism, a set sugar-responsive genes sustained The results show drought, rosette relative expansion rate is decreased more than photosynthesis, leading...

Abstract Leaf expansion is the central process by which plants colonize space, allowing energy capture and carbon acquisition. Water emerge as main limiting factors of leaf expansion, but literature remains controversial about their respective contributions. Here, we tested hypothesis that importance hydraulics metabolics organized according to both dark/light fluctuations ontogeny. For this purpose, established developmental pattern individual during days nights in model plant Arabidopsis...

RNA editing plays an important role in organelle gene expression various organisms, including flowering plants, changing the nucleotide information at precise sites. Here, we present evidence that maize (Zea mays) nuclear Pentatricopeptide repeat 2263 (PPR2263) encoding a DYW domain–containing PPR protein is required for mitochondrial NADH dehydrogenase5 (nad5) and cytochrome b (cob) transcripts nad5-1550 cob-908 sites, respectively. Its putative ortholog, MITOCHONDRIAL EDITING FACTOR29,...

Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness yield decrease. The maximum quantum efficiency photosystem II (F v/F m) is a parameter chlorophyll fluorescence (ChlF) classically used track changes in performance. Despite recent technical methodological advances ChlF imaging, the spatio-temporal heterogeneity F m still awaits for standardized accurate quantification.

ABSTRACT Genetic variability in the plasticity of leaf area expansion response to water deficit has been reported Arabidopsis thaliana . Here, objective was identify underlying dynamic and cellular processes involved this variability. Twenty‐five accessions were subjected identical soil treatments. In all accessions, production low compared with that individual expansion. A subset selected for further dissection into its variables: rate duration epidermal cell number area. had opposite...

Summary The formation of Casparian strips (CS) and the deposition suberin at endodermis plant roots are thought to limit apoplastic transport water ions. We investigated specific role each these barriers in control hydro‐mineral by consequences on shoot growth. A collection Arabidopsis thaliana mutants defective and/or CS development was characterized under standard conditions using a hydroponic system Phenopsis platform. Mutants altered had enhanced root hydraulic conductivity, indicating...

Abstract Both leaf production and expansion are tightly linked to cell division, but the functional relationships between all these variables not clearly established. To get insight into relationships, a quantitative genetic analysis was performed in 118 recombinant inbred lines derived from cross Landsberg erecta Antwerp accessions combined with structural equation modeling approach. Main effects epistatic interactions at trait locus (QTL) level were detected for rosette area, number, 6...

Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manual, often destructive operations, causing large errors. imaging emerged as a viable alternative allowing non-invasive automated acquisition. Several procedures based on image analysis developed monitor leaf growth major target. However, in most proposals, time-consuming parameterization...

Variation in leaf development caused by water deficit was analysed 120 recombinant inbred lines derived from two Arabidopsis thaliana accessions, Ler and An-1. Main effect quantitative trait loci (QTLs) QTLs epistatic interactions were mapped for the responses of rosette area, number 6 area to deficit. An interaction between affected response whole individual but only with effects well-watered condition. A second controlled specific These validated generating phenotyping new appropriate...

Abstract Enormous progress has been achieved understanding the molecular mechanisms regulating endoreduplication. By contrast, how this process is coordinated with cell cycle or expansion and contributes to overall growth in multicellular systems remains unclear. A holistic approach was used here give insight into functional links between endoreduplication, division, expansion, whole Arabidopsis (Arabidopsis thaliana) leaf. Correlative analyses, quantitative genetics, structural equation...

Mutualistic bacteria can alter plant phenotypes and confer new abilities to plants. Some growth-promoting rhizobacteria (PGPR) are known improve both growth tolerance multiple stresses, including drought, but reports on their effects survival under severe water deficits scarce. We investigated the effect of Phyllobacterium brassicacearum STM196 strain, a PGPR isolated from rhizosphere oilseed rape, survival, physiological responses Arabidopsis thaliana combining destructive non-destructive...

The regulation of the GORK (Guard Cell Outward Rectifying) Shaker channel mediating a massive K+ efflux in Arabidopsis guard cells by phosphatase AtPP2CA was investigated. Unlike gork mutant, atpp2ca mutants displayed phenotype reduced transpiration. We found that interacts physically with and inhibits activity Xenopus oocytes. Several amino acid substitutions active site, including dominant interfering G145D mutation, disrupted GORK-AtPP2CA interaction, meaning native conformation site is...

How genetic factors control plant performance under stressful environmental conditions is a central question in ecology and for crop breeding. A multivariate framework was developed to examine the architecture of performance-related traits response interacting stresses. Ecophysiological life history were quantified Arabidopsis thaliana Ler×Cvi mapping population exposed constant soil water deficit high air temperature. The plasticity variance–covariance matrix (G-matrix) examined using...

Inorganic phosphate (Pi) and Zinc (Zn) are essential nutrients for normal plant growth. Interaction between these elements has been observed in many crop plants. Despite its agronomic importance, the biological significance genetic basis of this interaction remain largely unknown. Here we examined Pi/Zn two lettuce ( Lactuca sativa ) varieties, namely, “Paris Island Cos” “Kordaat.” The effects variation Pi Zn supply were assessed on biomass photosynthesis each variety. Paris Cos displayed...

Plants suffer from a broad range of abiotic and biotic stresses that do not occur in isolation but often simultaneously. Productivity natural agricultural systems is frequently constrained by water limitation, the frequency duration drought periods will likely increase due to global climate change. In addition, phytoviruses represent highly prevalent threat wild cultivated plant species. Several hints support modification epidemiological parameters viruses response environmental changes...

It is clearly established that there not a unique response to soil water deficit but are as many responses characteristics: Drought intensity, drought duration, and position during plant cycle. For same deficit, can also differ on genotype within species. In spite of this variability, at least for leaf production expansion processes, robust tendencies be extracted from the literature when similar watering regimes compared. Here, we present curves multi-scale dynamics analyses tomato plants...

Light and soil water content affect leaf surface area expansion through modifications in epidermal cell numbers area, while effects on thickness mesophyll volumes are far less documented. Here, three-dimensional imaging was applied a study of Arabidopsis thaliana growth to determine the cellular organization tissues under moderate deficit two cumulative light conditions. In contrast highly conserved response both low high regimes. Unlike palisade tissues, no reductions number were observed...

Water stresses reduce plant growth but there is no consensus on whether carbon metabolism has any role in this reduction. Sugar starvation resulting from stomatal closure often proposed as a cause of impairment under long-term or severe water deficits. However, decreases faster than photosynthesis response to drought, leading increased carbohydrate stores short-term moderate Here, we addressed the question availability deficits using two different systems. Firstly, monitored day/night...

Acclimation to water deficit (WD) enables plants maintain growth under unfavorable environmental conditions, although the mechanisms are not completely understood. In this study, natural variation of long-term acclimation moderate and severe soil WD was investigated in 18 Arabidopsis (Arabidopsis thaliana) accessions using PHENOPSIS, an automated phenotyping platform. Soil content adjusted at early stage plant development maintained a constant level until reproductive age achieved. The were...

Following the recent development of high-throughput phenotyping platforms for plant research, number individual plants grown together in a same experiment has raised, sometimes at expense pot size. However, root restriction excessively small pots affects growth and carbon partitioning, may interact with other stresses targeted these experiments. In work reported here, we investigated interactive effects size soil water deficit on multiple growth-related traits from cellular to whole-plant...

Plant virus pathogenicity is expected to vary with changes in the abiotic environment that affect plant physiology. Conversely, viruses can alter host response additional stimuli from antagonism mutualism depending on virus, and environment. Ecological theory, specifically CSR framework of strategies developed by Grime collaborators, states plants cannot simultaneously optimize resistance both water deficit pathogens. Here, we investigated vegetative reproductive performance 44 natural...

Abstract In leaves of C3 and C4 plants, stomata open during the day to favor CO2 entry for photosynthesis close at night prevent inefficient transpiration water vapor. The circadian clock paces rhythmic stomatal movements throughout diel (24–h) cycle. Leaf transitory starch is also thought regulate movements, yet underlying mechanisms across time (key moments) space (relevant leaf tissues) remain elusive. Here, we developed PhenoLeaks, a pipeline analyze dynamics transpiration, used it...

Aim: Nitrogen is a major element conditioning grapevine growth, yield and aromatic profiles of berries wines. Different tools can be used in order to detect differences N status the plant, including direct measurements soil, plant nitrogen (eg. petiole; must), or indirect observations nutritional such as leaf transmittance reflectance SPAD; NDVI). However, relationships between these indicators overall functioning over vintages remain poorly known. The present study aimed at quantifying key...