A5080759248- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- NF-κB Signaling Pathways
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Adipokines, Inflammation, and Metabolic Diseases
- CRISPR and Genetic Engineering
- Phagocytosis and Immune Regulation
- T-cell and Retrovirus Studies
- Mycobacterium research and diagnosis
- Animal Genetics and Reproduction
- Chronic Myeloid Leukemia Treatments
- Reproductive System and Pregnancy
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Platelet Disorders and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Growth Hormone and Insulin-like Growth Factors
- HIV Research and Treatment
- interferon and immune responses
- Erythrocyte Function and Pathophysiology
Dokkyo Medical University
2017-2025
Hokkaido University
2002-2017
Creative Research Enterprises (United States)
2016
Indiana University School of Medicine
2013
Institute of Environmental Biology and Biotechnology
2013
Immune Regulation (United Kingdom)
2007-2008
RIKEN Center for Integrative Medical Sciences
2004-2006
RIKEN
2004-2005
Chiba University
2003-2004
Toho University
2004
Previous observations have shown that binding of growth hormone to its receptor leads activation transcription factors via a mechanism involving phosphorylation on tyrosine residues. In order establish whether the prolactin-activated factor Stat 5 (mammary gland factor) is also activated by hormone, nuclear extracts were prepared from COS-7 cells transiently expressing transfected and cDNA. Gel electrophoresis mobility shift analyses revealed hormone-dependent presence specific DNA-binding...
Mammary epithelial cells, under the regulation of lactogenic hormones, produce high amounts milk proteins during lactation period. The caseins are most abundant proteins. We have studied beta-casein gene expression and found that hormones induce transcription promoter. hormonal is mediated in part by a mammary gland-specific factor (MGF). MGF specific DNA-binding protein which recognizes sequence 5‘-ACTTCT-TGGAATT-3‘. This conserved with slight variations alpha- promoters cow rodents at...
NKT cells expressing the invariant Vα14 antigen receptor constitute a novel lymphocyte subpopulation with immunoregulatory functions. Stimulation via their anti‐CD3 or ligand, α‐galactosylceramide (α‐GalCer), triggers activation of cells, resulting in rapid cytokine production such as IFN‐γ and IL‐4. Soon after activation, disappeared judged by staining CD1d tetramer loaded α‐GalCer (α‐GalCer/CD1d tetramer), which has been believed to be due apoptotic cell death. Here we show that...
Extracellular stimuli trigger adipocyte differentiation by inducing the complex cascades of transcription. Transcription factors CCAAT enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ) play crucial roles in this process. Although ectopic expression these NIH-3T3 cells, a multipotential mesenchymal stem cell line, results adipogenic conversion, little is known as to hormonal that regulate adipogenesis cells. In report we demonstrate PRL, lactogenic...
Signal transducer and activator of transcription 5 (Stat5) mediates signaling many cytokines growth factors. Here we show that Stat5 functions as an initial mediator adipogenesis. The preadipocyte cell line 3T3-L1 undergoes adipocyte differentiation upon appropriate hormonal induction. We found Stat5A Stat5B were strongly activated at early stage differentiation. To investigate physiological roles in adipogenesis, have constructed lines which either exogenous wild type (wt) or dominant...
Abstract Vα14 NKT cells exhibit various immune regulatory properties in vivo, but their precise mechanisms remain to be solved. In this study, we demonstrate the of generation dendritic (DCs) by stimulation vivo. After repeated injection α-galactosylceramide (α-GalCer) into mice, splenic DCs acquired IL-10-dependent fashion, such as nonmatured phenotypes and increased IL-10 reduced IL-12 production. The unique cytokine profile these appears regulated ERK1/2 IκBNS. These also showed an...
Thrombopoietin (TPO) is a hematopoietic growth factor that plays fundamental roles both megakaryopoiesis and thrombopoiesis through binding to its receptor, c-mpl. Although TPO has been shown activate various types of intracellular signaling molecules, such as the Janus family protein tyrosine kinases, signal transducers activators transcription (STATs), ras, precise mechanisms underlying TPO-induced proliferation differentiation remain unknown. In an effort clarify differentiation, c-mpl...
Abstract It is well-documented that certain chemokines or their receptors play important roles in the graft rejection. However, of and maintenance transplantation tolerance remain unclear. In this study, we demonstrate blocking interaction between chemokine receptor, CXCR6, highly expressed on Vα14+ NKT cells its ligand, CXCL16, resulted failure to maintain thus induction acceleration a mouse transplant model, expression CXCL16 was up-regulated tolerated allografts, anti-CXCL16 mAb inhibited...
Mucosal-associated invariant T (MAIT) cells belong to a family of innate-like that bridge innate and adaptive immunities. Although MAIT have been implicated in tumor immunity, it currently remains unclear whether they function as tumor-promoting or inhibitory cells. Therefore, we herein used induced pluripotent stem cell (iPSC) technology investigate this issue. Murine were reprogrammed into iPSCs redifferentiated towards MAIT-like (m-reMAIT cells). m-reMAIT activated by an agonist the...
The function of mucosal-associated invariant T (MAIT) cells, a burgeoning member innate-like cells abundant in humans and implicated many diseases, remains obscure. To explore this, mice with rearranged cell receptor (TCR) α or β locus, specific for MAIT were generated via induced pluripotent stem derived from designated Vα19 Vβ8 mice, respectively. Both groups expressed large numbers cells. these activated by cytokines an agonist to produce IFN-γ IL-17. While showed resistance cancer...