A5030463138- Bacterial Infections and Vaccines
- Legionella and Acanthamoeba research
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Reproductive tract infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antifungal resistance and susceptibility
- Urinary Tract Infections Management
- Enterobacteriaceae and Cronobacter Research
- Vector-borne infectious diseases
- Infections and bacterial resistance
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Syphilis Diagnosis and Treatment
- Bartonella species infections research
- Diphtheria, Corynebacterium, and Tetanus
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
Maasstad Ziekenhuis
2011-2019
University of California, Santa Barbara
2003-2016
Elisabeth-TweeSteden Ziekenhuis
1999-2014
St. Elisabeth Hospital
1993-2007
Flemish Community
2005
University of Antwerp
2005
Province of Antwerp
2005
National Institute for Public Health and the Environment
1993-1997
Laboratory confirmation of pertussis by culture, PCR, or detection antibody increase in paired sera is hampered low sensitivity the later stages disease. Therefore, we investigated whether, and at which level, concentrations immunoglobulin G (IgG) antibodies against toxin (PT), IgG-PT, a single serum sample are indicative active recent pertussis. measured enzyme-linked immunosorbent assay units per milliliter, was analyzed 7,756 collected population-based study The Netherlands, 3,491...
ABSTRACT We describe the clinical and laboratory features of a 55-year-old human immunodeficiency virus-negative female patient who presented with bilateral intraocular inflammatory disease (neuroretinitis type) behavioral changes caused by Bartonella grahamii infection. Diagnosis was based on PCR analysis DNA extracted from fluids. product revealed 100% identity 16S rRNA gene sequence B. . The successfully treated doxycycline (200 mg/day) rifampin (600 for 4 weeks. This is first report that...
Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up patients an outbreak setting, a molecular screening method was developed for detection the most prevalent genes; blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC. A real-time multiplex PCR assay evaluated using collection 86 Gram negative isolates, including 62 producers....
A total of 188 Bordetella strains were characterized by the electrophoretic mobilities 15 metabolic enzymes and distribution variation in positions copy numbers three insertion sequences (IS). The presence or absence IS elements within certain lineages was congruent with estimates overall genetic relationships as revealed multilocus enzyme electrophoresis. pertussis ovine B. parapertussis each formed separate clusters, while human most closely related to IS1001-containing bronchiseptica...
ABSTRACT PCR is increasingly being used as a diagnostic test for the detection of Bordetella pertussis and parapertussis DNA, it has improved sensitivity specificity in comparison to conventional techniques. The assay described here uses two insertion sequences IS 481 1001 B. , respectively, with by molecular beacons. real-time detects both holmesii . By performing assays discrimination between can be obtained. was 1 10 CFU/ml assays. clinical not affected duplexing an internal control PCR....
On 31 May 2011, after notification of Klebsiella pneumoniae (KP)(OXA-48;CTX-M-15) in two patients, nosocomial transmission was suspected a Dutch hospital. Hospital-wide infection control measures and an outbreak investigation were initiated. A total 72,147 patients categorised into groups based on risk OXA-48 colonisation or infection, 7,527 screened for Enterobacteriaceae(OXA-48) by polymerase chain reaction (PCR). Stored KP isolates (n=408) retrospectively tested CTX-M-1 group...
The aim of this study was to validate the performance polymerase chain reaction (PCR) assay for Bordetella pertussis and parapertussis in comparison with both culture serology. number samples positive PCR 2A-fold higher than culture. In serologically confirmed cases, sensitivity depended on duration disease age patient, being less sensitive older later disease. patients < 10 days symptoms 70%, 50%, 10% groups <1 year, 1–4 years, ⩾5 respectively. Evidence suggested that effect may be due...
A polymerase chain reaction (PCR) assay which allows the simultaneous detection and discrimination of two causative agents pertussis, Bordetella pertussis parapertussis, was developed. Primer pairs were based on insertion sequence elements IS481 IS1001. is specific for B. present in about 80 copies per cell, while IS1001 parapertussis found 20 cell. An internal control included PCR to monitor performance identify possible inhibitory components clinical samples. Discrimination amplified DNA...
In Dutch laboratories molecular detection of B. pertussis and parapertussis is commonly based on insertion sequences IS481 IS1001, respectively. Both IS elements are more widely spread among Bordetella species. holmesii, bronchiseptica can harbour IS481. Also, IS1001 found bronchiseptica. IS481, PCR thus lacks specificity when used for specific spp.We designed a IS1002, another element that present species, exploited it as template in combination with IS1001. combining the PCRs including an...
The objective of our study was the development a semi-quantitative real-time PCR to detect uropathogens. Two multiplex reactions were designed Escherichia coli, Klebsiella spp., Enterobacter Citrobacter Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S based performed in parallel Gram-positive Gram-negative bacteria. Firstly identify non-targeted agents infection same urine specimen, secondly quantify background flora. method evaluated comparison with standard...
The emergence of carbapenem-resistant Pseudomonas aeruginosa represents a worldwide problem. To understand the carbapenem-resistance mechanisms and their spreading among P. strains, whole genome sequences were determined two extensively drug-resistant strains that are endemic in Dutch hospitals. Strain Carb01 63 is O-antigen serotype O12 sequence type ST111, whilst S04 90 O11 strain ST446. Both carry gene for metallo-β-lactamase VIM-2 flanked by aacA29 genes encoding aminoglycoside...
ABSTRACT Rapid and efficient epidemiologic typing systems would be useful to monitor transmission of methicillin-resistant Staphylococcus aureus (MRSA) at both local interregional levels. To evaluate the intralaboratory performance interlaboratory reproducibility three recently developed repeat-element PCR (rep-PCR) methods for MRSA, 50 MRSA strains characterized by pulsed-field gel electrophoresis (PFGE) ( Sma I) analysis epidemiological data were blindly typed inter-IS 256 , 16S-23S...
ABSTRACT Repetitive sequence-based (Rep)-PCR genotyping as described here is based on the presence of homologues Mycoplasma pneumoniae repeat-like elements in Staphylococcus . In this study we comparatively evaluated usefulness rep-PCR typing with two sets well-defined collections aureus strains. Rep-PCR analysis first collection S. strains ( n = 59) and one intermedius strain showed 14 different patterns, each pattern harboring 6 to 15 DNA fragments. The discriminatory power compared well...
Bordetella holmesii is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms humans. B. contains insertion sequences IS481 and IS1001, two frequently used targets the PCR diagnosis of pertussis parapertussis infections. To investigate prevalence Finnish Dutch patients with whether has caused any false-positive results diagnostic PCRs, holmesii-specific real-time PCRs were developed. The methods conventional (LightCycler, Roche) targeting recA gene. IS1001...
We describe a novel insertion sequence (IS) element, IS1002, which was found to be closely related IS481, is only in Bordetella pertussis; we that these two IS eleemnts have level of identity 61.5% and also almost identical terminal inverted repeats. IS1002 present both B. pertussis parapertussis strains isolated from humans. In contrast, absent sheep. A DNA fingerprint analysis performed with anothe IS1001, bronchiseptica, revealed isolates obtained sheep are distinct human isolates. Thus,...
Several reports describe the use of Qiagen columns as part a Legionella PCR ([1][1], [2][2]). However, workers in two laboratories The Netherlands have found that are contaminated with species. Nucleic acid sample preparation kits commercially available from
By analysis of repetitive DNA in Bordetella parapertussis, an insertion sequence element, designated IS1001, was identified. Sequence revealed that IS1001 comprised 1,306 bp and contained inverted repeats at its termini. Furthermore, several open reading frames may code for transposition functions were The largest frame coded a protein comprising 406 amino acid residues showed homology to TnpA, which is encoded by element (IS1096) found Mycobacterium smegmatis. Examination flanking sequences...
ABSTRACT Although the PCR for detection of Bordetella pertussis is routinely performed in diagnostic laboratories, no quality assessment program has so far been described. We report on results obtained with two external proficiency panels sent to European laboratories. The first panel contained a series dilutions three previously characterized B. clinical isolates and negative controls. No false-positive were reported by six laboratories providing seven data sets. limits strains varied...
Objectives. A large OXA-48 outbreak in the Netherlands involved spread of OXA-48producing Enterobacteriaceae among at least 118 patients, suggesting horizontal transfer this resistance gene through one or more plasmids. Elucidating transmission dynamics plasmids is hampered by low resolution classic typing methods. This study aimed to investigate molecular epidemiology carrying carbapenemase using a next-generation sequencing approach.Methods. total 68 OXA-48-producing isolated from hospital...
The effect of temporal and geographic factors on the population structure Bordetella pertussis was studied using IS1002-based RFLP analysis. Among 106 strains analysed, 36 different types were observed. For Dutch strains, there evidence for a shift in time since majority found families related successive periods. Most pronounced differences observed between 1950-1954 later This difference may have been caused by introduction whole-cell vaccine 1953, resulting expansion which are less...
The object of our study was to determine the proportion atypical respiratory pathogens among patients hospitalized with a community-acquired infection. From September 1997 May 1999, 159 (57% male, median age 55, range 1-88 y) admitted 3 regional hospitals for community acquired infection, were enrolled in study. Microbiological diagnosis Mycoplasma pneumoniae, Chlamydia and Legionella pneumophila performed PCR on throat swab, sputum and/or broncho alveolar lavage (BAL). In addition, species...
Legionella pneumonia can be difficult to diagnose. Existing laboratory tests all have shortcomings, especially in the ability diagnose Legionnaires' disease (LD) at an early stage of a specimen that is readily obtainable. The aim this study was assess performance PCR as rapid diagnostic method and compare results different assays serum samples from patients with LD. Samples included 151 68 proven LD 60 36 respiratory tract infections other than Legionella. were based on 5S rRNA gene, 16S...
ABSTRACT An outbreak with a multiresistant Klebsiella pneumoniae (MRKP) strain among seven patients admitted to the adult intensive care unit (ICU) of regional teaching hospital in The Netherlands was investigated. Epidemiologic investigations revealed short delay between an operation and acquisition MRKP strain. A case-control study comprising 7 cases 14 controls conducted identify risk factors associated at each two rooms strongly strain: odds ratio 36 (95% confidence interval, 2.7 481.2;...