A5003148292- Zebrafish Biomedical Research Applications
- Invertebrate Immune Response Mechanisms
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- Ocular Oncology and Treatments
- Cardiac Valve Diseases and Treatments
- Cardiomyopathy and Myosin Studies
- Extracellular vesicles in disease
- Congenital heart defects research
- Click Chemistry and Applications
- Proteoglycans and glycosaminoglycans research
- Angiogenesis and VEGF in Cancer
- Cerebrospinal fluid and hydrocephalus
- Wnt/β-catenin signaling in development and cancer
- Retinal and Macular Surgery
- Ubiquitin and proteasome pathways
University of California, San Diego
2015-2023
University of California, San Francisco
2008-2014
Broad Center
2011
Knowing how mutations disrupt the interplay between atrioventricular valve (AVV) morphogenesis and function is crucial for understanding congenital defects arise. Here, we use high-speed fluorescence microscopy to investigate AVV in zebrafish at cellular resolution. We find that leaflets form directly through a process of invagination, rather than first forming endocardial cushions. There are three phases embryonic development. First, canal (AVC) closed by mechanical action myocardium, rolls...
Epithelial stem cells are maintained within niches that promote self-renewal by providing signals specify the cell fate. In Drosophila ovary, epithelial follicle (FSCs) reside in at anterior tip of tissue and support continuous growth ovarian epithelium. Here, we demonstrate a neighboring dynamic population stromal cells, called escort FSC niche cells. We show produce both Wingless Hedgehog ligands for lineage, signaling is specific whereas active FSCs daughter addition, multiple...
Development of the dorsal aorta is a key step in establishment adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium all vertebrate animals studied. Work zebrafish has demonstrated that arterial venous endothelial precursors distinct subsets lateral plate mesoderm. Here, we profile transcriptome earliest detectable (ECs) during embryogenesis to demonstrate tissue-specific EC programs initiate much earlier than previously...
The Drosophila melanogaster genetic tool box includes many stocks for generating genetically mosaic tissue in which a clone of cells, related by lineage, contain common alteration. These tools have made it possible to study the postembryonic function essential genes and better understand how individual cells interact within intact tissues. We screened through 201 enhancer-trap flippase lines identify that produce useful patterns adult ovary. found approximately 70% produced clones were...
Abstract Development of the dorsal aorta is a key step in establishment adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium all vertebrate animals studied. Work zebrafish has demonstrated that arterial venous endothelial precursors distinct subsets lateral plate mesoderm. Earlier studies chick showed paraxial mesoderm generates another subset incorporate into to replace HSPCs as they exit enter circulation. Here we show...
Abstract Dynamic turnover of cell‐surface glycans is involved in a myriad biological events, making this process an attractive target for vivo molecular imaging. Metabolic glycan labeling coupled with bioorthogonal chemistry has paved the way visualizing living organisms. However, two‐step sequence required, which suffers from tissue‐penetration difficulties imaging probes. Here, by exploring substrate promiscuity endogenous glycosyltransferases, we developed single‐step fluorescent strategy...
ABSTRACT Dynamic turnover of cell-surface glycans is involved in a myriad biological events, making this process an attractive target for vivo molecular imaging. The metabolic glycan labeling coupled with ‘bioorthogonal chemistry’ has paved the way visulizing living organisms. However, two-step sequence required, which prone to tissue penetration difficulties imaging probes. Here, by exploring substrate promiscuity endogenous glycosyltransferases, we developed single-step fluorescent...