A5035419700- Muscle Physiology and Disorders
- Ion channel regulation and function
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Cardiovascular Disease and Adiposity
- Neurobiology and Insect Physiology Research
- Inflammasome and immune disorders
- Heat shock proteins research
- Connexins and lens biology
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle activation and electromyography studies
- Dietary Effects on Health
- Biochemical effects in animals
- Cardiovascular Effects of Exercise
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Takotsubo Cardiomyopathy and Associated Phenomena
- Laser Applications in Dentistry and Medicine
- Long-Term Effects of COVID-19
- Hormonal and reproductive studies
- Autophagy in Disease and Therapy
- Liver Disease Diagnosis and Treatment
University of Chile
2009-2024
University of Valparaíso
2019-2024
Universidad de Ciencias Medicas
2018
Fundación Universitaria San Pablo CEU
2012
Purpose The anabolic hormone testosterone induces muscle hypertrophy, but the intracellular mechanisms involved are poorly known. We addressed question whether signal transduction pathways other than androgen receptor (AR) necessary to elicit hypertrophy in skeletal myotubes. Methods Cultured rat myotubes were preincubated with inhibitors for ERK1/2 (PD98059), PI3K/Akt (LY294002 and Akt inhibitor VIII) or mTOR/S6K1 (rapamycin), then stimulated 100 nM testosterone. expression of α-actin...
Tetanic electrical stimulation induces two separate calcium signals in rat skeletal myotubes, a fast one, dependent on Cav 1.1 or dihydropyridine receptors (DHPRs) and ryanodine related to contraction, slow signal, DHPR inositol trisphosphate (IP3Rs) transcriptional events. We searched for adult muscle fibers using isolated flexor digitorum brevis from 5–7-wk-old mice, loaded with fluo-3. When stimulated trains of 0.3-ms pulses at various frequencies, cells responded signal associated...
Summary An important pending question in neuromuscular biology is how skeletal muscle cells decipher the stimulation pattern coming from motoneurons to define their phenotype as slow or fast twitch fibers. We have previously shown that voltage-gated L-type calcium channel (Cav1.1) acts a voltage sensor for activation of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P3]-dependent Ca2+ signals regulates gene expression. ATP released by after electrical through pannexin-1 channels plays key role...
Low-grade chronic inflammation plays a pivotal role in the pathogenesis of insulin resistance (IR), and skeletal muscle has central this condition. NLRP3 inflammasome activation pathways promote low-grade several tissues. However, direct link between IR not been reported. Here, we evaluated components their GLUT4 translocation impairment during IR. Male C57BL/6J mice were fed with normal control diet (NCD) or high-fat (HFD) for 8 weeks. The protein levels NLRP3, ASC, caspase-1, gasdermin-D...
A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat leads accumulation of a large number intrahepatic lipid droplets (LD), which are susceptible oxidation. Obesity also affects muscle, increasing LD producing insulin signaling impairment. Physalis peruviana L. (PP) (Solanaceae) is rich peruvioses has high antioxidant activity. We assessed ability PP enhance insulin-dependent glucose uptake muscle capacity prevent both...
Heat shock proteins (HSPs) are a conserved family of cytoprotective polypeptides, synthesized by cells in response to stress. Hsp70 and heme oxygenase 1 (Hmox-1) induced variety cellular stressors skeletal muscle, playing role long-term adaptations muscle fibers regeneration. Though HSPs expression after exercise has been intensely investigated, the molecular mechanisms concerning Hmox-1 induction poorly understood. The aim this work was investigate involvement calcium upon depolarization...
Obesity is a pandemic caused by many factors, including chronic excess in hypercaloric and high-palatable food intake. In addition, the global prevalence of obesity has increased all age categories, such as children, adolescents, adults. However, at neurobiological level, how neural circuits regulate hedonic consumption intake reward circuit modified under diet are still being unraveled. We aimed to determine molecular functional changes dopaminergic glutamatergic modulation nucleus...
Duchenne muscular dystrophy (DMD) is a neuromuscular disease originated by reduced or no expression of dystrophin, cytoskeletal protein that provides structural integrity to muscle fibres. A promising pharmacological treatment for DMD aims increase the level dystrophin homolog called utrophin. Neuregulin-1 (NRG-1), growth factor potentiates myogenesis, induces utrophin in skeletal cells. Microarray analysis total gene allowed us determine neuregulin-1β (NRG-1β) one 150 differentially...
Abstract Objective To establish recommendations through the consensus of a Latin American experts panel on use flash glucose monitoring system (fCGM) in people living with type 2 diabetes mellitus (T2DM) regarding benefits and challenges using fCGM. Methods An executive committee was created, comprised by fifteen physicians, including endocrinologists internal medicine expertise management adult patients T2DM. The were from various countries: Colombia, Chile, Peru, Mexico, Argentina, Brazil....
The gut-brain axis is a bidirectional communication pathway that modulates cognitive function. A dysfunctional has been associated with impairments during aging. Therefore, we propose evaluating whether modulation of the gut microbiota through fecal transplantation (FMT) from young-trained donors (YT) to middle-aged or aged mice could enhance brain function and cognition in old age. Twelve-month-old male received an initial FMT YT (YT-Tr) age-matched (Auto-Tr) following antibiotic treatment....
One of the most important functions skeletal muscle is to respond nerve stimuli by contracting. This function ensures body movement but also participates in other physiological roles, like regulation glucose homeostasis. Muscle activity closely regulated adapt different demands and shows a plasticity that relies on both transcriptional stimuli. These two processes, dependent depolarization plasma membrane, have so far been regarded as separated independent processes due lack evidence common...
Non-alcoholic fatty liver disease (NAFLD) is the most common in Western countries, with its prevalence increasing annually [...]
An important question in neuromuscular biology is how skeletal muscle cells decipher the stimulation pattern coming from motoneurons to define their phenotype-activating transcriptional changes a process named excitation-transcription coupling. We have shown adult fibers that 20 Hz electrical (ES) activates signaling cascade starts with Cav1.1 activation, ATP release trough pannexin-1 channel, activation of purinergic receptors, and IP3-dependent Ca2+ signals inducing related plasticity fast...
In adult muscle fibers, Cav1.1 acts as voltage sensor for both excitation‐contraction coupling and the activation of a signaling cascade that regulates gene expression. We have shown ATP is released through pannexin‐1 channels after electrical stimulation at 20 Hz, having key role in induction transcriptional changes related to fast‐to‐slow fiber phenotype transition. Myotubes lacking Cav1.1α1 subunit displayed almost no release stimulation. transcription was also altered fibers knock‐down...
An important pending question in neuromuscular biology is how skeletal muscle cells decipher the stimulation pattern coming from motoneurons to define their phenotype as slow or fast twitch fibers; we realized that fiber signals through extracellular ATP regulate cellular processes, including regulating changes. In adult fibers, 20 Hz electrical activates a signaling cascade starts with Cav1.1 activation, release trough pannexin channels, purinergic receptors activation and IP3‐dependent...
In skeletal myofibers, transcription of a specific group genes is regulated by different stimulation frequencies, process known as muscle plasticity. We described IP 3 ‐dependent calcium signals after slow frequency which were dependent on Cav1.1 and ATP release through pannexin channels. Phosphatidyl inositol kinase (PI3K) one the downstream proteins involved in activation this signaling pathway. Here we present central role for PI3K studied expression sarcomeric (slow fast troponin I)...