A5054475810- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Metal-Catalyzed Oxygenation Mechanisms
- Plant nutrient uptake and metabolism
- Carbohydrate Chemistry and Synthesis
- Digestive system and related health
- Advanced Thermodynamics and Statistical Mechanics
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Advanced Cellulose Research Studies
- Enzyme Structure and Function
- Electrochemical sensors and biosensors
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Listeria monocytogenes in Food Safety
- Alzheimer's disease research and treatments
- Aquaculture disease management and microbiota
- Microbial metabolism and enzyme function
Instituto de Agroquímica y Tecnología de Alimentos
2011-2024
Universitat de València
2006-2024
Consejo Superior de Investigaciones Científicas
2010-2024
Unidades Centrales Científico-Técnicas
2013
University of Gothenburg
2011
Zero to Three
2010
Scripps Research Institute
2007
University of Lisbon
2006
Microbial communities from cow rumen are known for their ability to degrade diverse plant polymers at high rates. In this work, we identified 15 hydrolases through an activity-centred metagenome analysis of a fibre-adherent microbial community dairy rumen. Among them, 7 glycosyl (GHs) and 1 feruloyl esterase were successfully cloned, expressed, purified characterised. The most striking result was protein GH family 43 (GHF43), hereinafter designated as R_09-02, which had characteristics very...
Treatment of purified Rubisco with agents that specifically oxidize cysteine-thiol groups causes catalytic inactivation and increased proteolytic sensitivity the enzyme. It has been suggested these redox properties may sustain a mechanism regulating activity turnover during senescence or stress. Current research efforts are addressing structural basis modulation identification critical cysteines. Redox shifts result in conformational changes as revealed by alteration its fragmentation...
The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm adapted a dynamic situation such as to stress, calculate theoretical decay from experimental data. Yeast genes have grouped into 25 clusters according level rate kinetics, average calculated each cluster. In most genes, changes one or both experimentally determined parameters occur during response. 24% are...
Efficient enzymatic synthesis of tailor-made prebiotic fructo-oligosaccharides (FOS) used in functional food formulation is a relevant biotechnological objective. We have engineered the Saccharomyces cerevisiae invertase (Suc2) to improve its transferase activity and identify determinants for product specificity. Amino acid replacement (W19Y, N21S, N24S) within conserved motif (β-fructosidase) specifically increased 6-kestose up 10-fold. Mutants with lower substrate (sucrose) affinity...
ABSTRACT The role of cysteines 449 (Cys449) and 459 (Cys459) from the large subunit (LS) ribulose 1·5‐bisphosphate carboxylase/oxygenase (Rubisco) in reduction–oxidation (redox) regulation enzyme was assessed by site‐directed mutagenesis these residues chloroplast transformation Chlamydomonas reinhardtii . In vitro studies indicated that mutations C449S, C459S or C449S/C459S do not affect activity proteolytic susceptibility reduced state. However, when oxidized, mutant enzymes differed wild...
In this work we report a detailed analysis of the topology and phylogenetics family 2 glycoside hydrolases (GH2). We distinguish five topologies or domain architectures based on presence distribution protein domains defined in Pfam Interpro databases. All them share central TIM barrel (catalytic module) with two β-sandwich (non-catalytic) at N-terminal end, but differ occurrence nature additional non-catalytic modules C-terminal region. Phylogenetic was sequence Glyco_hydro_2_C catalytic...
Glucose oxidase is one of the most conspicuous commercial enzymes due to its many different applications in diverse industries such as food, chemical, energy and textile. Among these applications, remarkable manufacture glucose biosensors particular sensor strips used measure levels serum. The generation ameliorated versions therefore a significant biotechnological objective. We have strategy that combined random rational approaches isolate uncharacterized mutations Aspergillus niger with...
Metagenomics has opened up a vast pool of genes for putative, yet uncharacterized, enzymes. It widens our knowledge on the enzyme diversity world and discloses new families which clear classification is still needed, as exemplified by glycoside hydrolase family-3 (GH3) proteins. Herein, we describe GH3 (GlyA1) from resident microbial communities in strained ruminal fluid. The β-glucosidase/β-xylosidase that also shows β-galactosidase, β-fucosidase, α-arabinofuranosidase,...
Glycoside hydrolases, specifically β-galactosidases, can be used to synthesize galacto-oligosaccharides (GOS) due the transglycosylating (secondary) activity of these enzymes. Site-directed mutagenesis a thermoresistant β-galactosidase from Thermotoga maritima has been carried out study structural basis transgalactosylation and obtain enzymatic variants with better performance for GOS biosynthesis. Rational design mutations was based on homologous sequence analysis modeling. Analysis mutant...
Ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) plays a central metabolic role in photosynthetic eukaryotes, and its catabolism is crucial process for the nutrient economy of higher plants. The rubisco holoenzyme assembled from eight chloroplast-encoded large subunits nuclear-encoded small subunits. We have identified cluster conserved tyrosines at interface between (comprising Y67, Y68, Y72 βA−βB loop subunit Y226 subunit) that may contribute to stability. To investigate these...
The α-glucosidase encoded by the aglA gene of Aspergillus niger is a secreted enzyme belonging to family 31 glycoside hydrolases. This has retaining mechanism action and displays transglycosylating activity that makes it amenable be used for synthesis isomaltooligosaccharides (IMOs). We have expressed in Saccharomyces cerevisiae under control galactose-inducible promoter. Recombinant yeast cells expressing produced extracellular about half which appeared cell bound whereas other was released...
Lactose intolerance is a common digestive disorder that affects large proportion of the adult human population. The severity symptoms highly variable, depending on susceptibility to sugar and amount digested. For reason, enzymes can be used for production lactose-free milk derivatives have acquired singular biotechnological importance. One such case Thermotoga maritima β-galactosidase (TmLac). Here, we report cryo-EM structure TmLac at 2.0 Å resolution. protein features newly solved domain...
Abstract Background Xylanases are one of the most extensively used enzymes for biomass digestion. However, in many instances, their use is limited by poor performance under conditions pH and temperature required industry. Therefore, search xylanases able to function efficiently at alkaline high an important objective different processes that lignocellulosic substrates, such as production paper pulp biofuels. Results A comprehensive silico analysis family GH11 sequences from CAZY database...