A5085122970- Fungal and yeast genetics research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- BRCA gene mutations in cancer
- Advanced biosensing and bioanalysis techniques
- Carcinogens and Genotoxicity Assessment
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Telomeres, Telomerase, and Senescence
- Spaceflight effects on biology
- Aging and Gerontology Research
- FOXO transcription factor regulation
Biomedical Research Institute of Lleida
2011-2022
Universitat de Lleida
2008-2022
Hospital de Sant Pau
2021
Universitat Autònoma de Barcelona
2021
University of Cologne
2014-2017
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2015-2017
Faculty (United Kingdom)
2015
We have analyzed the heat stress response in yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for whole transcriptome after a shift from 25°C to 37°C. Using an established mathematical algorithm, theoretical decay also been calculated experimental data. verified predictions selected genes their at different times during using regulatable tetO promoter. This study indicates that shock is not only due changes rates, but stabilities. stability affected 62% of it...
The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm adapted a dynamic situation such as to stress, calculate theoretical decay from experimental data. Yeast genes have grouped into 25 clusters according level rate kinetics, average calculated each cluster. In most genes, changes one or both experimentally determined parameters occur during response. 24% are...
DNA damage causally contributes to aging and age-related diseases. Mutations in nucleotide excision repair (NER) genes cause highly complex congenital syndromes characterized by growth retardation, cancer susceptibility, accelerated humans. Orthologous mutations Caenorhabditis elegans lead delay, genome instability, functional decline, thus allowing investigation of the consequences persistent during development a simple metazoan model. Here, we conducted proteome, lipidome, phosphoproteome...
Saccharomyces cerevisiae can import iron through a high-affinity system consisting of the Ftr1/Fet3-mediated reductive pathway and siderophore-mediated non-reductive one. Expression components is controlled by Aft1 transcriptional factor. In this study we show that, upon oxidative stress, transitorily internalized into nucleus, followed transcription activation its regulon. these conditions, mRNA levels genes become increased, while those FTR1 FET3 remain low because destabilization mRNAs....
Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism iron starvation conditions by promoting decay of the targeted molecules, order to avoid excess consumption. This study shows absence immediate upregulation expression several regulon genes (involved high affinity uptake and intracellular redistribution) upon oxidative stress hydroperoxide more intense than wild type where present. The provokes a temporary increase levels (itself member regulon). In such...
Abstract The tumor suppressor FANCD1/BRCA2 is crucial for DNA homologous recombination repair (HRR). BRCA2 biallelic pathogenic variants result in a severe form of Fanconi anemia (FA) syndrome, whereas monoallelic cause mainly hereditary breast and ovarian cancer predisposition. For decades, the co-occurrence trans with clearly variant led to assume that other allele was benign. However, here we show patient (c.1813dup c.7796 A > G) diagnosed at age 33 FA after hypertoxic reaction...