- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Lipid metabolism and biosynthesis
- CRISPR and Genetic Engineering
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- RNA regulation and disease
- RNA and protein synthesis mechanisms
Max Planck Institute of Molecular Plant Physiology
2019-2024
Key proteins of the photosynthetic complexes are encoded in chloroplast genome and cotranslationally inserted into thylakoid membrane. However, molecular details this process largely unknown. Here, we demonstrate by ribosome profiling that conserved signal recognition particle subunit (cpSRP54) is required for efficient cotranslational targeting several central proteins, such as PSII PsbA (D1) subunit, Arabidopsis (Arabidopsis thaliana). High-resolution analysis membrane-associated soluble...
Abstract Photosystem I (PSI) forms a large macromolecular complex of ∼580 kDa that resides in the thylakoid membrane and mediates photosynthetic electron transfer. PSI is composed 18 protein subunits nearly 200 co-factors. The assembly membranes requires high spatial temporal coordination, critically dependent on sophisticated machinery. Here, we report characterize CO-EXPRESSED WITH ASSEMBLY1 (CEPA1), factor Arabidopsis (Arabidopsis thaliana). CEPA1 gene was identified bioinformatically as...
The initial step of oxygenic photosynthesis is the thermodynamically challenging extraction electrons from water and release molecular oxygen. This light-driven process, which basis for most life on Earth, catalyzed by photosystem II (PSII) within thylakoid membrane photosynthetic organisms. biogenesis PSII requires a controlled step-wise assembly process early steps are considered to be highly conserved between plants their cyanobacterial progenitors. involves auxiliary proteins, likewise...
RNA editing converts cytidines to uridines in plant organellar transcripts. Editing typically restores codons for conserved amino acids. During evolution, specific C-to-U sites can be lost from some lineages by genomic C-to-T mutations. By contrast, the emergence of novel is less well documented. are recognized pentatricopeptide repeat (PPR) proteins with high specificity. recognition PPR partially predictable, but prediction often inadequate PPRs involved editing. Here we have characterized...
ABSTRACT The initial step of oxygenic photosynthesis is the thermodynamically challenging extraction electrons from water and release molecular oxygen. This light-driven process, which basis life on Earth, catalyzed by photosystem II (PSII) within thylakoid membrane photosynthetic organisms. biogenesis PSII requires a controlled step-wise assembly process early steps are considered to be highly conserved between plants their cyanobacterial progenitors. involves auxiliary proteins, likewise...