Propolis is one of the most used natural products known for centuries its beneficial effects. Due to significant differences in chemical composition samples originating from different geographic and climatic zones it crucial characterize reliably each type propolis. This article comprises latest findings concerning poplar propolis, i.e. gives a cross section composition, botanical origin biological activity propolis order encourage further investigations that would indicate

Abstract Introduction Propolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee‐product exhibits numerous biological activities, including anti‐bacterial, anti‐viral, anti‐inflammatory, anti‐oxidant, immunostimulating and anti‐tumour effects. As chemical composition activity propolis depend on its botanical geographical origin, searching for new bioactive substances in types unexplored regions great...

High‐performance thin‐layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting classification of 52 propolis samples collected from Serbia one sample Croatia. Modern equipment in combination software processing warping was applied data acquisition. The three mostly chemometric techniques classification, principal component analysis, cluster partial least square‐discriminant simple fast HPTLC method fingerprint propolis, performed...

The free amino acids profile of 192 samples seven different floral types Serbian honey (acacia, linden, sunflower, rape, basil, giant goldenrod, and buckwheat) from six regions was analysed in order to distinguish honeys by their botanical origin.The most abundant were proline, alanine, phenylalanine, threonine arginine. Based on the established profiles, some important differences have been identified among studied relying basic descriptive statistics data, confirmed multivariate...

Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed combat infectious diseases. Here we report new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 μM). Through detailed structure-activity study, have identified 7-Cl...

This study investigates the impact of Nosema infection in beehives on physico-chemical and biochemical properties spectral characteristics honey as indicators quality. Comprehensive analyses were performed samples from hives with varying levels infection, examining water content, free acidity, optical rotation, electrical conductivity, sugar composition, catalase activity, pollen content. Honey highly infected showed higher content (up to 17.3%), lower reduced decreased glucose levels,...

Lipophilicity is a vital physicochemical parameter of molecule, which affects several biological processes such as absorption, tissue distribution, and pharmacokinetic properties. In this study, evaluation lipophilicities series novel fluoroquinolone-Safirinium dye hybrids using chromatographic computational methods presented. Fluoroquinolone-Safirinium have been synthesized new dual-acting hydrophilic antibacterial agents. Reversed phase thin-layer chromatography micellar electrokinetic...

Contemporary metabolomic fingerprinting is based on multiple spectrometric and chromatographic signals, used either alone or combined with structural chemical information of metabolic markers at the qualitative semiquantitative level. However, signal shifting, convolution, matrix effects may compromise patterns. Recent increase in use data, described by presence (1) absence (0) particular metabolites, demonstrates great potential field profiling fingerprint analysis. The aim this study a...

The lipophilicity of a molecule is well-recognized as crucial physicochemical factor that conditions the biological activity drug candidate. This study was aimed to evaluate isoxazolo[3,4-b]pyridine-3(1H)-ones and their N1-substituted derivatives, which demonstrated pronounced antifungal activities. Several methods, including reversed-phase thin layer chromatography (RP-TLC), reversed phase high-performance liquid (RP-HPLC), micellar electrokinetic (MEKC), were employed. Furthermore,...

Domesticated international (standard) apple cultivars, together with resistant cultivars are the core of Serbian production. Furthermore, autochthonous characterized by a good adaptability to local environmental conditions and represent valuable source genetic variability, as well an important gene pool for further breeding programs. Additionally, they show higher phenolic content stronger antioxidant activity, in comparison commercial cultivars. Therefore, more likely be used functional...

A quantitative structure-retention relationship study has been performed to correlate the retention of 33 newly synthesized arylpiperazines with their molecular characteristics, using thin-layer chromatography. Principal component analysis followed by multiple linear regression (MLR), principal (PCR) and partial least squares (PLS) was identify most important factors, quantify influences, select descriptors that best describe behavior compounds investigated. The statistical performance...

Both quantitative structure-retention (QSRR) and structure-activity relationship (QSAR) studies have been performed to correlate the molecular characteristics of seven pairs cis-trans isomeric bis-steroidal tetraoxanes with their reversed-phase thin-layer chromatography (RPTLC) retention as well antiproliferative activity. 2D 3D descriptors whole molecule representations together parameters biological activity data were subjected multivariate statistical analysis (principal component...

In the current article, an overview of recent applications and opportunities image evaluation high-performance thin-layer chromatograms (HPTLC) in food analysis natural product research is presented. The article shortly covers aspects specialized software packages for analysis, post-correction, signal acquisition, preprocessing. Contemporary image-based HPTLC fingerprinting, quantification target compounds, advanced chemometric modeling were reviewed. Special attention was dedicated to...

Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It used for screening viable candidates. Chromatographic behavior thiepino[3,2-c:6,7-c']dipyridine 16 benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions two microemulsion chromatographic systems. Seventeen nine in silico lipophilicity measures were estimated. They compared by classical...