A5061010723- Helminth infection and control
- Agricultural safety and regulations
- Coccidia and coccidiosis research
- Parasites and Host Interactions
- Advanced Proteomics Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Glutathione Transferases and Polymorphisms
- Metabolomics and Mass Spectrometry Studies
- Parasite Biology and Host Interactions
- Mitochondrial Function and Pathology
- Dupuytren's Contracture and Treatments
- Trace Elements in Health
- Nail Diseases and Treatments
- Bone fractures and treatments
- Orthopedic Surgery and Rehabilitation
- Parasitic Diseases Research and Treatment
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Network Time Synchronization Technologies
- Biotin and Related Studies
- Heat shock proteins research
- Rabies epidemiology and control
- Plant Pathogens and Fungal Diseases
- Parasitic infections in humans and animals
- Ultrasound in Clinical Applications
University College Dublin
2024
University of Massachusetts Chan Medical School
2023
Park Terrace Care Center
2023
Croda (United Kingdom)
2022
Monaghan Biosciences (Ireland)
2017
Unilever (United Kingdom)
2017
Liverpool School of Tropical Medicine
2008-2014
Dublin City University
1992-2014
QIMR Berghofer Medical Research Institute
1994-2000
Stony Brook University
1995-1996
Background Insecticide-based methods represent the most effective means of blocking transmission vector borne diseases. However, insecticide resistance poses a serious threat and there is need for tools, such as diagnostic tests detection, that will improve sustainability control interventions. The development tools metabolism-based in mosquito vectors lags behind those target site mutations. Methodology/Principal Findings We have developed validated simple colorimetric assay detection...
The development of resistance to insecticides has become a classic exemplar evolution occurring within human time scales. In this study we demonstrate how DDT in the major African malaria vector Anopheles gambiae is result both target-site mechanisms that have introgressed between incipient species (the M- and S-molecular forms) allelic variants DDT-detoxifying enzyme. Sequencing detoxification enzyme, Gste2, from resistant susceptible strains An. gambiae, revealed non-synonymous...
The invasive stages of the parasitic trematode Fasciola hepatica release proteinases into medium in which they are maintained. In this study, we investigated interaction F. excretory/secretory (E/S) products and 2 cysteine (CL1 CL2) purified from these with extracellular matrix basement membrane macromolecules. E/S contained collagenolytic activity on fibrillar types I III collagen as well type IV collagen. CL1 CL2 were capable degrading acid-soluble but not insoluble contrast, neither nor...
A 29.5‐kDa cysteine proteinase was purified from medium in which mature Fasciola hepatica parasites were maintained. The N‐terminal sequence (14 residues) of the protein is similar to known cathepsin L proteinases, including a 27‐kDa proteinase, also secreted by this parasite, had been isolated previously our laboratory [Smith, A. M., Dowd, J., Mc Gonigle, S., Keegan, P. Brennan, G., Trudgett, & Dalton, J. (1993) Mol. Biochem. Parasitol. 62 , 1–8]. sequences and proteinases differ only...
Our laboratory recently developed a diagnostic test (ELISA) for human fascioliasis based on the detection of serum IgG4 antibodies reactive with Fasciola hepatica cathepsin L1 (CL1). In present study, we have used recombinant CL1, generated by functional expression cDNA in Saccharomyces cerevisiae, this immunodiagnostic and compared its performance native CL1. Sera obtained from 64 individuals living Cutusuma village northern Altiplano Bolivia, region high prevalence fascioliasis, were...
ABSTRACT Cysteine proteinases expressed by schistosomes appear to play key roles in the digestion of host hemoglobin, principal source amino acid nutrients utilized these parasites. We have shown previously that predominant cysteine proteinase activity soluble extracts and excretory/secretory (ES) products adults Schistosoma mansoni S. japonicum is cathepsin L-like its substrate specificity. However, biochemical analysis L ES has been complicated presence at least two distinct forms...
The glucose-sensitive insulin-secretion (GSIS) phenotype is relatively unstable in long-term culture of beta cells. purpose this study was to investigate relative changes the proteome between glucose-responsive (low passage) and glucose non-responsive (high murine MIN-6 pancreatic 2D-DIGE subsequent DeCyder analysis detected 3351 protein spots pH range 4–7. Comparing MIN-6(H) MIN-6(L) using a threshold 1.2-fold, number proteins with decrease expression level 152 (4.5%), similar 3140 (93.7%)...
A cDNA encoding the complete precursor of a Fasciola hepatica cathepsin L protease was isolated and sequenced. Functionally active enzyme expressed secreted by Saccharomyces cerevisiae transformed with plasmid carrying gene. Experiments temperature‐sensitive yeast mutants showed that is trafficked through secretory pathway. Yeast truncated gene, which lacked pre‐peptide‐encoding most pro‐peptide‐encoding sequences, did not express funtionally enzyme. The yeast‐expressed exhibited...
A cathepsin L proteinase secreted by the parasitic helminth Fasciola hepatica can cleave fibrinogen and produce a fibrin clot with specific activity of 4.7 National Institutes Health thrombin‐equivalent U/mg. This is first report fibrinogen‐clotting aside that thrombin snake venom proteinases, which are all serine proteinases. Clot formation not inhibited inhibitor hirudin or anti‐polymerant H‐Gly‐Pro‐Arg‐Pro‐OH. The enzyme exerts its on in unique manner. Although cleavage may involve...
The zoonotic hookworm, Ancylostoma caninum, probably induces human eosinophilic enteritis by inducing allergic responses to its secretions. This species is already known secrete metalloproteinases, but in other parasites, cysteine proteinases are involved pathogenesis. We studied somatic extracts of A. caninum adults and infective larvae adult excretory/secretory (ES) antigens for proteinase activity using fluorogenic peptide substrates gelatin substrate polyacrylamide gel electrophoresis....
SUMMARY A dipeptidylpeptidase (DPP) was isolated from Fasciola hepatica by gel-filtration and ion-exchange chromatography. The exoproteinase is secreted newly excysted juveniles, immature mature flukes. liver fluke DPP a serine proteinase of molecular weight > 200 kDa differs previously characterized mammalian DPPs in its substrate preference susceptibility to inactivation inhibitors. parasite may function the latter stages proteolytic digestion host macromolecules. In this manner, enzyme...
Fasciola hepatica secretes a cathepsin L proteinase that is suggested to play an in vivo role immunoprotection since the enzyme can cleave host immunoglobulin. In present report, rabbit anti-cathepsin IgG was shown bind and inhibit its ability molecules. Cathepsin prevent antibody-mediated attachment of eosinophils newly excysted juveniles vitro assays; however, if are mixed with prior addition assay, attach juveniles. Thus it possible prepare antibodies disrupt biological activity F. L.
We report genome sequences of two new isolates the budding yeast