A5055282757- Research on Leishmaniasis Studies
- Blood Coagulation and Thrombosis Mechanisms
- Trypanosoma species research and implications
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Barrier Structure and Function Studies
- Organic Chemistry Cycloaddition Reactions
- Coccidia and coccidiosis research
- Fluorine in Organic Chemistry
- Axial and Atropisomeric Chirality Synthesis
- Agricultural safety and regulations
- Erythrocyte Function and Pathophysiology
- Peptidase Inhibition and Analysis
- Diamond and Carbon-based Materials Research
- Ubiquitin and proteasome pathways
- Parasites and Host Interactions
- Enzyme Production and Characterization
- Pluripotent Stem Cells Research
- Synthesis and Properties of Aromatic Compounds
- Helminth infection and control
- Inorganic Fluorides and Related Compounds
- Organoselenium and organotellurium chemistry
- Asymmetric Synthesis and Catalysis
- Metallurgical and Alloy Processes
University of Maryland, Baltimore
2018-2025
University of Baltimore
2023
University of Glasgow
1987-1999
Wellcome Centre for Molecular Parasitology
1992-1999
General Electric (United States)
1983-1987
SUMMARY A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigotelike forms in Schneider's Drosophila medium supplemented with 2% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture parasites obtained from lesions infected animals rather than adaptation promastigotes vitro . Ultrastructural (possession megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) infectivity (...
Despite the abundance of capillary thin-strand pericytes and their proximity to neurons glia, little is known contributions these cells control brain hemodynamics. We demonstrate that pharmacological activation pericyte KATP channels profoundly hyperpolarizes cells, dilates upstream penetrating arterioles arteriole-proximate capillaries, increases blood flow. Focal stimulation with a channel agonist sufficient evoke this response, mediated via KIR2.1 channel-dependent retrograde propagation...
Summary We have isolated a gene encoding previously unreported class of trypanosomatid cysteine proteinase (CP) from the protozoan parasite Leishmania mexicana. The single‐copy ( Imcpa ) has several unusual features that distinguish it CP genes cloned related species Trypanosoma brucei and cruzi. These include shorter C ‐terminal extension only 10 amino acids three‐amino‐acid insertion, GlyValMet, close to predicted N ‐terminus mature protein. Northern blot analysis showed is expressed in...
Cas9 targets genomic loci with high specificity. For knockin double-strand break repair, however, often leads to unintended on-target knockout rather than intended edits. This imprecision is a barrier for direct
A method comprising enzyme separation by SDS-PAGE and subsequent use of peptidyl aminomethylcoumarins as substrates has been used to study proteinases the protozoan parasite Trypanosoma brucei. The application this allowed investigation substrate specificities individual in cell lysates without need for purification. results show that T. brucei contains a group cysteine proteinases, probably four number, with inhibitor similar those cathepsin L. second larger enzymes significantly different...
Four distinct bands of cysteine proteinase activity were detected when stationary-phase populations Leishmania mexicana subjected to gelatin-SDS-PAGE. The highest mobility band contained at least three isoforms separable by mono Q anion exchange chromatography. These high activities from all the major amastigote enzymes. Stationary-phase promastigote also two acid-activable precursor forms promastigote-specific band. It is suggested that these occur in infective metacyclic stage parasite and...
The title isomeric hosts (1) and (2), adopting different molecular conformations, form distinct types of closed cage accommodating the common guest 1,4-dioxane; it is remarkable that symmetrical multimolecular structure (1), not consolidated by hydrogen bonding, can exist even in absence guest.
Summary: The interrelationship of the multiple cysteine proteases (CPs) found characteristically at high activity in Leishmania mexicana amastigotes has been investigated. mature forms five enzymes groups B and C, which have subtly different substrate preferences, are same size. Enzymically deglycosylated group A CP proteins also molecular mass. Proteases all three specifically recognized by antisera raised against or C CPs. In addition, CPs A, highly similar N-terminal amino acid sequences....
The expression of cysteine proteinases by metacyclic promastigotes Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable proteinase activity, and amastigotes, with a distinct banding pattern composed multiple enzymes. A correlation between relative activity the metacyclic-specific prevalence found both during growth cycle in vitro as...
ABSTRACT Enteric pathogens with low infectious doses rely on the ability to orchestrate expression of virulence and metabolism-associated genes in response environmental cues for successful infection. Accordingly, human pathogen enterohemorrhagic Escherichia coli (EHEC) employs a complex multifaceted regulatory network link type III secretion system (T3SS) components nutrient availability. While phosphorylation histidine aspartate residues two-component regulators is recognized as an...
SUMMARY Capillary pericytes and their processes cover ∼90% of the total length brains capillary bed. Despite abundance, little is known pericyte function, contributions to control brain hemodynamics remain unclear. Here, we report that deep possess a mechanistic ‘energy switch’ that, when activated by decrease in glucose, elicits robust K ATP channel activation increase blood flow protect energy substrate availability. We demonstrate pharmacological channels profoundly hyperpolarizes leads...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation B. D. Hunt, N. Lewis, E. L. Hall, C. Robertson; Summary Abstract: Control of pinhole formation in epitaxial CoSi2 films. J. Vac. Sci. Technol. B 1 May 1987; 5 (3): 749–750. https://doi.org/10.1116/1.583782 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks...